Lipid associated tissue ferritin

Cham, Bill E.; Roeser, Peter; Nikles, A.; Ridgway, Kathleen M.

doi:10.1016/0009-8981(86)90117-8

Cited by 6 publications

(11 citation statements)

References 10 publications

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“…This may be due to the purification process. The high yield we have obtained may be due to the lower loss of protein achieved by our procedure since multiple steps used in the isolation and purification of ferritin cause high losses of the protein (Frenkel et al ., 1983;Cham et al ., 1985Cham et al ., , 1986.…”

Section: Discussionmentioning

confidence: 97%

Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs

et al. 2005

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Ferritin is the iron-storage protein responsible for sequestering excess iron, to be stored in a safe way in the liver or to be shed with the intestinal epithelial cells. The properties of ferritin in iron-overload-susceptible birds have not been elucidated. Furthermore, there is only scarce information on mucosal ferritin, with no information at all in avian species. Here we have studied the liver and proximal intestine ferritins of iron-overload-susceptible (Indian hill mynahs, common mynahs) and non-susceptible (turtledoves, chicken) bird species. A brief purification process preceded native polyacrylamide gel electrophoresis and staining the gels for protein and iron. Protein amounts and iron-binding characteristics of ferritin were measured and ferritin saturation levels were calculated. Although ferritin protein amounts did not differ significantly, liver and mucosal ferritins of sensitive bird species incorporated much more iron, leading to high saturation levels. Significantly higher ferritin iron content and saturation were observed in the liver of both mynah species and in the intestinal ferritin of Indian hill mynahs when compared with the non-susceptible species. Ferritin appears not to play a major role in the regulation of iron absorption, implicating other phases in iron transport to be more important in the onset and process of iron overload in birds.

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Section: Discussionmentioning

confidence: 97%

Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs

et al. 2005

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“…It has since been shown that a significant proportion of ferritin is present as non-soluble protein in the liver of various mammals (Cham et al, , 1988(Cham et al, , 1989). This ferritin can be solubilized by lipid solvent systems (Cham et al, 1986) or by non-ionic detergents (Cham et al, 1988). The newly described ferritin pool has therefore been termed lipid-associated ferritin (LAF) (Cham et al, , 1988(Cham et al, , 1989.…”

Section: Introductionmentioning

confidence: 99%

“…The newly described ferritin pool has therefore been termed lipid-associated ferritin (LAF) (Cham et al, , 1988(Cham et al, , 1989. The difference between cytosolic ferritin (CYF) and LAF is not only physicochemical (Cham et al, 1986(Cham et al, , 1988 but also metabolic (Cham et al, 1989). At homoeostasis the rate of turnover of CYF is much higher than that of LAF. Ascorbic acid is involved in a large number of cellular metabolic events.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic acid deficiency affects the metabolism of cytosolic ferritin but not lipid-associated ferritin in livers of guinea pigs

Cham

Roeser

Nikles

1990

Biochemical Journal

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Two physicochemically and metabolically separate pools of ferritin, namely cytosolic ferritin and lipid-associated ferritin, are present in the livers of guinea pigs. In this paper we establish that the iron content of cytosolic ferritin is dependent on and linearly related to ascorbic acid concentration, whereas changes in concentration of this vitamin do not affect the iron content of lipid-associated ferritin. In livers of ascorbic acid-deficient guinea pigs both synthesis and degradation of cytosolic ferritin are diminished equally. Consequently cytosolic ferritin is metabolized more slowly without changes in its pool size. In contrast with cytosolic ferritin, the metabolism of lipid-associated ferritin is unaffected by ascorbic acid deficiency. The differential effects of ascorbic acid deficiency on the physicochemical characteristics as well as on the metabolism of cytosolic ferritin and lipid-associated ferritin suggest that the two forms of ferritin have different functional roles.

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“…More recently, a further pool of intracellular ferritin has been identified. This ferritin can be made available to antibody by extraction of liver homogenates with an organic solvent system such as butanol/di-isopropyl ether (Cham & Knowles, 1976;Cham et al, 1986). The non-ionic detergents Triton X-100 and Nonidet P-40 will also release this ferritin from lipid, but it cannot be made available to antibody by treatment with 1 00 sodium deoxycholate or by heating to 75°C (Cham et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Thus this ferritin differs from the membrane-associated ferritin described by Sargent & Munro (1975), and it has been called lipid-associated ferritin (LAF). In the liver LAF represents 24-33 00 of total cellular ferritin, depending on the species of mammal (Cham et al, 1986). The composition of LAF (ferritin + lipid) is not known.…”

Section: Introductionmentioning

confidence: 99%

Cytosolic ferritin and lipid-associated ferritin are metabolically different in guinea-pig livers

Cham

Roeser

Nikles

1989

Biochemical Journal

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A distinct pool of liver ferritin has been described in man, guinea pigs and rats [Cham, Roeser, Nikles & Ridgway (1986) Clin. Chim. Acta 158, 71-79]. This ferritin accounts for approx. 30% of total intracellular ferritin. It differs from previously described cytosolic and 'microsomal-fraction' ferritin by its firm association with lipid and by the absence of heat-stability at 75 degrees C. The present study demonstrates that cytosolic ferritin and lipid-associated ferritin in guinea-pig livers have distinctly different rates of turnover. Cytosolic ferritin has a rate of turnover approx. 3.5 times as high as lipid-associated ferritin. The apparent metabolic heterogeneity suggests that the two forms of ferritin may have different functional roles.

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Lipid associated tissue ferritin

Cited by 6 publications

References 10 publications

Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs

Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs

Ascorbic acid deficiency affects the metabolism of cytosolic ferritin but not lipid-associated ferritin in livers of guinea pigs

Cytosolic ferritin and lipid-associated ferritin are metabolically different in guinea-pig livers

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