Lipid analysis and lipidomics investigation by ambient mass spectrometry

Deng, Jiewei; Yang, Yunyun; Luo, Lijuan; Xiao, Yipo; Luan, Tiangang

doi:10.1016/j.trac.2020.115924

Cited by 17 publications

(8 citation statements)

References 113 publications

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“…Not only are these protocols time consuming and labor intensive, but they also preclude the use of the sample for additional tests, as it has been permanently modified [ 9 ]. To avoid tissue disintegration, researchers have worked to develop new approaches that do not require sample homogenization, such as ambient mass-spectrometry methods (e.g., desorption electrospray ionization mass spectrometry (DESI-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)) [ 10 ]. However, such analytical methods still require tissue to be excised and cut into thin slices.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Bogusiewicz

Kupcewicz

Goryńska

et al. 2022

IJMS

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The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method of enabling the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology.

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Section: Introductionmentioning

confidence: 99%

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Bogusiewicz

Kupcewicz

Goryńska

et al. 2022

IJMS

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“…), on many different platforms, and the data analyzed in many different ways. Complete details on these workflows are beyond the scope of the current discussion and readers are directed to multiple excellent resources on these topics. ,− This Perspective will instead focus on emerging technologies that enable accurate lipid structural elucidation.…”

Section: Lipid Analysis By Mass Spectrometrymentioning

confidence: 99%

Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation

Bonney

Prentice

2021

Anal. Chem.

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Lipids and metabolites are of interest in many clinical and research settings because it is the metabolome that is increasingly recognized as a more dynamic and sensitive molecular measure of phenotype. The enormous diversity of lipid structures and the importance of biological structure–function relationships in a wide variety of applications makes accurate identification a challenging yet crucial area of research in the lipid community. Indeed, subtle differences in the chemical structures of lipids can have important implications in cellular metabolism and many disease pathologies. The speed, sensitivity, and molecular specificity afforded by modern mass spectrometry has led to its widespread adoption in the field of lipidomics on many different instrument platforms and experimental workflows. However, unambiguous and complete structural identification of lipids by mass spectrometry remains challenging. Increasingly sophisticated tandem mass spectrometry (MS/MS) approaches are now being developed and seamlessly integrated into lipidomics workflows to meet this challenge. These approaches generally either (i) alter the type of ion that is interrogated or (ii) alter the dissociation method in order to improve the structural information obtained from the MS/MS experiment. In this Perspective, we highlight recent advances in both ion type alteration and ion dissociation methods for lipid identification by mass spectrometry. This discussion is aimed to engage investigators involved in fundamental ion chemistry and technology developments as well as practitioners of lipidomics and its many applications. The rapid rate of technology development in recent years has accelerated and strengthened the ties between these two research communities. We identify the common characteristics and practical figures of merit of these emerging approaches and discuss ways these may catalyze future directions of lipid structural elucidation research.

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“…The phospholipid bilayer and associated lipids provide a permeability barrier and a structured environment that is essential for membrane-bound proteins to perform their proper role (63). Lipidomic analyses provide a suitable tool for elucidating the specific roles of lipid intermediates in cell signaling, so more in-depth knowledge of the complexity of lipid signaling will increase our understanding of the role of lipid metabolism in various CNS disorders and open new opportunities for the development of drugs and therapies designed to treat neurological diseases (64). Lipid carriers such as SLN prepared with Compritol ® 888 ATO revealed that the proportion of lipid content plays an essential role in modulating excitability in the brain.…”

Section: Orally-administered Solid Lipid Nanoparticles (Sln) For Brain Drug Deliverymentioning

confidence: 99%

Solid Lipid Nanoparticles: An Approach to Improve Oral Drug Delivery

et al. 2021

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Nanoparticles have shown overall beneficial effects in drug administration. Specifically, solid lipid nanoparticles (SLN) have emerged as an alternative to polymer-based systems. However, the oral administration of SLN, the first choice for conventional medications, has not been addressed due to the taboo surrounding the complicated transit that this delivery route entails. This review focuses on the encapsulation of drugs into SLN as a strategy for improving oral administration. Examples of applications of SLN to enhance the absorption and bioavailability of poorly-soluble drugs and protect acid-labile active molecules are discussed. This work also emphasizes the importance of developing SLN-based systems to treat health issues such as neurological diseases and cancer, and combat antibiotic resistance, three significant and increasingly common current public health problems. The review sections clarify how SLN can improve bioavailability, target therapeutic agents, and reduce side effects.

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Lipid analysis and lipidomics investigation by ambient mass spectrometry

Cited by 17 publications

References 113 publications

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation

Solid Lipid Nanoparticles: An Approach to Improve Oral Drug Delivery

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