Lipid multilayer microarrays for in vitro liposomal drug delivery and screening

Kusi-Appiah, Aubrey; Vafai, Nicholas; Cranfill, Paula J.; Davidson, Michael W.; Lenhert, Steven

doi:10.1016/j.biomaterials.2012.02.023

Cited by 34 publications

(38 citation statements)

References 36 publications

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“…Among the emergent nanoscale drug delivery systems, lipids (liposomes) and polymers (polymeric nanoparticles and micelles) have demonstrated great potential clinical impact for the foreseeable future [2e5]. The lipids and micelles based nanoparticles have exhibited many advantages as drug delivery systems [6,7]. However, there are still many limitations to address, such as instability in circulation and inadequate tissue distribution.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable and amphiphilic block copolymer–doxorubicin conjugate as polymeric nanoscale drug delivery vehicle for breast cancer therapy

et al. 2013

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Section: Introductionmentioning

confidence: 99%

Biodegradable and amphiphilic block copolymer–doxorubicin conjugate as polymeric nanoscale drug delivery vehicle for breast cancer therapy

et al. 2013

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“…Later applications of cell microarrays were extended to screenings of small drug like molecules. In this case drugs have to be embedded in polymer or lipids to prevent rapid diffusion of molecules into the media and ensure direct delivery of drugs into the cell cluster 35,36 . The main limitation of cell microarray technology is the absence of barriers between spots and the culturing of cells in one medium, which leads to risk of crosscontamination and paracrine interaction between cell clusters and restricts this technology to cells of adherent nature.…”

Section: Introductionmentioning

confidence: 99%

Droplet-microarray on superhydrophobic–superhydrophilic patterns for high-throughput live cell screenings

et al. 2016

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Cell-based high content phenotypic screenings are widely used in fundamental research, pharmaceutical industry, and healthcare to simultaneously evaluate the effects of multiple compounds or gene over-expressions/knockdown on the phenotype of cells. Most screenings, especially in the industrial sector, are performed using microplate technology, which relies on high reagents and cell consumption as well as on expensive liquid handling robotics. Developing miniaturized screening platforms has been an important topic in the past decade. In this study we demonstrate the applicability of the Droplet-Microarray platform based on superhydrophobic-superhydrophilic patterning for cell-based high throughput screenings. We show the homogeneous seeding of cells and culturing of different adherent cell lines in individual droplets of different sizes. We demonstrate pipetting-free medium exchange enabling cell cultures in miniaturized droplet arrays for up to 5 days. We establish the method of reverse transfection and reverse drug screenings in individual nanoliter-size droplets by printing the transfection mixtures or drug molecules directly onto superhydrophilic spots prior to cell seeding.

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“…This fraction-dependent effect appears to be related to the specific interaction with liposomes that can affect their encapsulation efficiency and hence the efficiency of internalization should they be used in the hydrophobic form. This work demonstrates the value of further purification and screening of synthesized nanoparticles and opens the possibility of screening lipophilic nanomaterial drugs and libraries626364.…”

Section: Resultsmentioning

confidence: 80%

Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles

Kusi-Appiah

Mastronardi

Qian

et al. 2017

Sci Rep

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Specific size, shape and surface chemistry influence the biological activity of nanoparticles. In the case of lipophilic nanoparticles, which are widely used in consumer products, there is evidence that particle size and formulation influences skin permeability and that lipophilic particles smaller than 6 nm can embed in lipid bilayers. Since most nanoparticle synthetic procedures result in mixtures of different particles, post-synthetic purification promises to provide insights into nanostructure-function relationships. Here we used size-selective precipitation to separate lipophilic allyl-benzyl-capped silicon nanoparticles into monodisperse fractions within the range of 1 nm to 5 nm. We measured liposomal encapsulation and cellular uptake of the monodisperse particles and found them to have generally low cytotoxicities in Hela cells. However, specific fractions showed reproducibly higher cytotoxicity than other fractions as well as the unseparated ensemble. Measurements indicate that the cytotoxicity mechanism involves oxidative stress and the differential cytotoxicity is due to enhanced cellular uptake by specific fractions. The results indicate that specific particles, with enhanced suitability for incorporation into lipophilic regions of liposomes and subsequent in vitro delivery to cells, are enriched in certain fractions.

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Lipid multilayer microarrays for in vitro liposomal drug delivery and screening

Cited by 34 publications

References 36 publications

Biodegradable and amphiphilic block copolymer–doxorubicin conjugate as polymeric nanoscale drug delivery vehicle for breast cancer therapy

Biodegradable and amphiphilic block copolymer–doxorubicin conjugate as polymeric nanoscale drug delivery vehicle for breast cancer therapy

Droplet-microarray on superhydrophobic–superhydrophilic patterns for high-throughput live cell screenings

Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles

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