Lipase Immobilized onto Metal‐Organic Frameworks for Enantioselective Resolution of Mandelic Acid

Abstract

An immobilized lipase with metal‐organic frameworks (MOFs) was first used for the kinetic resolution of mandelic acid enantiomers by transesterification reaction using vinyl acetate as acyl donor in organic medium. Notably, the biocompatibility of the carrier and catalytic performance of lipase could be significantly improved via modification of NH2‐Ni‐MOF with polyvinylpyrrolidone. After extensively screening the enzymatic process, the optimal enantioselective resolution conditions and excellent outcomes were obtained for the enzyme‐catalyzed transesterification reaction. In addition, the recovered immobilized lipase displayed outstanding reusability after immobilization.

Recent trends in metal‐organic frameworks mediated lipase immobilization: A state‐of‐the‐art review

“…Immobilizing enzymes within MOFs has the potential to greatly increase their stability and, in turn, their reusability (Liang et al., 2020; Nadar & Rathod, 2020). A polyvinylpyrrolidone (PVP)‐modified NH 2 ‐Ni‐MOF was produced and employed as a carrier for the immobilization of P. fluorescens lipase (PFL), and the reusability of the immobilized lipase (PFL@NH 2 ‐Ni‐MOF) was studied (Fan et al., 2023). PFL@NH 2 ‐Ni‐MOF was found to retain 50.18% of its original activity after 10 cycles (Fan et al., 2023).…”

“…A polyvinylpyrrolidone (PVP)‐modified NH 2 ‐Ni‐MOF was produced and employed as a carrier for the immobilization of P. fluorescens lipase (PFL), and the reusability of the immobilized lipase (PFL@NH 2 ‐Ni‐MOF) was studied (Fan et al., 2023). PFL@NH 2 ‐Ni‐MOF was found to retain 50.18% of its original activity after 10 cycles (Fan et al., 2023). Zhong et al.…”

Efficient resolution of 4‐chlormandelic acid enantiomers using lipase@UiO‐67(Zr) zirconium–organic frameworks in organic solvent

“…The progress has been achieved in the area of chiral resolution employ lipase immobilization on MOF materials. [22][23][24][25][26][27][28][29][30][31] It can be meet the basic requirements of enzyme immobilization.…”

“…In recent years, the development of MOF materials with high surface area and pore volume, pore size can be adjusted, and materials surface can be modified. The progress has been achieved in the area of chiral resolution employ lipase immobilization on MOF materials 22–31 . It can be meet the basic requirements of enzyme immobilization.…”

Enzyme-immobilized spherical covalent organic frameworks as nanoreactors for heterogeneous biocatalysis

“…[26][27][28] In addition, some additives, such as polyvinylpyrrolidone (PVP), are still required in the synthetic process of enzyme/MOF composites. 29,30 Hence, it is an urgent need to construct novel porous materials with high stability and large pores to efficiently immobilize enzymes.…”