Linking the physiologic and phylogenetic successions in free‐living bacterial communities along an estuarine salinity gradient

Abstract

In this paper we assess whether the bacterial compositional succession that occurs along an estuarine salinity gradient is accompanied by changes in both community and single-cell metabolic activity in the free-living bacterioplankton. In addition, we explore whether up-and downstream estuarine communities, which are dominated by different phylogenetic groups, show distinct patterns in single-cell activity and characteristics. We have characterized the physiological succession of the bacterioplankton along the salinity gradient in the Choptank River Estuary (Maryland), using a combination of indices of single-cell activity, which include respiratory activity, membrane polarization and integrity, and DNA and RNA contents, combined with flow cytometry. We have also measured bacterioplankton community production, respiration, and growth efficiency along the estuary. Our data suggest that the sharp phylogenetic succession that occurs within the fresh to saltwater transition region is accompanied by profound metabolic changes both at the single-cell and community levels and that the phylogenetic succession occurs together with measurable physiological stress. The different indices of single-cell characteristics that we have used converge to suggest that within the fresh to saltwater transition zone, there is generalized decline in growth and possibly a loss of activity and even significant cell mortality. At the community level, these changes in singlecell physiology at the site of the phylogenetic succession appear to translate into a generalized decline in bacterial growth efficiency with a decrease in bacterial growth and production but an actual increase in bacterial carbon consumption. Our data also suggest that different phylogenetic groups may have intrinsically different levels of single-cell activity or at least respond differently to the activity assays that we currently use.There are now a relatively large number of published studies that have described the bacterial composition in a range of aquatic ecosystems by use of a variety of molecular techniques. Overall, these studies have shown, for example, that bacterial communities in fresh-and saltwater systems are often dominated by very different taxonomic groups, even at the broadest phylogenetic levels (Glöckner et al. 1999;Giovanonni and Rappé 2000). The bacterial phylogenetic succession that occurs along environmental gradients, such as salinity gradients, has received considerably less attention than the description of the phylogenetic composition in individual systems. There are at least two fundamental aspects of the phylogenetic succession in bacterioplankton assemblages along environmental gradients that remain to be explored. First, it is still unclear whether the succession proceeds mostly as a physical replacement of major phylogenetic groups or whether the succession proceeds through activation/inactivation of the various groups that are already present in the assemblage. Second, it is still unclear whether 1 Present address: Dépt des ...

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