Lipid Peroxidation Inhibitory Activity In vitro of Mezzetia parviflora Becc. Wood Bark Polar extract

Abstract

Introduction:The wood bark of Mezzetia parviflora Becc, has long served as one of the most important traditional herbal medicine sources in Buton Regency, Southeast Sulawesi. M. parviflora extracts were rich in polyphenols. This study was aimed to explore the lipid peroxidation inhibitory activity of polar extract of M. parviflora. Methods: The polar extract is the result of ethanol extract partition solved in acetone. The extract will keep polar components which are insoluble in acetone. Assayed methods applied are ß-carotene bleaching inhibition, thiobarbituric acid reactive substance (TBARS) measurement, and continuous monitoring of conjugated dienes formation in LDL. Results: M. parviflora extract inhibit ß-carotene/ linoleic acid oxidation, showed by IC50 value of 15.83 µg/ml in 30th minute; but the potency will be reduced to IC50 value of 111.19 µg/ml and 225.07 µg/ml after the 60th and 120th minute of incubation. M. parviflora extract inhibit MDA formation as for linoleic acid peroxidation product until the third day; at 20, 40, 60, 80 and 100 µg/ml inhibit MDA formation as many as 29.16 ± 2.41%, 4.24% ± 43.27, 54.08 ± 2.87%, 59.88 ± 1.90%, and 69.75 ± 2.32%, respectively. M. parviflora extract at 50 µg/ml can inhibit LDL-oxidation induced by CuSO4, performed by LDL-oxidation lag-time elongation until 70 minutes, similar ability was performed by epigallocathecin gallate at 5 µg/ml. Conclusions: M. parviflora extract expressed relatively strong protection against lipid and LDL oxidation which can serve as the scientific basis of its development as a remedy for various diseases caused by lipid peroxidation.

ANTI-INFLAMATORY AND ANTI-LIPID PEROXODATION POTENTIALS OF SALVIA CHUDAEI BATT. et TRAB. AND LAVANDULA ANTINEAE MAIRE: ENDEMIC PLANTS FROM ALGERIAN SAHARA

“…Lipid peroxidation is caused by the generation of hydroxyl and superoxide radicals, leading to the formation of peroxy radicals that eventually propagate the chain reaction in lipids. Therefore, the presence of natural antioxidant molecules such as phenolic compounds could prevent lipid peroxidation by naturalizing radical peroxides through different modes of action including free radical scavenging, electron transfer, radical addition and radical recombination 21,22 .…”

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“…These free radicals or reactive oxygen species are the major cause of lipid deterioration and are easily prone to lethal peroxidative modification. Hence based on the peroxidation process, a molecule of Malan oldehyde (MDA) interacts with two molecules of Thiobarbituric acid (TBA) to form malanoldehyde TBA complex hence changing to red color, which can be absorbed in 500 nm 32 . The percentage of inhibition of lipid peroxidation by the standard compound at 100 µg is 90.6% and whereas both B1 and B2 show the inhibition at 83 % at highest concentration compared to the rest two lower concentration.…”

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

“…Inhibition of low-density lipoprotein (LDL) oxidation based on the antioxidant effect on the peroxidation of linoleic acid or LDL induced by Cu(II) or AAPH [30,31];…”