Lipid Peroxidation Activity and Immune Response in Modeling Inflammatory and Degenerative Damage to the Periodontium of Rats

Abstract

The aim of this study was to assess changes in the lipid peroxidation activity and immune response in modeling inflammatory and degenerative damage to the soft tissues of the periodontium of Wistar rats. Materials and Methods: The experiments were carried out on male Wistar rats weighing 220-250g. Modeling the inflammatory and degenerative damage to the soft tissues of the periodontium in animals (n=10) of the test group (TG) was carried out using a single dose of a 2% formaldehyde aqueous solution injected into each side of the outer part of the gums (0.15 ml) at the level of the lower molars of anesthetized animals. The control group (CG) of animals (n=10) received an injection of physiological saline solution in the same volume. In 3 days after damage modeling, the animals were killed by decapitation and blood samples were drawn for testing. The intensity of LPO processes in the blood was estimated by content of conjugated dienes and TBA-reactive substances (TBARS). The status of the AOD system was determined by the SOD activity and GSH level. The immune status of the animals was assessed by several indicators as leukocyte migration inhibition test (LMIT), the phagocytic reaction of neutrophils, and oxygen-dependent and oxygen-independent microbicidal activity of neutrophils. Results: The results obtained showed that the modeling of inflammatory and degenerative damage to the periodontium in rats was characterized by significant changes, affecting both the LPO system and the immune response. An LMIT conducted with various mitogens demonstrated the development of immunodeficiency in TG characterized by high activity of the inflammatory process. Immune disorders in animals of TG were also supported by changes in the phagocytosis system. Low levels on the integral NBT test indicate disorders in the state of oxygen-dependent microbicidal systems of phagocytes. The assessment of the activity of oxygen-independent microbicidal systems of phagocytes in LCT also found disorders in the immune status of animals of TG. Conclusion: Inflammatory and degenerative damage of the soft tissues of the periodontium in rats is accompanied by pronounced disorders in both the LPO system and the immune response.

Histological study of the effect of bacterial lysates on the state of periodontal tissue in experimental periodontitis in rats

“…Similar drugs ("Ribomunil", "Imudon" (France), etc.) provide fast nonspecific protection against viruses, bacteria, ribs and long-term specific protection against the main pathogens in IPD [5,6]. Such agents are a lyophilized lysate of certain groups of bacteria most commonly found in dental plaque.…”