Lipid organization in erythrocyte membrane microvesicles

Scott, Stephen; Pendlebury, S A; Green, C

doi:10.1042/bj2240285

Cited by 24 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the relative area of the inner leaflet gets smaller, it should be seen as a relative loss of coneshaped PLs such as PE species with long and highly unsaturated acyl chains, e.g., PE 38:4. This membrane curvature effect could theoretically lead to some physicochemically-driven selection of lipids to accomplish energetically favorable packing of the membrane [40][41][42]. In addition, the shorter chains, even one, influence the efflux properties of the PL molecules.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage

Laurén

Tigistu-Sahle

Valkonen

et al. 2018

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

View full text Add to dashboard Cite

Red blood cells (RBCs) are stored up to 35-42days at 2-6°C in blood banks. During storage, the RBC membrane is challenged by energy depletion, decreasing pH, altered cation homeostasis, and oxidative stress, leading to several biochemical and morphological changes in RBCs and to shedding of extracellular vesicles (EVs) into the storage medium. These changes are collectively known as RBC storage lesions. EVs accumulate in stored RBC concentrates and are, thus, transfused into patients. The potency of EVs as bioactive effectors is largely acknowledged, and EVs in RBC concentrates are suspected to mediate some adverse effects of transfusion. Several studies have shown accumulation of lipid raft-associated proteins in RBC EVs during storage, whereas a comprehensive phospholipidomic study on RBCs and corresponding EVs during the clinical storage period is lacking. Our mass spectrometric and chromatographic study shows that RBCs maintain their major phospholipid (PL) content well during storage despite abundant vesiculation. The phospholipidomes were largely similar between RBCs and EVs. No accumulation of raft lipids in EVs was seen, suggesting that the primary mechanism of RBC vesiculation during storage might not be raft -based. Nonetheless, a slight tendency of EV PLs for shorter acyl chains was observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage

Laurén

Tigistu-Sahle

Valkonen

et al. 2018

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

View full text Add to dashboard Cite

show abstract

“…Circulating MP are most notably derived from platelets [65], but also from erythrocytes [66], blood leucocytes [26] and endothelial cells [23,63,64,67]. In recent years, MP are increasingly studied in various disease states, in which their numbers, source and composition are altered.…”

Section: Microparticles and Pathologiesmentioning

confidence: 99%

Cell vesiculation and immunopathology: implications in cerebral malaria

Coltel

Combes

Wassmer

et al. 2006

Microbes and Infection

View full text Add to dashboard Cite

“…In particular, the aminophospholipids, phosphatidyl-serine and -ethanolamine, are no longer sequestered to the inner leaflet of the membrane, and are instead homogenously distributed across the microvesicle bilayer membrane. [16,17] …”

Section: Introductionmentioning

confidence: 99%

Exosomes and Microvesicles: Identification and Targeting By Particle Size and Lipid Chemical Probes

2014

View full text Add to dashboard Cite

Exosomes and microvesicles are two classes of submicroscopic vesicle released by cells into the extracellular space. Collectively referred to as extracellular vesicles, these membrane containers facilitate important cell-cell communication by carrying a diverse array of signaling molecules, including nucleic acids, proteins, and lipids. Recently, the role of extracellular vesicle signaling in cancer progression has become a topic of significant interest. Methods to detect and target exosomes and microvesicles are needed to realize applications of extracellular vesicles as biomarkers and, perhaps, therapeutic targets. Detection of exosomes and microvesicles is a complex problem as they are both submicroscopic and of heterogeneous cellular origins. In this Minireview, we highlight the basic biology of extracellular vesicles, and address available biochemical and biophysical detection methods. Detectible characteristics described here include lipid and protein composition, and physical properties such as the vesicle membrane shape and diffusion coefficient. In particular, we propose that detection of exosome and microvesicle membrane curvature with lipid chemical probes that sense membrane shape is a distinctly promising method for identifying and targeting these vesicles.

show abstract

Lipid organization in erythrocyte membrane microvesicles

Cited by 24 publications

References 40 publications

Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage

Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage

Cell vesiculation and immunopathology: implications in cerebral malaria

Exosomes and Microvesicles: Identification and Targeting By Particle Size and Lipid Chemical Probes

Contact Info

Product

Resources

About