Lipase immobilization on Polysulfone globules and their performances in olive oil hydrolysis

Gupta, Shweta; Singh, Kunwar P.; Bhattacharya, Amit

doi:10.1016/j.ijbiomac.2010.02.001

Cited by 16 publications

(4 citation statements)

References 39 publications

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“…This result indicated that the catalytic performance of lipase was affected by the immobilization method, and immobilization method protected the enzyme activity. A similar phenomenon has been reported previously 22 . Where a lipase from Candida rugosa immobilized on chitosan beads exhibited better pH stability as compared to soluble lipase 23 .…”

Section: Effect Of Buffer Ph On the Activity Of Free And Immobilized supporting

confidence: 91%

Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

2014

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Section: Effect Of Buffer Ph On the Activity Of Free And Immobilized supporting

confidence: 91%

Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

2014

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“…In organic medium, lipases also catalyze esterifications, transesterifications and interesterifications [1][2][3][4]. These enzymes are applied in several industrial processes including synthesis of biopolymers, production of pharmaceuticals, agrochemicals, cosmetics, flavors, treatment of waste rich in lipids and biodiesel synthesis by transesterification of triglycerides with short-chain alcohols [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immobilized lipases on poly-hydroxybutyrate beads to catalyze biodiesel synthesis

Mendes

Oliveira

Velez

et al. 2012

International Journal of Biological Macromolecules

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Five microbial lipase preparations from several sources were immobilized by hydrophobic adsorption on small or large poly-hydroxybutyrate (PHB) beads and the effect of the support particle size on the biocatalyst activity was assessed in the hydrolysis of olive oil, esterification of butyric acid with butanol and transesterification of babassu oil (Orbignya sp.) with ethanol. The catalytic activity of the immobilized lipases in both olive oil hydrolysis and biodiesel synthesis was influenced by the particle size of PHB and lipase source. In the esterification reaction such influence was not observed. Geobacillus thermocatenulatus lipase (BTL2) was considered to be inadequate to catalyze biodiesel synthesis, but displayed high esterification activity. Butyl butyrate synthesis catalyzed by BTL2 immobilized on small PHB beads gave the highest yield (≈90 mmol L(-1)). In biodiesel synthesis, the catalytic activity of the immobilized lipases was significantly increased in comparison to the free lipases. Full conversion of babassu oil into ethyl esters was achieved at 72 h in the presence of Pseudozyma antarctica type B (CALB), Thermomyces lanuginosus lipase (Lipex(®) 100 L) immobilized on either small or large PHB beads and Pseudomonas fluorescens (PFL) immobilized on large PHB beads. The latter preparation presented the highest productivity (40.9 mg of ethyl esters mg(-1) immobilized protein h(-1)).

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“…The Micahaelis-Menten equation 42,43 fit the linear Lineweaver-Burk transformation, and it is as follows…”

Section: Resultsmentioning

confidence: 99%

Comparative study of the hydrolysis of different oils by lipase‐immobilized membranes

Gupta

Ingole

Singh

et al. 2011

J of Applied Polymer Sci

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Lipase immobilization on Polysulfone globules and their performances in olive oil hydrolysis

Cited by 16 publications

References 39 publications

Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

Characterization and Catalytic Properties of Free and Silica-Bound Lipase: a Comparative Study

Evaluation of immobilized lipases on poly-hydroxybutyrate beads to catalyze biodiesel synthesis

Comparative study of the hydrolysis of different oils by lipase‐immobilized membranes

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