Lipid nutrition and metabolism of cultured mammalian cells☆

Spector, Arthur A.; Mathur, Satya N.; Kaduce, Terry L.; Hyman, Bradley T.

doi:10.1016/0163-7827(80)90003-x

Cited by 213 publications

(84 citation statements)

References 109 publications

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“…A similar situation has been described in tissue culture cells which tend to form lipid inclusion bodies, containing triacylglycerols, when they are growing in lipid rich culture media containing either flee fatty acid or plasma protein (35,34). Moreover, many types of cells modify the composition of their membrane phospholipid fatty acids during short-term culture if the type of lipid in the culture medium is restricted or enriched with a particular fatty acid (3,33). The presence or reducing the number of these droplets doesn't affect subsequent in vivo viability of fresh embryos after transfer.…”

Section: Discussionmentioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

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To study the effect of partial removal of intracytoplasmatic lipids from bovine zygotes on their in vitro and in vivo survival, presumptive zygotes were delipidated by micromanipulation and cocultured with Veto cells in B2+10% FCS. Blastocyst rates of delipidated (n=960), sham (centrifuged but not delipidated, n=830) and control embryos (n=950) were 42.1, 42.3 and 39.9% respectively (P > 0.05). Day 7 blastocysts derived from delipidated zygotes had a mean of 123.9 + 45.6 nuclei compared to 137.5 + 32.9 for control blastocysts (P > 0.05). The full-term development of delipidated blastocysts after single transfer to recipients was similar to that of control IVF blastocysts (41.2% vs 45.4% respectively). To assess the effect of delipidation on the embryo tolerance to freczing/thawing, delipidated (n=73), control (n=67) and shmn (n=50) Day 7 blastocysts were frozen in 1.36 M glycerol + 0.25 M sucrose in PBS. After thawing, embryos were cocultured for 72 h with Vero cells in B2+10% FCS. Survival rates at 24 h were not significantly different between groups. However, in the delipidated group, the survival rate after 48 h in culture was significantly higher than in the control group (56.2 vs 39.8, P < 0.02), resulting in a higher hatching rate after 3 days in culture (45.2 vs 22.4, P < 0.02). Pregnancy rates for delipidated and control frozen/thawed embryos were respectively 10.5 and 22.2% (P > 0.05). Electron microscopic observations showed much fewer lipid droplets (and smaller) in delipated blastocysts than in controls. Taken together, our data show that delipidation of one cell stage bovine embryos is compatible with their normal development to term and has a beneficial effect on their tolerance to freezing and thawing at the blastocyst stage. This procedure, however, alters the developmental potential of such blastocysts, suggesting that maternally inherited lipid stores interfere with metabolic recovery after thawing.

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Section: Discussionmentioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

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“…Hepatic ACAT activity and cholesteryl ester levels are sensitive to the fatty acid composition of dietary lipid, being higher in animals fed unsaturated fatty acids compared to those fed saturated fatty acids (29,30). We previously showed in the hamster that hepatic LDL receptor …”

Section: Resultsmentioning

confidence: 99%

“…In hepG2 cells it has been shown that ACAT inhibitors accelerate the intracellular degradation of apo B (49). We speculate that overexpression of hACAT-1 resulted in decreased intracellular degradation of apo B. MTP is required for the assembly of apo B containing lipoproteins (4,5,50,51) and is rate-limiting in hepatoma cells and primary hepatocytes (52) Hepatic ACAT activity (30) and cholesteryl ester concentrations (29) are higher in animals fed unsaturated fatty acids than in animals fed saturated fatty acids. Conversely, plasma total and LDL cholesterol concentrations are reduced in animals fed unsaturated fatty acids compared to animals fed saturated fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the observation that hepatic ACAT activity is higher in animals fed unsaturated fatty acids than in those fed saturated fatty acids (30), it has been hypothesized that unsaturated fatty acids, by enhancing ACAT activity, may reduce the amount of free cholesterol in a putative regulatory pool that feeds back on LDL receptor expression (27)(28)(29)31,32).…”

Section: Introductionmentioning

confidence: 99%

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Role of Acyl-Coenzyme A:Cholesterol Acyltransferase-1 in the Control of Hepatic VLDL Secretion and LDL Receptor Expression in the Mouse and Hamster

Spady

Willard

Meidell

2000

Journal of Biological Chemistry

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“…Most cultured mammalian cells are found to be able to synthesize lipids from different nutrients contained in the medium, but when an appropriate supply of lipids is present in the culture fluid, biosynthesis of lipids is inhibited (Spector et al 1981;Delplanque & Jacotot 1987;Fritz & Fajas 2010). It has been shown that cells in culture take up fatty acids from the extracellular medium which results in their fatty acid composition mirroring that of the medium where they are cultured (Spector & Yorek 1985).…”

Section: Introductionmentioning

confidence: 99%

Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells

García-Pelayo

Garcia-Peregrin

Martínez-Cayuela

2013

Frontiers in Life Science

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Cell membrane proteins are sensitive to their fatty acid environment; however, the effects of those fatty acids are not completely understood. In this work, we have optimized a model for the study of membrane protein regulation, which allows the investigation of lipid metabolism in cultured tumor cells. The membrane fatty acid composition of Reuber H35 hepatoma cells was modified by enriching phospholipids in individual fatty acids of different length and degree of saturation. Lauric, myristic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were supplemented to the culture medium of cells growing in presence or absence of lipoproteins. The four fatty acids were incorporated into membrane phospholipids in a dosedependent manner. Lauric and myristic acids were actively metabolized, increasing percentages of longer and unsaturated chain fatty acids, but they did not modify the unsaturated index. As expected, EPA and DHA significantly decreased the unsaturated index. Incorporation of EPA and DHA was accompanied of a significant decrease of total monounsaturated fatty acids and an increase of total saturated fatty acids. Our results demonstrate that Reuber H35 hepatoma cells constitute a suitable model to manipulate the lipid composition of the cell membranes and perform functionality studies on the membrane proteins in response to their lipid environment.

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Lipid nutrition and metabolism of cultured mammalian cells☆

Cited by 213 publications

References 109 publications

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Role of Acyl-Coenzyme A:Cholesterol Acyltransferase-1 in the Control of Hepatic VLDL Secretion and LDL Receptor Expression in the Mouse and Hamster

Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells

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