Linoleic Acid—A Feasible Preventive Approach for Visceral Leishmaniasis

Saini, Sharanjot; Kumar, Ambak

doi:10.3389/fnut.2021.649025

Cited by 6 publications

(9 citation statements)

References 36 publications

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“…Nevertheless, we found enrichment of linoleic acid (LA) metabolism in LmexCen −/− immunized mice, compared to mice infected with Lmex WT. It has been recently shown that LA inhibits L. donovani survival in macrophages and promotes production of superoxide by neutrophils and macrophages 29 . Furthermore, LA is a precursor of ω-6 polyunsaturated fatty acids (PUFAs), and deficiency of PUFAs impairs cell-to-cell interaction, resulting in improper antigen presentation and inadequate lymphocyte activation 29 .…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, LA is a precursor of ω-6 polyunsaturated fatty acids (PUFAs), and deficiency of PUFAs impairs cell-to-cell interaction, resulting in improper antigen presentation and inadequate lymphocyte activation 29 . Thus, the intake of appropriate amounts of LA through diet can make a difference between resistance and susceptibility to L. donovani in patients 29 . Although these results have not been validated in other species of Leishmania , they may affect mechanisms of vaccine immunity.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the t scores for the matched metabolites, this pathway was activated in the LmexCen -/--group. This pathway has previously been shown to promote a protective immune response in visceral leishmaniasis, and could provide a mechanism of protection in the lymph nodes 29 . We also found several other pathways that were uniquely or consistently enriched in the draining lymph nodes of LmexCen -/--immunized and LmexWTinfected mice, compared to the naïve group (Supplementary Figure 3).…”

Section: Interestingly Mass Spectrometry Analysis Of Lymph Node Tissu...mentioning

confidence: 94%

“…It has been recently shown that LA inhibits L. donovani survival in macrophages and promotes production of superoxide by neutrophils and macrophages 29 . Furthermore, LA is a precursor of ω-6 polyunsaturated fatty acids (PUFAs), and deficiency of PUFAs impairs cell-to-cell interaction, resulting in improper antigen presentation and inadequate lymphocyte activation 29 . Thus, the intake of appropriate amounts of LA through diet can make a difference between resistance and susceptibility to L. donovani in patients 29 .…”

Section: Although a Lot Of Attention Has Been Given To The Role Of Ch...mentioning

confidence: 99%

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Leishmania mexicana Centrin Knock out Parasites Promote M1-polarizing Metabolic Changes

Volpedo

Oljuskin²,

Azodi³

et al. 2022

Preprint

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Leishmaniasis is a tropical disease present in more than 90 countries. Presently, there is no approved vaccine for human use. We have previously developed live attenuated L. mexicana Cen-/- (LmexCen-/-) as a vaccine candidate that showed excellent efficacy that was characterized by reduced activation of Th2 responses and enhanced Th1 responses, contrary to wild type L. mexicana (LmexWT) infection. Towards understanding the interplay between immune mechanisms of protection and metabolic reprogramming, we applied untargeted mass spectrometric analysis to LmexCen-/- and compared them with LmexWT infection. Data showed that enriched pentose phosphate pathway (PPP) in ears immunized with LmexCen-/- parasites, compared to naive and LmexWT-infected ears. This pathway is known to promote an M1 phenotype in macrophages, suggesting a switch to a pro-inflammatory phenotype following LmexCen-/- inoculation. Accordingly, inhibition of the PPP in macrophages cultured with LmexCen-/- parasites led to diminished production of nitric oxide, IL-12, and IL-1beta, hallmarks of classical activation. Overall, our study revealed novel immune regulatory mechanisms that may be critical for the induction of protective immunity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Interestingly Mass Spectrometry Analysis Of Lymph Node Tissu...mentioning

confidence: 94%

Section: Although a Lot Of Attention Has Been Given To The Role Of Ch...mentioning

confidence: 99%

See 2 more Smart Citations

Leishmania mexicana Centrin Knock out Parasites Promote M1-polarizing Metabolic Changes

Volpedo

Oljuskin²,

Azodi³

et al. 2022

Preprint

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“…Linoleic acid and linolenic acid are cell components and precursors of prostaglandins that participate in phospholipid synthesis. Also, they are closely related to cholesterol transport, which is crucial in regulating human physiological functions and safeguarding physical health ( Fitzsimons et al, 2019 ; Samson et al, 2020 ; Saini and Rai, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)

et al. 2021

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Foxtail millet (Setaria italica) as the main traditional crop in China, is rich in many kinds of high quality fatty acids (FAs). In this study, Ultra-high performance liquid chromatography-time-of-flight-tandem mass spectrometer (UHPLC-Q-TOF-MS/MS) was used to determine the lipids of JG35 and JG39. A total of 2,633 lipid molecules and 31 lipid subclasses were identified, mainly including thirteen kinds of glycerophospholipids (GP), eleven kinds of glycerolipids (GL), four kinds of sphingolipids (SP), two kinds of fatty acyls (FA) and one kind of sterol (ST). Among them JG35 had higher contents of diacylglycerols (DG) and ceramides (Cer), while triacylglycerols, phosphatidyl ethanolamine, phosphatidic acid, sterol, fatty acyls and pardiolipin (TG, PE, PA, ST, FA and CL) were higher in JG39. Meantime, the correlation analysis of lipidomics and transcriptomics was used to map the main differential lipid metabolism pathways of foxtail millet. The results shown that a differentially expressed genes (DEGs) of FATA/B for the synthesis of FA was highly expressed in JG35, and the related genes for the synthesis DG (ACCase, KAS, HAD, KCS, LACS and GAPT), TG (DGAT and PDAT) and CL (CLS) were highly expressed in JG39. The results of this study will provide a theoretical basis for the future study of lipidomics, improvement of lipid quality directionally and breeding of idiosyncratic quality varieties in foxtail millet.

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Leishmania mexicana centrin knockout parasites promote M1-polarizing metabolic changes

Volpedo,

Pacheco-Fernandez,

Oljuskin

et al. 2023

iScience

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Linoleic Acid—A Feasible Preventive Approach for Visceral Leishmaniasis

Cited by 6 publications

References 36 publications

Leishmania mexicana Centrin Knock out Parasites Promote M1-polarizing Metabolic Changes

Leishmania mexicana Centrin Knock out Parasites Promote M1-polarizing Metabolic Changes

Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)

Leishmania mexicana centrin knockout parasites promote M1-polarizing metabolic changes

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