lipid formation induced by thiols in human monocytic leukemia cells

Ferreri, Carla; Kratzsch, Susanne; Brede, O.; Marciniak, Bronisław; Chatgilialoglu, Chryssostomos

doi:10.1016/j.freeradbiomed.2004.12.026

Cited by 80 publications

(103 citation statements)

References 37 publications

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“…This was confirmed by comparison with commercially available references and with a library of geometrical trans monounsaturated fatty acids (MUFA) and PUFA obtained by thiyl radical-catalyzed reaction of naturally occurring lipids. This technique has been used by others [13].…”

Section: Lipid Isolation From Erythrocyte Membranes and Gc Analysismentioning

confidence: 99%

Morbid Obesity is Associated to Altered Fatty Acid Profile of Erythrocyte Membranes

Genio¹

2015

J Diabetes Metab

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Background: Morbid obesity is a pathologic condition associated to an increased incidence of several chronic conditions associated to increased risk of mortality. Erythrocyte fatty acid composition has been candidate as a biomarker of oxidative stress and consequently on the rate of aging and ultimately lifespan. To our knowledge, no study has investigated the total spectrum of lipid composition of erythrocyte membranes from obese individuals. The aim of this study was to compare the fatty acid composition of erythrocyte membranes derived from morbidly obese patients with healthy matched controls and long-living descendants.Methods: Gas chromatography was employed to determine fatty acid percentage of erythrocyte membranes from investigated groups.Results: Erythrocyte membranes from obese subjects had significantly lower percentage of monounsaturated fatty acids (16.0 ± 0.8 vs. 23.0 ± 4.5, p<0.01) and total trans fatty acid (0.2 ± 0.1 vs. 1.3 ± 1.2, p<0.001) than controls. Obese fatty acid profiles showed increased percent of total polyunsaturated fatty acid (36.5 ± 1.4 vs. 27.3 ± 10.9, p<0.001), polyunsaturated fatty acid n-3 (5.2 ± 0.9 vs. 2.6 ± 1.8, p <0.001) and n-6 (31.3 ± 1.7 vs. 25.0 ± 10.1, p<0.001). Interestingly, when obese fatty acid profiles are compared with long-living descendants, the former show opposite trends in the percentages of the main saturated and unsaturated residues of membrane phospholipids compared with nonagenarian offspring. Conclusion:This study shows that the erythrocyte membranes fatty acid composition of obese subjects differs from general population in a way opposite to the long-living descendants. If confirmed by further investigations, the fatty acid equilibration may become an additional target in the multidisciplinary strategy aimed to treat the obesity epidemic.

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Section: Lipid Isolation From Erythrocyte Membranes and Gc Analysismentioning

confidence: 99%

Morbid Obesity is Associated to Altered Fatty Acid Profile of Erythrocyte Membranes

Genio¹

2015

J Diabetes Metab

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“…5,6 Recently, a structural change induced in cellular lipid metabolites by their conversion from the naturally occurring cis fatty acid geometry to the more stable trans configuration has been evidenced. 7 The trans lipid incorporation is known to affect membrane arrangement as well as lipid metabolism, and it is also linked to nutritional concern about chemically manipulated fats and oils. 8 Exposure of proteins to free radicals leads to modifications of the side chains of amino acid residues, in particular aromatic and sulfur-containing residues, to fragmentation and formation of protein aggregates via protein-protein crosslinking reactions.…”

Section: Introductionmentioning

confidence: 99%

Investigation of radical‐based damage of RNase A in aqueous solution and lipid vesicles

Torreggiani¹,

Tamba²,

Manco

et al. 2005

Biopolymers

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The gamma-irradiation of bovine pancreatic ribonuclease A (RNase A) in aqueous solution were investigated at different doses by vibrational spectroscopy as well as enzymatic assay, electrophoresis, and HPLC analysis. Both functional and structural changes of the protein were caused by attack of H(*) atoms and (*)OH radicals. In particular, Raman spectroscopy was shown to be a useful tool in identifying conformational changes of the protein structure and amino acidic residues that are preferential sites of the radical attack (i.e., tyrosine and methionine). After partial structural changes by the initial radical attack, the internal sulfur-containing amino acid residues were rendered susceptible to transformation. By using the biomimetic model of dioleoyl phosphatidyl choline vesicle suspensions containing RNase A, the damage to methione residues could be connected to a parallel alteration of membrane unsaturated lipids. In fact, thiyl radical species formed from protein degradation can diffuse into the lipid bilayer and cause isomerization of the naturally occurring cis double bonds. As a consequence, trans unsaturated fatty acids are formed in vesicles and can be considered to be markers of this protein damage.

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“…The samples were processed by membrane pellet isolation, trituration with 2:1 chloroform:methanol (v/v), 2-phase extraction, derivation to fatty acid methyl ester (FAME), and analyses using gas chromatography as described in Ferreri et al [17]. For both studies, the main fatty acids present in the trout liver were also compared with literature data.…”

Section: Membrane Fatty Acid Composition Analysismentioning

confidence: 99%

Cellular Responses to Tritium Exposure in Rainbow Trout: Hto- And Obt-Spiked Feed Exposure Experiments

et al. 2016

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Biological effects were evaluated in rainbow trout (Oncorhynchus mykiss) exposed to tritiated water (HTO) or food spiked with organically bound tritium (OBT). An HTO exposure study was conducted using a tritium activity concentration of 7000 Bq/L, and an OBT exposure study was conducted using a tritium activity concentration of 30 000 Bq/L. Following 140 days of in vivo HTO exposure, liver, heart, spleen, kidney, and brain cells did not show statistically significant differences in viability; kidney, liver, and spleen cells did not show significant differences in DNA double-strand break repair activity compared with control cells. Membrane fatty acid composition analysis was conducted on liver cells and no effects of HTO exposure could be detected. Following 140 days of in vivo OBT exposure, viability and DNA double-strand break repair activity were not statistically different from controls in liver, heart, spleen, kidney, and brain cells. Changes, however, were noted in the fatty acid composition of liver and muscle tissues. For both studies, all measurements were performed on each tissue and on a fraction of the same tissue that was exposed to a gamma 4 Gy dose in vitro to test for adaptive responses, and no effects were observed except for fatty acid composition. The findings demonstrated that membrane fatty acid composition is a sensitive marker and that microscopic evaluation of gamma-H2AX foci is more sensitive than the flow cytometric approach. These studies are the first to correlate uptake and depuration with biological health indicators in edible fish for tritium exposures within worldwide drinking water guidelines.

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lipid formation induced by thiols in human monocytic leukemia cells

Cited by 80 publications

References 37 publications

Morbid Obesity is Associated to Altered Fatty Acid Profile of Erythrocyte Membranes

Morbid Obesity is Associated to Altered Fatty Acid Profile of Erythrocyte Membranes

Investigation of radical‐based damage of RNase A in aqueous solution and lipid vesicles

Cellular Responses to Tritium Exposure in Rainbow Trout: Hto- And Obt-Spiked Feed Exposure Experiments

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