Lipid peroxidation induced by ultrasonication in Ehrlich ascitic tumor cells

Hristov, Peter Karakashev; Petrov, Lubomir; Russanov, E

doi:10.1016/s0304-3835(97)00318-2

Cited by 30 publications

(11 citation statements)

References 16 publications

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“…Highly active species such as Å OH induced by ultrasound cavitation are able to change many physiochemical properties of the cell membrane, including the level of membrane lipid peroxide, and cause complex biological responses [35]. In this paper, lipid peroxidation (Fig.…”

Section: Discussionmentioning

confidence: 92%

Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells

Jia

Yuan

Zhang

et al. 2015

Ultrasonics Sonochemistry

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Section: Discussionmentioning

confidence: 92%

Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells

Jia

Yuan

Zhang

et al. 2015

Ultrasonics Sonochemistry

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“…Compared with 'photodynamic therapy', this phenomenon has been termed 'sonodynamic therapy' (SDT) and attracts increasing attention [3][4][5][6][7][8][9][10]. SDT appears to be a promising modality for cancer treatment, for ultrasound can penetrate deep within the tissue and can be focused in a small region of tumor to chemically activate relatively non-toxic molecules thus minimizing undesirable side effects [3].…”

Section: Introductionmentioning

confidence: 99%

FCLA chemiluminescence from sonodynamic action in vitro and in vivo

Xing

Yan

et al. 2002

Cancer Letters

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“…In our experiment, cell membrane changes could be reflected clearly by lipid peroxidation, and morphological observation under scanning electron microscope. MDA assay is probably the most common method for the measurement of lipid peroxidation [32,33] . The level of MDA reflects the degree of cell damage caused by oxygen free radicals indirectly, our results suggested the level of lipid peroxidation in the ultrasound plus 100 g/ml Hp treatment group was significantly enhanced (p !…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

Wang

Wang²,

Liu³

et al. 2008

Chemotherapy

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It was the aim of this study to investigate the ultrasonically induced cytotoxic effect of hematoporphyrin (Hp) on isolated sarcoma 180 (S180) cells and to explore the potential biological mechanism of this action. S180 tumor cells suspended in air-saturated phosphate-buffered saline (pH 7.2) were exposed to ultrasound at 1.6 MHz in a standing wave mode for up to 90 s with and without 100 µg/ml Hp. The intracellular Hp concentration was evaluated to determine the optimum timing of ultrasound exposure after its administration with a fluorescence spectrophotometer based on the standard curve. Cell viability was determined by the trypan blue exclusion test. The morphological changes of S180 cells induced by ultrasonic irradiation were evaluated by scanning electron microscope and transmission electron microscope observation. The participation of lipid peroxidation products in the cell damage process was investigated by the malon aldehyde content test. Our experiments suggested that an incubation time of 45 min after the addition of 100 µg/ml Hp was to be chosen as the best time for ultrasound exposure in vitro. The rate of ultrasonically induced cell damage was enhanced by the increase in ultrasound intensity and exposure time in the presence and absence of Hp. At an ultrasound intensity not less than 3 W/cm² and an irradiation time not less than 60 s, a significant synergistic effect of ultrasound combined with Hp was observed, while no cell damage was observed with 100 µg/ml Hp alone. The malon aldehyde content test showed that the lipid peroxidation level significantly increased after sonodynamic therapy treatment. Scanning electron microscope and transmission electron microscope observation indicated that the changes in cell ultra-structure such as cell membrane destruction, mitochondria swelling and chromatin condensation are important factors that induced cell damage in S180 tumor cells. The results indicate that the biological mechanism might be involved in mediating the killing effect on S180 cells, and the ultra-structural changes may account for cell destruction induced by sonodynamic therapy treatment in our experiment mode.

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Lipid peroxidation induced by ultrasonication in Ehrlich ascitic tumor cells

Cited by 30 publications

References 16 publications

Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells

Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells

FCLA chemiluminescence from sonodynamic action in vitro and in vivo

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

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