Lipid Peroxidation in Peribacteroid Membranes from French-Bean Nodules

Puppo, Alain; Herrada, Gilles; Rigaud, J.

doi:10.1104/pp.96.3.826

Cited by 41 publications

(24 citation statements)

References 30 publications

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“…Measurements of the products of lipid peroxidation in nodules were conducted using the thiobarbituric acid technique (Puppo et al, 1991). Nodules (c. 0n25 g f. wt) were ground with a mortar and pestle with 2 ml of ice-cold 0n1% (w\v) trichloroacetic acid.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Effects of exogenous application and stem infusion of ascorbate on soybean (Glycine max) root nodules

Bashor

Dalton

1999

New Phytologist

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Numerous biochemical and physiological studies have demonstrated the importance of ascorbate (ASC) as a reducing agent and antioxidant in higher plant metabolism. Of special note is the capacity of ASC to eliminate damaging activated oxygen species (AOS) including O # d and H # O # . N # -fixing legume nodules are especially vulnerable to oxidative damage because they contain large amounts of leghaemoglobin which produces AOS through spontaneous autoxidation ; thus, ASC and other components of the ascorbate-reduced glutathione (ASC-GSH) pathway are critical antioxidants in nodules. In order to establish a meaningful correlation between concentrations of ASC and capacity for N # fixation in legume root nodules, soybean (Glycine max) plants were treated with excess ASC via exogenous irrigation or continuous intravascular infusion through needles inserted directly into plant stems. Treatment with ASC led to striking increases in nitrogenase activity (acetylene reduction), nodule leghaemoglobin content, and activity of ASC peroxidase, a key antioxidant enzyme. The concentration of lipid peroxides, which are indicators of oxidative damage and onset of senescence, was decreased in ASC-treated nodules. These results support the conclusion that ASC is critical for N # fixation and that elevated ASC allows nodules to maintain a greater capacity to fix N # over longer periods.

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Section: Biochemical Assaysmentioning

confidence: 99%

Effects of exogenous application and stem infusion of ascorbate on soybean (Glycine max) root nodules

Bashor

Dalton

1999

New Phytologist

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“…These reactions are likely to result in the initiation of lipid peroxidation (as has been observed in other studies where the overall result of the incubation of metLb with PBMs and H 2 O 2 has been investigated) (9,10) and possibly membrane degradation, if the antioxidant molecules present in the membrane are either overwhelmed or depleted.…”

Section: Discussionmentioning

confidence: 94%

“…During the early stages of nodule senescence, the PBM is one of the first structures to be degraded, and previous studies have implicated species derived from Lb in this process (9,10). Thus, the question of the involvement of globin radicals arises.…”

Section: Examination Of the Reaction Of Globin-derived Radicals With mentioning

confidence: 99%

“…Exposure of nodule membrane fractions to a metLb/H 2 O 2 mixture is known to induce oxidation of the lipids with the generation of product materials, although how such a process is initiated remains unclear (9,10). Previous studies have suggested that this lipid peroxidation process might result from (a) reaction of iron ions released from damaged Lb with excess peroxide and hence generation of free hydroxyl radicals which subsequently oxidize lipid molecules (11), (b) direct reaction of the iron(IV)-oxo (ferryl) species with membrane lipids, or (c) direct reaction of the globin radical(s) with membrane lipids and hence transfer of damage from the protein to the fatty acids (cf.…”

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confidence: 99%

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Leghemoglobin-derived Radicals

Moreau

Davies

Mathieu

et al. 1996

Journal of Biological Chemistry

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Reaction of H 2 O 2 with ferric leghemoglobin (metLb, the monomeric, oxygen-carrying, heme protein from root nodules of nitrogen-fixing plants) has been previously shown to generate an iron(IV)-oxo (ferryl) species and at least one protein radical. The latter has been suggested to be a tyrosine-derived phenoxyl radical present at Tyr-133 in the soybean protein and Tyr-138 in the lupin protein. To obtain further information on these protein radicals and their potential interaction with the physiologically important peribacteroid membrane (which surrounds the microsymbiont in vivo), EPR spin trapping studies have been carried out with soybean metLb.Evidence has been obtained for at least two additional protein-derived radicals in addition to the phenoxyl radical; these radicals are transient and reactive in nature. These species are carbon-centered, and at least one is a tertiary species ( ⅐ CR 1 R 2 R 3 ); these radicals may be side chain-or ␣-carbon-derived, their exact sites have not been determined. Some of these radicals are on the protein surface and may be key intermediates in the formation of protein dimers.These radicals have been shown to be capable of reacting with peribacteroid membrane fractions, with the consequent generation of lipid-derived radicals. The formation of such radicals may result in the depletion of membrane antioxidants and the initiation of lipid peroxidation. This transfer of damage from the heme center via the protein surface to neighboring membranes may be of considerable biological significance; the destruction of this membrane is one of the earliest observable events in root nodule senescence and is associated with the loss of nitrogen-fixing activity.

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“…The TBARS assay was modified from Puppo et al (1991). About 50 mg of nodules was frozen in liquid nitrogen and ground to a powder in Eppendorf tubes with a small plastic pestle.…”

Section: Measurements Of Oxidative Damagementioning

confidence: 99%

Physiological Roles of GlutathioneS-Transferases in Soybean Root Nodules

et al. 2009

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Glutathione S-transferases (GSTs) are ubiquitous enzymes that catalyze the conjugation of toxic xenobiotics and oxidatively produced compounds to reduced glutathione, which facilitates their metabolism, sequestration, or removal. We report here that soybean (Glycine max) root nodules contain at least 14 forms of GST, with GST9 being most prevalent, as measured by both real-time reverse transcription-polymerase chain reaction and identification of peptides in glutathione-affinity purified extracts. GST8 was prevalent in stems and uninfected roots, whereas GST2/10 prevailed in leaves. Purified, recombinant GSTs were shown to have wide-ranging kinetic properties, suggesting that the suite of GSTs could provide physiological flexibility to deal with numerous stresses. Levels of GST9 increased with aging, suggesting a role related to senescence. RNA interference studies of nodules on composite plants showed that a down-regulation of GST9 led to a decrease in nitrogenase (acetylene reduction) activity and an increase in oxidatively damaged proteins. These findings indicate that GSTs are abundant in nodules and likely function to provide antioxidant defenses that are critical to support nitrogen fixation.

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Lipid Peroxidation in Peribacteroid Membranes from French-Bean Nodules

Cited by 41 publications

References 30 publications

Effects of exogenous application and stem infusion of ascorbate on soybean (Glycine max) root nodules

Effects of exogenous application and stem infusion of ascorbate on soybean (Glycine max) root nodules

Leghemoglobin-derived Radicals

Physiological Roles of GlutathioneS-Transferases in Soybean Root Nodules

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