Lipid nanoparticle-encapsulated VEGFa siRNA facilitates cartilage formation by suppressing angiogenesis

Chen, Yi; Chen, Wei; Ren, Yiming; Li, Shuling; Liu, Miao; Xing, Jiahua; Han, Yang; Han, Yan; Tao, Ran; Guo, Lingli; Xiang, Sheng; Guo, Quanyi; Liu, Shuyun; Han, Yaling

doi:10.1016/j.ijbiomac.2022.09.065

Cited by 18 publications

(7 citation statements)

References 50 publications

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“…In addition, LNP-assisted mRNA vaccines have also been shown to activate CD8+ T cells and enhance immune cell recognition and attacks on tumors, playing an important role in tumor inhibition. Some studies [ 166 , 167 , 168 , 169 , 170 ] have pointed out that LNP carriers can help improve the stability and intracellular uptake efficiency of mRNA vaccines, thus enhancing the biological activity and persistence of mRNA vaccines. These findings provide strong support and evidence for the application of LNP-assisted mRNA vaccines in tumor therapy [ 171 ].…”

Section: Application Of Lnp-assisted Mrna Vaccines In Tumor Immunothe...mentioning

confidence: 99%

Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity

Wu,

Li,

Qian

et al. 2024

Vaccines

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In recent years, lipid nanoparticles (LNPs) have attracted extensive attention in tumor immunotherapy. Targeting immune cells in cancer therapy has become a strategy of great research interest. mRNA vaccines are a potential choice for tumor immunotherapy, due to their ability to directly encode antigen proteins and stimulate a strong immune response. However, the mode of delivery and lack of stability of mRNA are key issues limiting its application. LNPs are an excellent mRNA delivery carrier, and their structural stability and biocompatibility make them an effective means for delivering mRNA to specific targets. This study summarizes the research progress in LNP delivery carrier-assisted targeted controlled release mRNA vaccines in tumor immunity. The role of LNPs in improving mRNA stability, immunogenicity, and targeting is discussed. This review aims to systematically summarize the latest research progress in LNP delivery carrier-assisted targeted controlled release mRNA vaccines in tumor immunity to provide new ideas and strategies for tumor immunotherapy, as well as to provide more effective treatment plans for patients.

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Section: Application Of Lnp-assisted Mrna Vaccines In Tumor Immunothe...mentioning

confidence: 99%

Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity

Wu,

Li,

Qian

et al. 2024

Vaccines

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“…[361] Similarly, Chen et al reported a cartilage scaffold, a gelatin methacryloyl (GelMA)-based hydrogel, combined with siRNA-loaded LNP, chondrocytes, and adiposederived stem cells for suppressing microvascular infiltration and generating new cartilage in mice (Figure 15b). [362] Another example of bioengineering applications includes the work by Yalgın et al which reported the use of LNP in the determination of mouse embryogenic NIH-3T3 fibroblast growth on polylactic acid (PLA)-based 3D printed human auricular models. [382] LNP containing cyclosporine A (CycA) and coenzyme Q10 (Q10) were used to evaluate whether cell proliferation on scaffolds could be induced.…”

Section: Bioengineering Applications and Basic Science Studymentioning

confidence: 99%

mentioning

confidence: 99%

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

Seo

Jeon

Kwon

et al. 2023

Adv Healthcare Materials

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The recent development of RNA-based therapeutics in delivering nucleic acids for gene editing and regulating protein translation has led to the effective treatment of various diseases including cancer, inflammatory and genetic disorder, as well as infectious diseases. Among these, lipid nanoparticles (LNP) have emerged as a promising platform for RNA delivery and have shed light by resolving the inherent instability issues of naked RNA and thereby enhancing the therapeutic potency. These LNP consisting of ionizable lipid, helper lipid, cholesterol, and poly(ethylene glycol)-anchored lipid can stably enclose RNA and help them release into the cells' cytosol. Herein, the significant progress made in LNP research starting from the LNP constituents, formulation, and their diverse applications is summarized first. Moreover, the microfluidic methodologies which allow precise assembly of these newly developed constituents to achieve LNP with controllable composition and size, high encapsulation efficiency as well as scalable production are highlighted. Furthermore, a short discussion on current challenges as well as an outlook will be given on emerging approaches to resolving these issues.

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“…siRNA-LNP scaffolds enable the loading of both seeded cells and VEGF siRNA-LNPs in GELMA hydrogel resulting in promoted cartilage formation. [180,183,185] RNA loaded exosome Exosomal miRNAs regulate osteoblast differentiation. MSC exosomes may affect the Wnt pathway and PI3K/Akt pathway.…”

Section: Rna Loaded Scaffoldmentioning

confidence: 99%

“…For example, MA/Cells+VEGFa siRNA‐LNP scaffolds were constructed using microfluidic technology to enable the loading of both seeded cells and VEGF siRNA‐LNPs in GELMA hydrogels. Due to the upregulation of HIF‐1α and FOXO transcription factors, the hypoxic and nutritional environment promoted cartilage formation resulting in significant upregulation of SOX9, COL‐II, and ACAN, which contributed to the superior regeneration of cartilage using this strategy [ 185 ] (Figure 9B). These findings demonstrate the potential of siRNA‐based strategies for cartilage reconstruction.…”

Section: Nucleic Acid Nanotechnology In Bone and Cartilage Regenerationmentioning

confidence: 99%

Harnessing Nucleic Acids Nanotechnology for Bone/Cartilage Regeneration

Han

Cao²,

et al. 2023

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The effective regeneration of weight‐bearing bone defects and critical‐sized cartilage defects remains a significant clinical challenge. Traditional treatments such as autologous and allograft bone grafting have not been successful in achieving the desired outcomes, necessitating the need for innovative therapeutic approaches. Nucleic acids have attracted significant attention due to their ability to be designed to form discrete structures and programmed to perform specific functions at the nanoscale. The advantages of nucleic acid nanotechnology offer numerous opportunities for in‐cell and in vivo applications, and hold great promise for advancing the field of biomaterials. In this review, the current abilities of nucleic acid nanotechnology to be applied in bone and cartilage regeneration are summarized and insights into the challenges and future directions for the development of this technology are provided.

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Lipid nanoparticle-encapsulated VEGFa siRNA facilitates cartilage formation by suppressing angiogenesis

Cited by 18 publications

References 50 publications

Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity

Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity

High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications

Harnessing Nucleic Acids Nanotechnology for Bone/Cartilage Regeneration

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