Lipid Nanoparticles for Gene Delivery

Zhao, Yi; Huang, Leaf

doi:10.1016/b978-0-12-800148-6.00002-x

Cited by 146 publications

(89 citation statements)

References 91 publications

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“…The latter provide several advantages including higher stability, low toxicity, and greater efficiency. [11][12][13][14] In addition, systems based on LNPs can be easily modified with other ligands and devices for controlled biodistribution and internalization into specific cells.…”

Section: Introductionmentioning

confidence: 99%

Lipid Nanoparticles for Cell-Specific <i>in Vivo</i> Targeted Delivery of Nucleic Acids

Khalil

Younis

Kimura

et al. 2020

Biological & Pharmaceutical Bulletin

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The last few years have witnessed a great advance in the development of nonviral systems for in vivo targeted delivery of nucleic acids. Lipid nanoparticles (LNPs) are the most promising carriers for producing clinically approved products in the future. Compared with other systems used for nonviral gene delivery, LNPs provide several advantages including higher stability, low toxicity, and greater efficiency. Additionally, systems based on LNPs can be modified with ligands and devices for controlled biodistribution and internalization into specific cells. Efforts are ongoing to improve the efficiency of lipid-based gene vectors. These efforts depend on the appropriate design of nanocarriers as well as the development of new lipids with improved gene delivery ability. Several ionizable lipids have recently been developed and have shown dramatically improved efficiency. However, enhancing the ability of nanocarriers to target specific cells in the body remains the most difficult challenge. Systemically administered LNPs can access organs in which the capillaries are characterized by the presence of fenestrations, such as the liver and spleen. The liver has received the most attention to date, although targeted delivery to the spleen has recently emerged as a promising tool for modulating the immune system. In this review, we discuss recent advances in the use of LNPs for cellspecific targeted delivery of nucleic acids. We focus mainly on targeting liver hepatocytes and spleen immune cells as excellent targets for gene therapy. We also discuss the potential of endothelial cells as an alternate approach for targeting organs with a continuous endothelium.

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Section: Introductionmentioning

confidence: 99%

Lipid Nanoparticles for Cell-Specific <i>in Vivo</i> Targeted Delivery of Nucleic Acids

Khalil

Younis

Kimura

et al. 2020

Biological & Pharmaceutical Bulletin

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“…Spontaneous electrostatic interactions between these non-viral carriers and nucleic acids lead to the formation of lipoplexes and polyplexes, respectively, which offer useful properties for gene delivery [14]. Cationic lipids offer efficient binding to negatively charged cell surface constituents to stimulate uptake, and cationic lipids can further interact with the endosomal membrane to facilitate cytoplasmic delivery [15]. Alternatively, cationic polymers, including branched, linear, and dendritic structures, offer flexible polymer chemistries that facilitate design and formulation of nanoscale polyplexes with tailorable properties, with facile polymer modification for use in plethora of biomedical applications [16].…”

Section: Introductionmentioning

confidence: 99%

Gene delivery by peptide-assisted transport

Thapa

Sullivan

2018

Current Opinion in Biomedical Engineering

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The diverse amino acid chemistries and secondary structures in peptides provide ‘minimalist’ mimics of motifs in proteins and offer many ideal properties for targeted delivery approaches. Several non-viral vectors (polymers and lipids) have been studied for their potential applications in gene delivery. However, non-specific uptake, lack of targeting, inability to escape endosomes, and inefficient nuclear delivery limit their application. Peptide-assisted trafficking of non-viral vectors can potentially overcome these biological barriers to improve gene delivery through targeted uptake using key cell-surface receptors (e.g., integrins, growth factor receptors, and G-protein coupled receptors); membrane disruption for endosomal escape; and nuclear importation. Furthermore, the capacity of peptides to regulate spatio-temporal control over gene delivery opens multi-faceted avenues for effective gene delivery in a variety of complex applications. Rigorous on-going in vitro and in vivo studies utilizing peptides for targeted and microenvironment-sensitive gene delivery could promote their widespread clinical usage.

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“…There are a number of nanocarriers used for gene delivery ( Figure 25 ) [ 489 ] applications which are based on lipids [ 490 , 491 ], liposomes [ 492 , 493 , 494 ], dendrimers [ 495 ], polymers [ 496 , 497 ], graphene [ 498 , 499 ], carbon nanotubes (CNTs) [ 500 , 501 ], mesoporous silica [ 502 ], gold nanoparticles [ 503 , 504 ], magnetic nanoparticles [ 505 , 506 ], and other types of inorganic nanoparticles.…”

Section: Applications Of Nanobiomaterialsmentioning

confidence: 99%

Comprehensive Survey on Nanobiomaterials for Bone Tissue Engineering Applications

et al. 2020

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One of the most important ideas ever produced by the application of materials science to the medical field is the notion of biomaterials. The nanostructured biomaterials play a crucial role in the development of new treatment strategies including not only the replacement of tissues and organs, but also repair and regeneration. They are designed to interact with damaged or injured tissues to induce regeneration, or as a forest for the production of laboratory tissues, so they must be micro-environmentally sensitive. The existing materials have many limitations, including impaired cell attachment, proliferation, and toxicity. Nanotechnology may open new avenues to bone tissue engineering by forming new assemblies similar in size and shape to the existing hierarchical bone structure. Organic and inorganic nanobiomaterials are increasingly used for bone tissue engineering applications because they may allow to overcome some of the current restrictions entailed by bone regeneration methods. This review covers the applications of different organic and inorganic nanobiomaterials in the field of hard tissue engineering.

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Lipid Nanoparticles for Gene Delivery

Cited by 146 publications

References 91 publications

Lipid Nanoparticles for Cell-Specific <i>in Vivo</i> Targeted Delivery of Nucleic Acids

Lipid Nanoparticles for Cell-Specific <i>in Vivo</i> Targeted Delivery of Nucleic Acids

Gene delivery by peptide-assisted transport

Comprehensive Survey on Nanobiomaterials for Bone Tissue Engineering Applications

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