Lipid-polymer hybrid nanoparticles: Development & statistical optimization of norfloxacin for topical drug delivery system

Abstract

Poly lactic acid is a biodegradable, biocompatible, and non-toxic polymer, widely used in many pharmaceutical preparations such as controlled release formulations, parenteral preparations, surgical treatment applications, and tissue engineering. In this study, we prepared lipid-polymer hybrid nanoparticles for topical and site targeting delivery of Norfloxacin by emulsification solvent evaporation method (ESE). The design of experiment (DOE) was done by using software to optimize the result, and then a surface plot was generated to compare with the practical results. The surface morphology, particle size, zeta potential and composition of the lipid-polymer hybrid nanoparticles were characterized by SEM, TEM, AFM, and FTIR. The thermal behavior of the lipid-polymer hybrid nanoparticles was characterized by DSC and TGA. The prepared lipid-polymer hybrid nanoparticles of Norfloxacin exhibited an average particle size from 178.6 ± 3.7 nm to 220.8 ± 2.3 nm, and showed very narrow distribution with polydispersity index ranging from 0.206 ± 0.36 to 0.383 ± 0.66. The surface charge on the lipid-polymer hybrid nanoparticles were confirmed by zeta potential, showed the value from +23.4 ± 1.5 mV to +41.5 ± 3.4 mV. An Antimicrobial study was done against Staphylococcus aureus and Pseudomonas aeruginosa, and the lipid-polymer hybrid nanoparticles showed potential activity against these two. Lipid-polymer hybrid nanoparticles of Norfloxacin showed the %cumulative drug release of 89.72% in 24 h. A stability study of the optimized formulation showed the suitable condition for the storage of lipid-polymer hybrid nanoparticles was at 4 ± 2 °C/60 ± 5% RH. These results illustrated high potential of lipid-polymer hybrid nanoparticles Norfloxacin for usage as a topical antibiotic drug carriers.

Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications

“…The morphology of the prepared NPs as evaluated via SEM reveled spherical structures with smooth surface and fine size distribution for PLA/PVA-NPs (Figure 2a), while under the same settings PLA/LEC-NPs revealed some distinctive spherical structures embedded in a fused background (Figure 2b). This morphology is typical of similar lipid-polymer hybrid nanoparticles [30] and may be justified by the fact that due to the presence of LEC excess of water is entrapped in their structure, thus hindering focusing of electron beam on the sample. This fact may suggest existence of different structure between PLA/PVA-NPs and PLA/LEC-NPs and may justify the reduced stability exhibited by the later.…”

Lipid Polymer Hybrid Nanoparticles: A Novel Approach for Drug Delivery

“…These nanoparticles have shown antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa and could be used for the treatment of burn wound and topical infections. SLNs have shown 89.72% drug release after 24 h and passed the skin irritation and stability tests [43].…”

Therapeutic lipid-coated hybrid nanoparticles against bacterial infections

“…In general, fabrication of LCHNPs can be achieved through two distinctive approaches: (1) a multi-step process whereby the nanoparticle core and lipid shell are prepared separately; [43][44][45][46] or (2) a single-step process. [47][48][49][50] The latter is oen obtained through a one-pot emulsionsolvent-evaporation (ESE) or through a nanoprecipitation method. In this review, only the most widely employed approaches will be discussed, along with several recent representative examples of each fabrication method.…”