Lipid Peroxidation in Hepatopancreas, Gill, and Hemolymph of Male and Female Crabs Platyxanthus orbignyi after Air Exposure

Abstract

Levels of lipid peroxidation in hepatopancreas (HP), gill (G), and hemolymph (HYM) of stone violaceous crab Platyxanthus orbignyi (Milne Edwards and Lucas (1843)) were performed to examine the effect of short exposure to air. After four hours animals were collected, 14 from exposure to air and 10 from seawater were dissected and their lipid peroxidation (LPO) levels were evaluated using the ferrous oxidation-xylenol orange (FOX) method, in gill, hepatopancreas, and hemolymph. The total mortality of those crabs was evaluated after seven hours at 22 ± 1 °C on exposure to air conditions. Levels of LPO in hepatopancreas (female/male = 4.68 ± 1.60/5.12 ± 1.59 Eq-H2O2/g wet tissue) and hemolymph (female/male = 1.48 ± 1.42/1.28 ± 1.06 Eq-H2O2/g wet tissue) displayed no significant differences, in contrast, gills displayed significant differences (male/female = 5.63 ± 0.83/4.63 ± 0.44 Eq-H2O2/g wet tissue, p < 0.05). The results showed that air exposure in the short term in this study induces a different response in oxidative stress levels and damage could be accompanied by accumulation of peroxide lipids (LOOH). These results suggest that different organs can show different responses to oxidative stress between male and female crabs to this species.

Effect of Diet on Growth Performance of First Crab Stage Callinectes sapidus Rathbun, 1896 (Brachyura: Portunidae): A Comparison of Three Different Regimens

“…During the growth study, the animals were immersed in water all the time. Crabs were avoided in stress due to possible hypoxia from exposure to air [ 33 ], as this may interfere with their mortality. When removing the residues, no difference was observed between the food and the shells because the animal crushed them.…”

Mangrovimonas cancribranchiae sp. nov., a novel bacterial species associated with the gills of the fiddler crab Cranuca inversa (Brachyura, Ocypodidae) from Red Sea mangroves

“…S8). Remarkably, the presence of a complete carotenoid gene cluster and the synthesis of flexirubin pigment, recently acknowledged for its antioxidant properties [59,60], suggests a potential adaptive advantage conferred to the bimodal crab host C. inversa by attenuating oxidative stress on gill tissues during the animal's emersion periods [61]. Additionally, the sequences of UG2_1 T and UG2_2 encode for several stress-related genes potentially conferring symbiotic advantages during their association with the animal host.…”