Lipase-catalyzed process for biodiesel production: Enzyme immobilization, process simulation and optimization

Zhao, Xuebing; Qi, Feng; Yuan, Ching; Du, Wei; Liu, Dehua

doi:10.1016/j.rser.2014.12.021

Cited by 331 publications

(178 citation statements)

References 118 publications

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“…According to other studies, the equilibrium of reactions can be affected by the temperature of the reaction. [15,36] In general, elevating the reaction temperature increases the percentage conversion, as the equilibrium constant was also increased. Apart from the improvement in mass transfer limitation between reactant and catalyst, the solubility of the reaction mixture is increased [49] and the viscosity of the medium is reduced, therefore, enhancing proper mixing of the reactants.…”

Section: Rsm Experiments and Model Fittingmentioning

confidence: 99%

“…[14] In this context, the simpler and more economical method of immobilization is the physical attachment of CRL onto multi-walled carbon nanotubes (MWCNTs) as a support. This approach is suited for the fitting of large amounts of lipases, allowing large-scale processes [15] and offering a feasible solution for the extension of the reaction life of the lipases. [16] Such an extended reaction life may be accredited to the excellent binding capacity of the MWCNTs due to their large surface area to volume ratio, interesting physicochemical properties and biological compatibility.…”

Section: Introductionmentioning

confidence: 99%

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Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Marzuki

Huyop

Aboul‐Enein

et al. 2015

Biotechnology & Biotechnological Equipment

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Esters, such as methyl oleate, are functionally important compounds in many industrial sectors, mainly as components for manufacturing of emulsifiers, detergents, intermediate stabilizers and wetting agents. Acid functionalization of multiwalled carbon nanotubes (F-MWCNTs) by using a mixture of HNO 3 and H 2 SO 4 (1:3, v:v) was employed as the matrix for the adsorption of Candida rugosa lipase (CRL) as nanobioconjugates (CRL-MWCNTs) for the production of methyl oleate. Structural information of the developed CRL-MWCNTs was confirmed using Fourier Transform Infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy, which revealed a successful attachment of CRL onto the F-MWCNTs. Process parameters (reaction time, temperature and alcohol:acid molar ratio) were optimized for high percent conversion of methyl oleate. Structural analysis established that CRL was successfully attached to the surface of the F-MWCNTs. Under the optimized conditions, which were 13.87 h, 51 C, molar ratio oleic acid: methanol (1:3.80), a high ester yield of 90.90% was attained. Also, under conditions of the shortest reaction time of 6 h, 47.07 C and acid:methanol ratio of 1:2.54, the CRL-MWCNTs catalysed a 74.51% ester yield. Hence, we established that response surface methodology (RSM) can be a practical technique for the prediction of the conditions that favour the high yield production of methyl oleate. Under optimized conditions, the CRL-MWCNTs nanobioconjugates are potentially good and economical biocatalysts for the production of methyl oleate, as well as other types of commercially important fatty-acid methyl esters.

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Section: Rsm Experiments and Model Fittingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Marzuki

Huyop

Aboul‐Enein

et al. 2015

Biotechnology & Biotechnological Equipment

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“…Therefore, reuse of immobilized lipase had a significant effect on biodiesel production. For example, when lipase price are 1500, 1000, 750, 200, and 100 $/kg, respectively, the immobilized lipase needs to be reused for more than 320, 210, 160, 50, and 20 batch reactions without significant loss of catalytic activity [61]. Sotoft et al [62] also investigated TEA of enzymatic biodiesel production.…”

Section: Techno-economic and Life Cycle Analysis Of Enzymatic Biodiesmentioning

confidence: 99%

Environmentally-Benign Dimethyl Carbonate-Mediated Production of Chemicals and Biofuels from Renewable Bio-Oil

Kim

Lee

2017

Energies

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Due to the increasing emission of carbon dioxide (CO 2 ), the development of fuels and chemicals based on renewable resources has attracted much attention. Bio-oil, as a carbon rich material, has been considered as a feedstock for biodiesel production. In conventional methanol-mediated transesterification of bio-oil for biodiesel production, significant amounts of glycerol are being generated as a byproduct. In order to overcome these issues, dimethyl carbonate (DMC) has been recently used as an alternative acyl acceptor to avoid the generation of glycerol. DMC is an environmentally-benign chemical reagent and reactive solvent due to safety, health, and environmental benefits. Moreover, DMC can be produced from CO 2 . Co-production of biodiesel and chemicals such as glycerol carbonate is possible as the concept of zero-waste utilization of bio-oil. Value-added chemicals can be synthesized using DMC as a reagent. This paper provides a review on the physical and chemical properties of DMC as a solvent, as well as the production methods for DMC. DMC-mediated production of various chemicals and fuels in both chemical and enzymatic processes are discussed together with their pros and cons.

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“…Enzyme immobilization is a well-reported technology that allows application of enzymes in many biocatalyzed processes like in lipase-mediated biodiesel production [9,22,40]. In general, immobilization allows reuse of the biocatalyst, makes the product recovery easier, and frequently enhances enzyme resistance against inactivation by different denaturants, providing more stable and efficient catalysts [41,42].…”

Section: Lipase Immobilizationmentioning

confidence: 99%

Moving towards a Competitive Fully Enzymatic Biodiesel Process

et al. 2015

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Enzymatic biodiesel synthesis can solve several problems posed by the alkaline-catalyzed transesterification but it has the drawback of being too expensive to be considered competitive. Costs can be reduced by lipase improvement, use of unrefined oils, evaluation of soluble/immobilized lipase preparations, and by combination of phospholipases with a soluble lipase for biodiesel production in a single step. As shown here, convenient natural tools have been developed that allow synthesis of high quality FAMEs (EN14214) from unrefined oils in a completely enzymatic single-step process, making it fully competitive.

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Lipase-catalyzed process for biodiesel production: Enzyme immobilization, process simulation and optimization

Cited by 331 publications

References 118 publications

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Environmentally-Benign Dimethyl Carbonate-Mediated Production of Chemicals and Biofuels from Renewable Bio-Oil

Moving towards a Competitive Fully Enzymatic Biodiesel Process

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