Lipase-catalyzed Hydrolysis of Linseed Oil : Optimization Using Response Surface Methodology

Chen, Weiwei; Sun, Shangde; Liang, Shuai; Peng, Lincai; Wang, Yadong; Mi, Shen

doi:10.5650/jos.ess13189

Cited by 24 publications

(21 citation statements)

References 24 publications

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“…These results showed that enough water is necessary for the hydrolysis of Phoenix tree seed oil. Similar effect of water on the lipase-catalyzed hydrolysis of oil can also be found in previous reports (Chen et al, 2014;Prado and Saldaña, 2013).…”

Section: Effect Of Mass Ratio Of Water To Oilsupporting

confidence: 88%

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Hou

Sun

2017

Grasas y Aceites

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SUMMARY:Phoenix tree (Firmiana simplex) seed oil is a novel oil which is rich in sterculic acid. Sterculic acid, a cyclopropene fatty acid, can be used as the inhibitor of the stearoyl-CoA desaturase system and mammary carcinomas growth. In this work, Lipozyme TLIM-catalyzed hydrolysis of the novel Phoenix tree seed oil was used to prepare sterculic acid. High temperature GC-FID and the degree of hydrolysis (DH) were used to monitor the reaction progress. Effects of reaction variables on the hydrolysis were evaluated and optimized using response surface methodology. Results showed that sterculic acid can be successfully prepared from the novel seed oil, and the effect of reaction variables on the hydrolysis decreased in the order of reaction time > enzyme load > temperature. RESUMEN: Producción enzimática de ácido estercúlico a partir del nuevo aceite de semillas del árbol fenix: optimización y estudio cinético. El aceite de semilla del árbol fenix (Firmiana simplex) es un nuevo tipo de aceite rico en ácido estercúlico. Este es un ácido graso ciclopropeno, que se puede utilizar como inhibidor del sistema de estearoyl-CoA desaturasa y del crecimiento de los carcinomas mamarios. En este trabajo, se utilizó la hidrólisis catalizada por Lipozyme TLIM del nuevo aceite de semilla de árbol fenix para preparar ácido estercúlico. Se utilizaron GC-FID a alta temperatura y el grado de hidrólisis (DH) para monitorizar el progreso de la reacción. Los efectos de las variables de reacción de hidrólisis se evaluaron y optimizaron utilizando la metodología de superficie de respuesta. Los resultados mostraron que el ácido estercúlico puede prepararse con éxito a partir del nuevo aceite de semilla, y el efecto de las variables de reacción en la hidrólisis disminuyó en el orden de tiempo de reacción> carga enzimática> temperatura. Se pueden obtener altos rendimientos de ácidos grasos (DH, 98,2 ± 0,8%) en condiciones optimizadas (45 ºC, relación masa de agua a aceite 10: 1, carga enzimática 10% y 18 h). La ecuación de Arrhenius para la hidrólisis fue LnV 0 = 9,12 − 4721/T. La energía de activación fue 39,25KJ/mol. Los valores cinéticos de Vmax, K/m fueron 0,232 mol/(L • min) y 0,084 mol/L, respectivamente. Citation/Cómo citar este artículo: Hou X, Sun S. 2017. Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study. Grasas Aceites 68 (2), e197. http://dx

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Section: Effect Of Mass Ratio Of Water To Oilsupporting

confidence: 88%

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Hou

Sun

2017

Grasas y Aceites

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“…A Box–Behnken experimental design [27,28] was chosen to assess the relationship between the yield of biodiesel and the reactant ratio, amount of IL and time. A total of 17 sets of experiments (12 factorial points and five centre points) were performed according to a 3 3 Box–Behnken experimental design.…”

Section: Methodsmentioning

confidence: 99%

Optimization and kinetic study of methyl laurate synthesis using ionic liquid [Hnmp]HSO ₄ as a catalyst

et al. 2018

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Methyl laurate was synthesized from lauric acid (LA) and methanol via an esterification reaction using ionic liquids (ILs) as catalysts. The efficiencies of three different catalysts, 1-methylimidazole hydrogen sulfate ([Hmim]HSO4), 1-methyl-2-pyrrolidonium hydrogen sulfate ([Hnmp]HSO4) and H2SO4, were compared. The effect of the methanol/LA molar ratio, reaction temperature, reaction time and catalyst dosage on the esterification rate of LA was investigated by single-factor experiments. Based on the single-factor experiments, the esterification of LA and methanol was optimized using response surface methodology. The results showed that the most effective catalyst was the IL [Hnmp]HSO4. The optimal conditions were as follows: [Hnmp]HSO4 dosage of 5.23%, methanol/LA molar ratio of 7.68 : 1, reaction time of 2.27 h and reaction temperature of 70°C. Under these conditions, the LA conversion of the esterification reached 98.58%. A kinetic study indicated that the esterification was a second-order reaction with an activation energy and a frequency factor of 68.45 kJ mol−1 and 1.9189 × 109 min−1, respectively. The catalytic activity of [Hnmp]HSO4 remained high after five cycles.

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“…To enhance the hydrolysis efficiency, Ting et al immobilized lipase derived from C. rugosa to chitosan beads and used the enzyme for soybean oil hydrolysis. High hydrolysis yield (<95%) was achieved for a reaction time of 25 h. Recently, Chen et al compared the hydrolysis of linseed oil by using various immobilized lipases such as lipase AY, Liopzyme RM‐IM, Lipozyme TLIM, and Novozyme 435. Among those enzymes, immobilized lipase AY displayed the highest efficiency with hydrolysis yield of 93.92% for a reaction time of 3.33 h .…”

Section: Resultsmentioning

confidence: 99%

“…High hydrolysis yield (<95%) was achieved for a reaction time of 25 h. Recently, Chen et al compared the hydrolysis of linseed oil by using various immobilized lipases such as lipase AY, Liopzyme RM‐IM, Lipozyme TLIM, and Novozyme 435. Among those enzymes, immobilized lipase AY displayed the highest efficiency with hydrolysis yield of 93.92% for a reaction time of 3.33 h . A combination of immobilized lipases for oil hydrolysis was also reported by Alves et al The combination of Lipozyme RM‐IM and Novozyme 435 obtained hydrolysis yield of 80% for a reaction time of 24 h. In the present study, Eversa Transform lipase obtained high hydrolysis yield (94.16%) for a short reaction time (8.41 h).…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, immobilized lipases have been extensively developed for hydrolysis reactions to improve the stability and reusability of lipase . However, the rate of the reaction catalyzed by immobilized lipase is lower than that using other lipases because of the mass transfer resistance caused by two immiscible phases of the substrates and heterogeneous biocatalyst .…”

Section: Introductionmentioning

confidence: 99%

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Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Nguyen

et al. 2018

Biotechnology Progress

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Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Transform lipase, a new and low‐cost liquid lipase formulation, was used for the first time in oil hydrolysis with gac oil as a triglyceride source in this study. Response surface methodology was employed to optimize the reaction conditions and establish a reliable mathematical model for predicting hydrolysis yield. A maximal yield of 94.16% was obtained at a water‐to‐oil molar ratio of 12.79:1, reaction temperature of 38.9 °C, enzyme loading of 13.88%, and reaction time of 8.41 h. Under this optimal reaction condition, Eversa Transform lipase could be reused for up to eight cycles without significant loss in enzyme activity. This study indicates that the use of liquid Eversa Transform lipase in enzyme‐catalyzed oil hydrolysis could be a promising and cheap method of fatty acid production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018

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Lipase-catalyzed Hydrolysis of Linseed Oil : Optimization Using Response Surface Methodology

Cited by 24 publications

References 24 publications

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Optimization and kinetic study of methyl laurate synthesis using ionic liquid [Hnmp]HSO ₄ as a catalyst

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

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Lipase-catalyzed Hydrolysis of Linseed Oil : Optimization Using Response Surface Methodology

Cited by 24 publications

References 24 publications

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Optimization and kinetic study of methyl laurate synthesis using ionic liquid [Hnmp]HSO 4 as a catalyst

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

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Optimization and kinetic study of methyl laurate synthesis using ionic liquid [Hnmp]HSO ₄ as a catalyst