2021
DOI: 10.1002/bab.2141
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Lipase immobilization on biodegradable film with sericin

Abstract: An ecofriendly and low‐cost film composed by cassava starch, polyvinyl alcohol, and sericin blend (CS–PVA–SS) was synthesized, characterized, and applied as a novel support for Botryosphaeria ribis EC‐01 lipase immobilization by enzyme–film–enzyme adsorption. Film revealed thickness between 230 and 309 μm and higher flexibility and malleability in comparison with film without SS. Based on p‐nitrophenyl palmitate hydrolysis reaction, the activity retention of immobilized lipase was 987%. For optimal conditions,… Show more

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Cited by 5 publications
(3 citation statements)
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“…The maximum yield was obtained at 49 • C, molar ratio oleic acid: Ethanol of 1:3, 1.25 g lipase film or 50 U (1.03 ± 0.03 mg protein), and 30 h of reaction. Even after seven use cycles, immobilized lipase showed a 52% reduction in ester yield [53].…”
Section: Immobilization On Different Pva Hybrid Supportsmentioning
confidence: 98%
See 1 more Smart Citation
“…The maximum yield was obtained at 49 • C, molar ratio oleic acid: Ethanol of 1:3, 1.25 g lipase film or 50 U (1.03 ± 0.03 mg protein), and 30 h of reaction. Even after seven use cycles, immobilized lipase showed a 52% reduction in ester yield [53].…”
Section: Immobilization On Different Pva Hybrid Supportsmentioning
confidence: 98%
“…The molecular weight of SS ranges from 24 to 400 kDa,12 and serine (32.3%),13 aspartic acid (16.71%), and glycine (13.49%) are the primary amino acids. This biomaterial enriches polymer mixtures reducing environmental damage due to its degradability [53]. This material, in combination with cassava starch and PVA, has been used to immobilize Botryosphaeria ribis (Br) lipase using a sandwich adsorption methodology.…”
Section: Immobilization On Different Pva Hybrid Supportsmentioning
confidence: 99%
“…16,17 CALB is very special, having a peculiar mechanism of action. Compared with other lipases, 18,19 CALB does not have a "lid" structure and has a wider substrate selectivity. The catalysis of CALB follows the Ping Pong Bi-Bi mechanism, during which the enzyme goes through the process of free enzyme-transition state 1-acylase intermediatetransition state 2-free enzyme.…”
Section: Introductionmentioning
confidence: 99%