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Research background. Microbial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated Aspergillus carbonarius PC-4 strain was used to optimize the β-fructofuranosidase production under a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars. Experimental approach. Optimization of nutritional components of culture medium was carried out using Simplex Lattice mixture design for 72 h and 120 h at 28 ˚C. One-Factor-at-a-time methodology was used to optimize the physicochemical parameters. Crude enzyme was used for sucrose hydrolysis at different concentrations. Results and conclusions. The optimized condition of enzyme production was pineapple crown waste (1.3 %, m/V) and yeast extract (0.3 %, m/V) for 72 h (9.4 U/mL), obtaining R² 91.85 %, R² adjusted 85.06 %, highest F value (13.52) and low p-value (0.003). One-factor-at-a-time used for optimizing the physicochemical conditions showed optimum temperature (20 ˚C), pH (5.5), agitation (180 rpm) and time-course (72 h) with an increase of 3.0-folds for enzyme production. The invertase-induced sucrose hydrolysis showed the maximum yield (3,451.7 µmols of reducing sugars) using 10 % of initial sucrose concentration. Higher sucrose concentrations caused inhibition of invertase activity, possibly due to saturation of substrate or formation of sucrose aggregates making it difficult for the enzyme to access sucrose molecules within the created clusters. So, a cost-effective method was developed for the invertase production using agroindustrial waste and the enzyme produced can be used efficiently for inverted sugar production at high sucrose concentration. Novelty and scientific contribution. This study presents an efficient utilization of pineapple crown wastes to produce invertase by a newly isolated Aspergillus carbonarius PC-4 strain. This enzyme exhibited a good potential for invert sugar production at high initial sucrose concentration, which is interesting for industrial applications.