Linolenic acid enhances contraction induced by phenylephrine in isolated rat aorta

Lee, Soo Hee; Kwon, Seong-Chun; Ok, Seong-Ho; Ahn, Seung Hyun; Bae, Sung Il; Hwang, Yeran; Park, Kyeong-Eon; Sohn, Ju-Tae

doi:10.1016/j.ejphar.2020.173662

Cited by 7 publications

(13 citation statements)

References 35 publications

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“…Second, the effects of cellular signaling pathway inhibitors in endothelium-intact thoracic aortas were examined to verify whether theophylline-induced vasodilation was associated with endothelial NO-mediated vasodilation. After endothelium-intact rat thoracic aortas were pretreated with the NOS inhibitor, N W -nitro-L-arginine methyl ester (L-NAME, 10 -4 M); non-specific GC inhibitor, methylene blue (10 -6 M); and NO-sensitive GC inhibitor, 1H- [1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one (ODQ, 10 -5 M) alone for 20 min, phenylephrine (10 -6 M) induced sustained and stable contractions (Lee et al 2021). Theophylline (10 -7 to 3×10 -4 M) was cumulatively added to the organ bath to induce vasodilation in endothelium-intact thoracic aortas with or without the inhibitors (L-NAME, methylene blue, and ODQ).…”

Section: Experimental Protocolsmentioning

confidence: 99%

Theophylline-induced endothelium-dependent vasodilation is mediated by increased nitric oxide release and phosphodiesterase inhibition in rat aorta

Park,

Lee,

Bae

et al. 2023

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This study aimed to examine the endothelial dependence of vasodilation induced by the phosphodiesterase inhibitor theophylline in isolated rat thoracic aortas and elucidate the underlying mechanism, with emphasis on endothelial nitric oxide (NO). The effects of various inhibitors and endothelial denudation on theophylline-induced vasodilation, and the effect of theophylline on vasodilation induced by NO donor sodium nitroprusside, cyclic guanosine monophosphate (cGMP) analog bromo-cGMP, and β-agonist isoproterenol in endotheliumdenuded aorta were examined. The effects of theophylline and sodium nitroprusside on cGMP formation were also examined. We examined the effect of theophylline on endothelial nitric oxide synthase (eNOS) phosphorylation and intracellular calcium levels. Theophylline-induced vasodilation was greater in endothelium-intact aortas than that in endothelium-denuded aortas. The NOS inhibitor, N W -nitro-L-arginine methyl ester; non-specific guanylate cyclase (GC) inhibitor, methylene blue; and NO-sensitive GC inhibitor, 1H-[1,2,4]oxadiazolo [4,3-a] quinoxalin-1-one inhibited theophylline-induced vasodilation in endothelium-intact aortas. Theophylline increased the vasodilation induced by sodium nitroprusside, bromo-cGMP, and isoproterenol. Theophylline increased cGMP formation in endothelium-intact aortas, and sodium nitroprussideinduced cGMP formation in endothelium-denuded aortas. Moreover, theophylline increased stimulatory eNOS (Ser1177) phosphorylation and endothelial calcium levels, but decreased the phosphorylation of inhibitory eNOS (Thr495). These results suggested that theophylline-induced endothelium-dependent vasodilation was mediated by increased endothelial NO release and phosphodiesterase inhibition.

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Section: Experimental Protocolsmentioning

confidence: 99%

Theophylline-induced endothelium-dependent vasodilation is mediated by increased nitric oxide release and phosphodiesterase inhibition in rat aorta

Park,

Lee,

Bae

et al. 2023

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“…Isolated rat aortas were prepared and isometric tension was measured as described previously (Lee et al 2021). Male Sprague-Dawley rats (body weight: 230-280 g; Koatech, Pyeongtaek, Gyeonggi-do, Korea) were euthanized with 100% CO 2 supplied to a small hole in the rat cage.…”

Section: Preparation Of Isolated Rat Aorta and Isometric Tension Meas...mentioning

confidence: 99%

Chloroquine inhibits vasodilation induced by ATP-sensitive potassium channels in isolated rat aorta

Park¹,

Lee²,

Bae³

et al. 2023

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This study examined the effect of chloroquine on vasodilation induced by levcromakalim in isolated endothelium-denuded rat aortas and clarified the underlying mechanisms. We examined the effects of chloroquine, hydroxychloroquine, lipid emulsion, reactive oxygen species (ROS) scavenger N-acetyl-ʟ-cysteine (NAC), and K ATP channel inhibitor glibenclamide on levcromakaliminduced vasodilation. The effects of chloroquine, hydroxychloroquine, NAC, and levcromakalim on membrane hyperpolarization and ROS production were examined in aortic vascular smooth muscle cells (VSMCs). Chloroquine inhibited levcromakalim-induced vasodilation more than hydroxychloroquine. NAC attenuated chloroquine-mediated inhibition of levcromakalim-induced vasodilation, while lipid emulsion had no effect. Glibenclamide eliminated levcromakalim-induced vasodilation in aortas pretreated with chloroquine. Chloroquine and hydroxychloroquine inhibited levcromakalim-induced membrane hyperpolarization in VSMCs. Chloroquine and hydroxychloroquine both produced ROS, but chloroquine produced more. NAC inhibited chloroquine-induced ROS production in VSMCs. Collectively, these results suggest that, partially through ROS production, chloroquine inhibits levcromakalim-induced vasodilation. In addition, chloroquine-induced K ATP channel-induced vasodilation impairment was not restored by lipid emulsion.

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“…Male Sprague-Dawley rats weighing 220-300 g (Koatech, Pyeongtaek, Gyeonggi-do, Korea) were sacrificed using 100% CO 2 supplied to the cage via a small hole. The isolated rat aortae to be obtained from rats were prepared to measure the isometric tension as described previously [31]. The thorax of the rats was exposed, and the descending thoracic aortae were removed from the thoracic cavity.…”

Section: Preparation Of the Isolated Aorta Obtained From Rats And Isometric Tension Measurementmentioning

confidence: 99%

“…4.4. Measurement of cGMP in the Isolated Endothelium-Intact Aorta cGMP measurement was performed as described previously [31]. Acetylation reagent contained in cGMP Complete Kit (Abcam, Cambridge Science Park, Cambridge, England) was used to measure cGMP.…”

Section: Measurement Of Dmt Concentrations In Krebs Solutionmentioning

confidence: 99%

“…Western blot analysis was performed using our previously described method [31]. Briefly, cell lysates were prepared in radioimmunoprecipitation assay buffer (Cell Signaling Technology, Beverly, MA, USA) containing protease inhibitor cocktail (Thermo Fisher Scientific, Rockfield, IL, USA) and phosphatase inhibitor cocktail (Thermo Fisher Scientific).…”

Section: Western Blotmentioning

confidence: 99%

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Lipid Emulsion Enhances Vasoconstriction Induced by Dexmedetomidine in the Isolated Endothelium-Intact Aorta

Lee

Ahn

et al. 2021

IJMS

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This study aimed to examine the effect of lipid emulsion (LE) on the vasoconstriction induced by dexmedetomidine (DMT) in the isolated rat aorta and elucidate the associated cellular mechanism. The effect of LE, NW-nitro-L-arginine methyl ester (L-NAME), and methyl-β-cyclodextrin (MβCD) on the DMT-induced contraction was examined. We investigated the effect of LE on the DMT-induced cyclic guanosine monophosphate (cGMP) formation and DMT concentration. The effect of DMT, LE, 4-Amino-3-(4-chlorophenyl)-1-(t-butyl)-1H-pyrazolo[3,4-d]pyrimidine,4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), and rauwolscine on the phosphorylation of endothelial nitric oxide synthase (eNOS), caveolin-1, and Src kinase was examined in the human umbilical vein endothelial cells. L-NAME, MβCD, and LE (1%, standardized mean difference (SMD): 2.517) increased the DMT-induced contraction in the endothelium-intact rat aorta. LE (1%) decreased the DMT (10−6 M) concentration (SMD: −6.795) and DMT-induced cGMP formation (SMD: −2.132). LE (1%) reversed the DMT-induced eNOS (Ser1177 and Thr496) phosphorylation. PP2 inhibited caveolin-1 and eNOS phosphorylation induced by DMT. DMT increased the Src kinase phosphorylation. Thus, LE (1%) enhanced the DMT-induced contraction by inhibition of NO synthesis, which may be caused by the decreased DMT concentration. DMT-induced NO synthesis may be caused by the increased eNOS (Ser1177) phosphorylation and decreased eNOS (Thr495) phosphorylation potentially mediated by Src kinase-induced caveolin-1 phosphorylation.

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Linolenic acid enhances contraction induced by phenylephrine in isolated rat aorta

Cited by 7 publications

References 35 publications

Theophylline-induced endothelium-dependent vasodilation is mediated by increased nitric oxide release and phosphodiesterase inhibition in rat aorta

Theophylline-induced endothelium-dependent vasodilation is mediated by increased nitric oxide release and phosphodiesterase inhibition in rat aorta

Chloroquine inhibits vasodilation induced by ATP-sensitive potassium channels in isolated rat aorta

Lipid Emulsion Enhances Vasoconstriction Induced by Dexmedetomidine in the Isolated Endothelium-Intact Aorta

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