Lipid peroxidation and antioxidant status in blood of patients with uterine myoma, endometrial polypus, hyperplastic and malignant endometrium

Abstract

Oxidative stress is considered to be involved in pathogenesis of many disorders of the female genital tract. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of uterine diseases in order to evaluate the extent of oxidative stress in blood of such patients. Blood samples of healthy subjects and gynecological patients were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides. The results show that alterations of measured parameters vary with the enzyme type and diagnosis. However, both reduction in antioxidants and elevation of lipid peroxidation were observed in general. Lipid hydroperoxides level was negatively correlated to superoxide dismutase and glutathione peroxidase activities, as well as positively correlated to catalase activity. In addition, the lipid hydroperoxides/ glutathione peroxidase ratio was found to be increased, according to the type of uterine disease. The obtained results show that perturbation of antioxidant status is more pronounced in blood of patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions, compared to those with benign uterine changes such as polypus and myoma.

A STUDY OF SERUM MDA & TAC IN PATIENTS OF CERVICAL CANCER

“…These results were also in agreement with other investigations done by Pejic et al, 2006, which showed an increase in concentration of lipid peroxidation products, as markers of oxidative stress whereas the level of antioxidants decreased in uterine cancer patients. The rise in MDA concentration could be due to increased generation of ROS due to the excessive oxidative damage generated in uterine cancer patients, oxygen radical production, which increases with clinical progression of diseases, involves increased lipid peroxidation, as a result of which there are cellular membrane degeneration and DNA damages 6 (Naidu MSK, et al 2007).…”

“…The oxygen radical production increases with clinical progression of diseases and involves increased lipid peroxidation. As a result of this, there are cellular membrane degeneration and DNA damage 9 (Pejic, et al 2006). The rise in the levels of MDA indicate excessive lipid peroxidation as a consequence of the increase in free radical generation.…”

“…The rise in the levels of MDA indicate excessive lipid peroxidation as a consequence of the increase in free radical generation. These free radicals may cause marked alterations in the function of the cell membrane and also structural organisation of DNA, hence causing mutations, thus it can be stated that lipid peroxidation product is one of the possible causes of uterine cancer progression 9 (Pejic, et al 2006). The total antioxidant capacity (TAC) instead of measuring the antioxidant capacity of a single compound, measures the antioxidant capacity of whatever antioxidants in the biological sample.…”

Oxidative stress and prolidase activity in women with uterine fibroids

“…Decreased levels of Cat and prolidase enzyme activity in the serum of the fi broid group with respect to the control group were remarkable. Pejic et al (2006) had studied oxidant and antioxidant parameters in the serum of the patients with uterine myoma, endometrial polyps, hyperplastic and malignant endometrium and found elevated serum levels of LOOH in the uterine myoma patients compared to the control group. On the contrary, we could not fi nd a signifi cant diff erence in the serum LOOH levels between the study and control groups.…”

Genetic and Genomics of Uterine Myomas

“…Pejić and collaborators observed that perturbation of antioxidant status is more pronounced in blood of patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions, compared to those with benign uterine changes such as polypus and myoma [ 78 ]. Although information about the role of different signaling pathways highlight the role of proteins in the onset and development of myomas, comparative proteome profi ling studies are still lacking.…”