Lipid hydroperoxides in plants

Griffiths, Gareth; Leverentz, Michael K.; Silkowski, Helena; Gill, Narinder; Sánchez‐Serrano, José J.

doi:10.1042/bst0280837

Cited by 7 publications

(3 citation statements)

References 3 publications

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“…The principle of the FOX method is based on the oxidation of ferrous ions to ferric by the hydroperoxide activity in the acidic environment [90][91][92][93][94]. The exact mechanism of the sequence of radical reactions is not known, but the mechanism has been designed by Gupta et al [95] and is shown in reactions 1-4 (equation 2) [96].…”

Section: Ferrous Oxidation In Xylenol Orangementioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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Section: Ferrous Oxidation In Xylenol Orangementioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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“…It has the potential to be a rapid and inexpensive method for detecting incipient lipid peroxidation in plant tissue. To our knowledge, the FOX assay has been reported by only one other group for LOOH measurement in plant tissue (31,57), although it has been utilized for LOOH detection in edible vegetable oils (32,33). The main objective of this study was to adapt the FOX assay to more accurately detect the presence of LOOHs in plant tissue.…”

Section: Introductionmentioning

confidence: 99%

Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

DeLong

Prange

Hodges

et al. 2001

J. Agric. Food Chem.

152

118

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=7ee3580f-c134-4185-a6d3-cb87c5e10071 http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=7ee3580f-c134-4185-a6d3-cb87c5e10071 The ferrous oxidation-xylenol orange (FOX) assay was adapted for quantifying lipid hydroperoxides (LOOHs) in plant extracts. Excised pieces of several fruit and vegetable species were exposed to 83 kJ m -2 day -1 of biologically effective ultraviolet B irradiance (UV-B BE ) for 10-12 days to induce cellular oxidation. The LOOH and thiobarbituric acid reactive substance (TBARS) concentrations of these plant tissues were assessed with the FOX and iodometric assays for the former and a modified TBARS assay for the latter. There was generally good agreement between the FOX and iodometric methods both prior to and following the UV exposure. However, the iodometric assay appeared to have some difficulty in consistently quantifying lower LOOH levels (<11 µM), whereas the FOX assay measured LOOH concentrations as low as 5 µM. All tissues exhibited UV-induced increases in TBARS, indicating a marked degree of cellular oxidation in the exposed tissue segments. Compared with the iodometric assay, the FOX method consistently generated less variable LOOH values. The presence of authentic linoleic acid-OOHs in spiked avocado and spinach samples (11 µM) was identified with liquid chromatography-mass spectrometry techniques, which validated corresponding FOX assay results. The FOX method is inexpensive, is not sensitive to ambient O 2 or light levels, and can rapidly generate LOOH measurements. The physiological value of the FOX assay resides in its ability to measure initial rather than more advanced fatty acid oxidation; hence, early membrane-associated stress events in plant tissue can be detected.

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“…One to two percent of all membrane phospholipids constantly seem to be present in their peroxidized form. [104] What is the Evidence in Support of the Lipid Scenario?…”

Section: The Concept Of Lipid Noisementioning

confidence: 97%

Review

Sarán¹

2003

Free Radical Research

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Production of superoxide anion O2*- by the membrane-bound enzyme NADPH oxidase of phagocytes is a long-known phenomenon; it is generally assumed that O2*-helps phagocytes kill bacterial intruders. The details and the chemistry of the killing process have, however, remained a mystery. Isoforms of NADPH oxidase exist in membranes of nearly every cell, suggesting that reactive oxygen species (ROS) participate in intra- and intercellular signaling processes. What the nature of the signal is exactly, how it is transmitted, and what structural characteristics a receptor of a "radical message" must have, have not been addressed convincingly. This review discusses how the action of messengers is in agreement with radical-specific behavior. In search for the smallest common denominator of cellular free radical activity we hypothesize that O2*- and its conjugate acid, HO2*, may have evolved under primordial conditions as regulators of membrane mechanics and that isoprostanes, widely used markers of "oxidative stress", may be an adventitious correlate of this biologic activity of O2*-/HO2*. An overall picture is presented that suggests that O2*-/HO2* radicals, by modifying cell membranes, help other agents gain access to the hydrophobic region of phospholipid bilayers and hence contribute to lipid-dependent signaling cascades. With this, O2*-/HO2* are proposed as indispensable adjuvants for the generation of cellular signals, for membrane transport, channel gating and hence, in a global sense, for cell viability and growth. We also suggest that many of the allegedly O2*- dependent bacterial pathologies and carcinogenic derailments are due to membrane-modifying activity rather than other chemical reactions of O2*-/HO2*. A consequence of this picture is the potential evolution of the "radical theory of ageing" to a "lipid theory of aging".

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Lipid hydroperoxides in plants

Cited by 7 publications

References 3 publications

Automation of Methods for Determination of Lipid Peroxidation

Automation of Methods for Determination of Lipid Peroxidation

Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

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