Lipid production from hemicellulose hydrolysate and acetic acid by Lipomyces starkeyi and the ability of yeast to metabolize inhibitors

Xavier, Michelle da Cunha Abreu; Coradini, Alessandro L V; Deckmann, Ana Carolina; Franco, Telma Teixeira

doi:10.1016/j.bej.2016.11.007

Cited by 84 publications

(31 citation statements)

References 57 publications

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“…The capability of L. starkeyi to metabolize glucose, arabinose, lactic acid and acetic acid present in SBP hydrolysates was determined by analysing the concentration of these compounds at different time points. As previously observed [24,27,28], L. starkeyi is able to co-consume glucose and acetic acid, but arabinose and lactic acid were not assimilated throughout the cultivation time ( Figure 2B).…”

Section: Growth and Lipid Accumulation By L Starkeyi In Sbp Hydrolyssupporting

confidence: 84%

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Martani

Maestroni

Torchio

et al. 2020

Preprint

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Background: Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required.Results: In this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2 %, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2 %) in the presence of 6 % molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2 %, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. Conclusions: This study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor.

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Section: Growth and Lipid Accumulation By L Starkeyi In Sbp Hydrolyssupporting

confidence: 84%

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Martani

Maestroni

Torchio

et al. 2020

Preprint

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“…is is due to its richness in hemicellulose which is mainly affected by acid treatment and also due to its low lignin content [17]. However, sugarcane bagasse, date palm leaf, Posidonia balls, and almond shell do not exceed 28 g/L of liberated reducing sugars.…”

Section: Selection Of Lignocellulosic By-productmentioning

confidence: 99%

“…Compared with other yeast of Rhodotorula genus, the lipid content of Y-MG1 [8,19]. Cultivation of C. starkeyi on bagasse hydrolysate under different culture modes had resulted in lipid yields not exceeding 28% [17]. In addition, C. tropicalis and T. cutaneum CX1 cultivated on palm empty fruit and corn stover hydrolysate, respectively, produce only 1.6 and 3.1 g/L of lipids using batch bioreactor culture [53,54].…”

Section: Carbon Sourcementioning

confidence: 99%

“…ese toxic compounds exert an inhibitory effect on the growth of microorganisms and the cost of their removal will significantly reduce the profitability of the bioconversion process [16]. In order to increase the bioconversion rate of lignocellulosic biomass, several detoxification processes have been developed to eliminate and reduce toxic compounds derived from the hydrolysis step [17]. e overliming using the calcium hydroxide and the combination of overliming and adsorption using the activated charcoal or the amberlite resin are the most studied of chemical and physical detoxification methods [1,9].…”

Section: Introductionmentioning

confidence: 99%

“…All of these detoxification treatments lead to increase the cost of the bioconversion process, so other alternatives must be found to enhance the lipid yield production [18]. Several previous works have studied many kinds of lignocellulosic materials with different hydrolysis processes for lipids production such as corn stalk, bagasse, corncob, and wheat straw [8,[17][18][19]. e rate of lipid production depends on the nature of LB as a supplier of sugars and the potential of microorganisms that will convert sugars into energy molecules.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis

Ayadi

Belghith

Gargouri

et al. 2019

BioMed Research International

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The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis. On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content. This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose. Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid. An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes. The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material. Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements. Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively. The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran. The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.

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Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose‐hydrolysate and crude glycerol

Passoth

Brandenburg

Chmielarz

et al. 2023

Yeast

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Microbial lipids produced from lignocellulose and crude glycerol (CG) can serve as sustainable alternatives to vegetable oils, whose production is, in many cases, accompanied by monocultures, land use changes or rain forest clearings. Our projects aim to understand the physiology of microbial lipid production by oleaginous yeasts, optimise the production and establish novel applications of microbial lipid compounds. We have established methods for fermentation and intracellular lipid quantification. Following the kinetics of lipid accumulation in different strains, we found high variability in lipid formation even between very closely related oleaginous yeast strains on both, wheat straw hydrolysate and CG. For example, on complete wheat straw hydrolysate, we saw that one Rhodotorula glutinis strain, when starting assimilating D‐xylosealso assimilated the accumulated lipids, while a Rhodotorula babjevae strain could accumulate lipids on D‐xylose. Two strains (Rhodotorula toruloides CBS 14 and R. glutinis CBS 3044) were found to be the best out of 27 tested to accumulate lipids on CG. Interestingly, the presence of hemicellulose hydrolysate stimulated glycerol assimilation in both strains. Apart from microbial oil, R. toruloides also produces carotenoids. The first attempts of extraction using the classical acetone‐based method showed that β‐carotene is the major carotenoid. However, there are indications that there are also substantial amounts of torulene and torularhodin, which have a very high potential as antioxidants.

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Lipid production from hemicellulose hydrolysate and acetic acid by Lipomyces starkeyi and the ability of yeast to metabolize inhibitors

Cited by 84 publications

References 57 publications

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis

Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose‐hydrolysate and crude glycerol

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