Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

Abstract

Abstract. In this study, electrospun polycaprolactone (PCL) fibers are plasma-treated and chemically conjugated with cholesteryl succinyl silane (CSS). In addition to Raman spectroscopy, an immobilization study of DiO as a fluorescent probe of lipid membranes provides evidence supporting the CSS coating of plasma-treated PCL fibers. Further, anti-CD20 antibodies are used as a model protein to evaluate the potential of lipid-mediated protein immobilization as a mechanism to functionalize the CSS-PCL fiber scaffolds. Upon anti-CD20 functionalization, the CSS-PCL fiber scaffolds capture Granta-22 cells 2.4 times more than the PCL control does, although the two fiber scaffolds immobilize a comparable amount of anti-CD20. Taken together, results from the present study demonstrate that the CSS coating and CSS-mediated antibody immobilization offers an appealing strategy to functionalize electrospun synthetic polymer fibers and confer cell-specific functions on the fiber scaffolds, which can be mechanically robust but often lack biological functions.

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

“…Embedding bioactive molecules inside the nanofibers can cause the masking of their bioactivity [104]. The harsh conditions of the electrospinning might modify the bioactive sequences of the some peptide-based functionalities and proteins [108]. Moreover, because of the limited solubility of natural polymers in water, highly acidic and toxic solvents such as trifluoroacetic acid and 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) are being used for electrospinning.…”

“…After seeding Granta-22 B lymphoma cells, anti-CD20 functionalized fibers were shown to capture the cells 2.4 times more than the untreated fibers. Important TE applications may thus be improved by this lipid-mediated protein functionalization of nanofibers [108]. …”

Microparticles for Suspension Culture of Mammalian Cells

“…Synthetic materials are mechanically stronger, easier to fabricate, and modifiable to a specific function. Common synthetic, biodegradable materials, which are FDA approved for use in vivo , consist of poly­(lactic- co -glycolic acid) (PLGA), poly­(lactic acid) (PLA), , polycaprolactone (PCL), and polyethylene glycol (PEG) . PLGA has been widely used for cell anchorage, proliferation, and differentiation. , However, bare PLGA materials typically suffer from poor cell attachment unless the surface is chemically modified.…”