Lipid composition of the marine dinoflagellate Scrippsiella trochoidea

Harvey, H. Rodger; Bradshaw, Stuart A.; Ohara, Susumu; Eglinton, G.; Corner, E. D. S.

doi:10.1016/0031-9422(88)80432-1

Cited by 73 publications

(77 citation statements)

References 37 publications

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“…CS-295/c. The presence of compound 13 as a dominant sterol appears to be limited mainly to a selection of dinoflagellate genera, including Gymnodinium, Gyrodinium, Heterocapsa, Pfiesteria, Prorocentrum, and Scrippsiella (Harvey et al, 1988;Piretti et al, 1997;Mansour et al, 1999;Leblond & Chapman, 2002. Compound 12 has also been found as a dominant sterol among these genera (Nichols et al, 1984;Mansour et al, 1999;Leblond & Chapman, 2002).…”

Section: Discussionmentioning

confidence: 99%

Fatty acid and sterol composition of two evolutionarily closely related dinoflagellate morphospecies from cold Scandinavian brackish and freshwaters

Leblond¹,

Anderson²,

Kofink³

et al. 2006

European Journal of Phycology

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Little is known about the lipid composition of cold-adapted dinoflagellates. Here, we investigate Peridinium aciculiferum and Scrippsiella hangoei, two cold-adapted dinoflagellate morphospecies that present significant differences in general morphology, physiology and habitat, but which share identical ribosomal DNA (rDNA) sequences, indicating a recent evolutionary divergence between them. Despite their present taxonomic names, it is now known that these two genetically isolated morphospecies are evolutionarily related to the so-called Pfiesteria-like species. We have found that chloroplastassociated glycolipids from P. aciculiferum and S. hangoei contained a higher relative percentage of two polyunsaturated C 18 fatty acids (octadecapentaenoic [18:5(n-3)] and octadecatetraenoic [18:4(n-3)] acids), and a lower relative percentage of hexadecanoic acid (16:0) than typically observed in previously examined warm-adapted dinoflagellate species. This suggests that these two fatty acids provided the requisite membrane fluidity for chloroplasts under a cold growth condition in these two organisms. A similar increase in 18:5(n-3) and 18:4(n-3) over warm-adapted species was also found in the phospholipids and triglycerides. P. aciculiferum and S. hangoei were also examined for the production of unique sterols that may serve as chemical biomarkers. Both were found to have a very similar sterol composition, consisting of two common dinoflagellate sterols, cholestanol and dinostanol, as dominant sterols, along with a number of other common dinoflagellate sterols as minor sterols. This demonstrated the absence of a specific steroidal biomarker but, more importantly, showed that these two morphospecies still produce very similar sterols despite having diverged in several other traits.

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Section: Discussionmentioning

confidence: 99%

Fatty acid and sterol composition of two evolutionarily closely related dinoflagellate morphospecies from cold Scandinavian brackish and freshwaters

Leblond¹,

Anderson²,

Kofink³

et al. 2006

European Journal of Phycology

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“…PUFAs in the marine environment are usually thought to derive mainly from phytoplankton (e.g., Parrish et al 2000), although they are often found in other marine organisms, including herbivorous zooplankton (Corner et al 1986 and references therein) and deep-sea bacteria (DeLong and Yayanos 1985;Jøstensen and Landfald 1997;Fang et al 2000). The main source of PUFAs in the phytoplankton are diatoms which biosynthesise mostly C 20:5 (Volkman et al 1989), and dinoflagellates which produce more C 22:6 (Sargent et al 1987;Harvey et al 1988).…”

Section: Total Lipidsmentioning

confidence: 99%

Lipids and nitrogen isotopes of two deep-water corals from the North-East Atlantic: initial results and implications for their nutrition

Kiriakoulakis

Fisher

Wolff

et al. 2005

Cold-Water Corals and Ecosystems

100

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Abstract. The lipid and organic nitrogen isotopic (δ 15 N) compositions of two common deep-water corals (Lophelia pertusa and Madrepora oculata) collected from selected locations of the NE Atlantic are compared to the composition of suspended particulate organic matter, in order to determine their principle food source. Initial results suggest that they may feed primarily on zooplankton. This is based on the increased abundances of mono-unsaturated fatty acids and alcohols and the different ratios of the polyunsaturated fatty acids, 22:6/20:5 of the corals when compared to those of the suspended particulate organic matter. There is enrichment in L. pertusa of mono-unsaturated fatty acids and of δ 15 N relative to M. oculata. It is unclear whether this reflects different feeding strategies or assimilation/storage efficiencies of zooplankton tissue or different metabolism in the two coral species.

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“…In particular, for sterols and steroidal ketones of dinoflagellates, comparison was made with GCeMS data reported by Harvey et al (1988), Mansour et al (1999), Chapman (2002, 2004). The identification was confirmed by the analysis of TMS-ethers of pure standard for cholesterol, b-sitosterol and stigmasterol (Aldrich), and for dinosterol from the analysis of sterols extracted from the dinoflagellate Prorocentrum micans isolated from the North Adriatic and cultured in our laboratories (unpublished data).…”

Section: Quantitationmentioning

confidence: 99%

“…In comparison to the above-discussed sterols, dinosterol is usually considered more taxonomically specific, being characteristic of dinoflagellates, although it can be a minor sterol (or even lacking) in some species (Robinson et al, 1984;Withers, 1987;Harvey et al, 1988;Volkman et al, 1993;Mansour et al, 1999;Leblond and Chapman, 2002). Dinosterol has been found as an abundant component in several cultured dinoflagellates collected in the Adriatic Sea such as Prorocentrum micans, Lingulodinium polyedrum, Gymnodinium sp.…”

Section: Sterolsmentioning

confidence: 99%

The distribution of sterols and organic-walled dinoflagellate cysts in surface sediments of the North-western Adriatic Sea (Italy)

Sangiorgi

Fabbri

Comandini

et al. 2005

Estuarine, Coastal and Shelf Science

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The distributions of sterols and organic-walled dinoflagellate cysts (dinocysts) in five NW Adriatic Sea surface sediment samples were investigated. Samples are representative of areas differently influenced by freshwater inputs, mainly coming from the Po River. All the investigated samples exhibit the same suite of principal sterols, with cholest-5-en-3b-ol (cholesterol), 4a,23,24-trimethyl-5a-cholest-22E-en-3b-ol (dinosterol), 24-ethylcholest-5-en-3b-ol (sitosterol) and 24-methylcholesta-5,22E-dien-3b-ol (brassicasterol or epibrassicasterol) displaying the highest concentrations and relative abundances. The distribution of sterols in the samples is not related to their distance from the coast and/or with the C/N ratios and suggests a prevalent input of marine, autochthonous organic matter in the surface sediments. In particular, the high abundance of dinosterol underlines the importance of dinoflagellate productivity in this area and its contribution to the organic matter in sediments. However, absolute and relative abundances of dinosterol do not follow the trend observed for dinocyst concentrations in the investigated samples, with the exception of Spiniferites spp. cysts and cysts produced by Gonyaulax species.

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Lipid composition of the marine dinoflagellate Scrippsiella trochoidea

Cited by 73 publications

References 37 publications

Fatty acid and sterol composition of two evolutionarily closely related dinoflagellate morphospecies from cold Scandinavian brackish and freshwaters

Fatty acid and sterol composition of two evolutionarily closely related dinoflagellate morphospecies from cold Scandinavian brackish and freshwaters

Lipids and nitrogen isotopes of two deep-water corals from the North-East Atlantic: initial results and implications for their nutrition

The distribution of sterols and organic-walled dinoflagellate cysts in surface sediments of the North-western Adriatic Sea (Italy)

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