Lipid Peroxidation and Antioxidant Enzymes in Rat Tissues in Pyrethroid Toxicity: Possible Involvement of Reactive Oxygen Species

Kale, Manisha; Rathore, Nisha; John, Susan; Bhatnagar, Deepak

doi:10.1080/13590849961825

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…It is well known that ROS may cause DNA strandbreaks [Zegura et al, 2004]. It was reported that cypermethrin induced oxidative stress and generation of oxygen species in experimental systems [Kale et al, 1999;Giray et al, 2001]. It is not clear whether a-cypermethrin itself, or one of its metabolites, is responsible for the genotoxicity of this compound.…”

Section: Discussionmentioning

confidence: 99%

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Kocaman

Topaktaş

2008

Environ and Mol Mutagen

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a-Cypermethrin, a highly active pyrethroid insecticide, is effective against a wide range of insects encountered in agriculture and animal husbandry. The potential genotoxicity of a commercial formulation of a-cypermethrin (Fastac 100 EC, containing 10% a-cypermethrin as the active ingredient) on human peripheral lymphocytes was examined in vitro by sister chromatid exchange (SCE), chromosomal aberrations (CAs), and micronucleus (MN) tests. The human lymphocytes were treated with 5, 10, 15, and 20 lg/ml of a-cypermethrin for 24-and 48-hr. a-Cypermethrin induced SCEs and CAs significantly at all concentrations and treatment times and MN formation was significantly induced at 5 and 10 lg/ml of a-cypermethrin when compared with both the control and solvent control. Binuclear cells could not be detected sufficiently in the highest two concentration of a-cypermethrin (15 and 20 lg/ml) for both the 24-and 48-hr treatment times. a-Cypermethrin decreased the proliferation index (PI) at three high concentrations (10, 15, and 20 lg/ml) for both treatment periods as compared with the control groups. In addition, acypermethrin reduced both the mitotic index (MI) and nuclear division index (NDI) significantly at all concentrations for two treatment periods. The PI and MI were reduced by a-cypermethrin in a concentration-dependent manner during both treatment times. In general, a-cypermethrin showed higher cytotoxic and cytostatic effects than positive control (MMC) at the two highest concentrations for the 24-and 48-hr treatment periods. The present study is the first to report the genotoxic and cytotoxic effects of commercial formulation of a-cypermethrin in peripheral blood lymphocytes. Environ. Mol. Mutagen. 50:27-36, 2009. V V C 2008 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Kocaman

Topaktaş

2008

Environ and Mol Mutagen

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“…Treatment of RAW264.7 cells with AF and its enantiomers leads to different increases in intracellular ROS. A close relationship exists between generation of intracellular ROS and DNA damage [17]. In our experiments, DNA damage studies were carried out using a comet assay.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, evaluation of the level of ROS is another important biomarker for assessing cytotoxicity and genotoxicity. Previous studies have indicated that some pesticides were able to induce intracellular ROS generation [17]. At the same time, an increase in intercellular ROS often leads to DNA damage and genotoxicity [18].…”

Section: Introductionmentioning

confidence: 99%

Enantioselectivity in the immunotoxicity of the insecticide acetofenate in an in vitro model

Zhao

Liu

2009

Enviro Toxic and Chemistry

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Chiral pesticides with an asymmetrical center in their molecular structures possess enantioselectivity, not only in their pesticidal activities toward targeted organisms but also in toxicities to nontargeted organisms. Despite the fact that chiral pesticides deserve particular attention because of their ubiquitous presence in living and working environments, there has been limited research into their enantioselectivity in chronic toxicity. The immunotoxicity of chiral pesticides with respect to enantioselectivity has not been studied before. In this study, the role of enantioselectivity in the immunotoxicity of acetofenate (AF), an organochlorine insecticide, was investigated in an in vitro macrophage cell line model. Results of the cytotoxicity assay showed a clear dose-dependent growth inhibition effect of AF with enantioselectivity on RAW264.7 cells. S-(+)-AF was clearly more toxic to macrophages than R-(-)-AF and rac-AF. This work also demonstrated that S-(+)-AF possesses the strongest effects in induction of intracellular reactive oxygen species, DNA damage, and upregulation of p53 gene expression. These results, for the first time, show stark selectivity between enantiomers in their ability to induce macrophage-involved immunotoxicity of AF. These results suggest that assessment of the environmental safety and health risk of chiral contaminants should consider the role of enantioselectivity in immunotoxicity. In addition, our study will improve the knowledge of the role of enantioselectivity in immunotoxicity of chiral contaminants.

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“…Previous studies report the involvement of ROS in pyrethroid toxicity, in particular it increases lipid peroxidation and alters the antioxidant system in plasma membrane of heart cells [52,53].…”

Section: Discussionmentioning

confidence: 99%

Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

2010

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Pollutants including insecticides have been recently reported to be a risk factor involved in various diseases. Permethrin, a member of the family of synthetic pyrethroids, is widely used as insecticide in agriculture and other domestic applications. To investigate possible cardiotoxicity, we had examined different concentrations of permethrin on the freshly isolated rat heart cells using the alkaline comet assay. A significant difference in % tail DNA between all concentrations of permethrin (5, 10, 20 microM) and vehicle (control) without enzymes and with Fpg-treated cells were measured. The results indicated that permethrin induced oxidative damage to purine bases in the heart cells. Pyrimidines oxidation was evaluated using Endonuclease III (Endo III), but the results did not reveal any significant changes. After permethrin exposure, cells were studied to evaluate their DNA repair capacity. A complete DNA repair at 10 and 20 microM was measured after 30 and 60 min of repair intervals. Significant change in plasma membrane fluidity at different depths of bilayer was measured following permethrin treatment. Membrane fluidity in the hydrophilic-hydrophobic region was reduced, while the hydrophobic inner resulted more fluid following permethrin treatment of heart cells. This work points to standardize conditions applicable to ex vivo cells following in vivo treatment in order to study the cardiotoxicity of insecticide.

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Lipid Peroxidation and Antioxidant Enzymes in Rat Tissues in Pyrethroid Toxicity: Possible Involvement of Reactive Oxygen Species

Cited by 14 publications

References 12 publications

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Enantioselectivity in the immunotoxicity of the insecticide acetofenate in an in vitro model

Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

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