Lipid production in Porphyridium cruentum grown under different culture conditions

Oh, Sung Ho; Han, Jae Gun; Kim, Young; Ha, Ji Won; Kim, Seung Seop; Jeong, Myoung Hoon; Jeong, Hyang Suk; Kim, Na Young; Cho, Jeong Sub; Yoon, Won Byong; Lee, Shin Young; Kang, Do Hyung; Lee, Hyeon Yong

doi:10.1016/j.jbiosc.2009.05.020

Cited by 82 publications

(46 citation statements)

References 10 publications

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“…4), revealing that higher aeration rate played a positive role on ARA biosynthesis. Maximum volumetric content of TFAs and ARA was both obtained at cultivation with high aeration rate conditions possibly due to the constant supply of CO 2 ; which has been proven to play an important role in elevating the lipid content in algal cells (Oh et al 2009;Akimoto et al 1998). In addition, CO 2 was used for de novo fatty acid synthesis (Jiang et al 2011).…”

Section: Resultsmentioning

confidence: 97%

“…Algae cells were cultivated in 1 L flasks containing 500 mL medium at 25 °C under continuous light illumination in a photoincubator. Four culture media reported to enhance P. cruentum growth, including Jones' ASW medium (Jones et al 1963), KOCK medium (Koch 1952), Pringsheim medium II (Ernest and Pringsheim 1949), and F/2 medium (Oh et al 2009), were screened for biomass production and fatty acids/ARA accumulation. The pH of the mediums was adjusted to 7.6 by Tris-HCl buffer.…”

Section: Culture Systemmentioning

confidence: 99%

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Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

Jiao

Chang

et al. 2016

Bioresour. Bioprocess.

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Objectives: This study investigated the effect of aeration rate and light intensity on biomass production and total fatty acids (TFA) accumulation by Porphyridium purpureum. The red microalgae is also known to accumulate considerable amount of arachidonic acid (ARA). Results:In artificial seawater medium, the highest yield of TFA (473.44 mg/L) was obtained with the aeration rate of 3 L/min and light intensity of 165 µmol/m 2 s, whilst the highest yield of ARA (115.47 mg/L) was achieved with the aeration rate of 3 L/min and light intensity of 110 µmol/m 2 s. It was found that higher aeration rate led to more biomass and TFA/ARA production. However, higher light intensity could contribute to biomass accumulation, but it was adverse for TFA and ARA biosynthesis. Conclusion:By optimizing two operating factors (i.e., light intensity and aeration rate), TFA and ARA production by P. purpureum was significantly improved. This research provides a potential alternative means for producing ARA.

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Section: Resultsmentioning

confidence: 97%

Section: Culture Systemmentioning

confidence: 99%

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

Jiao

Chang

et al. 2016

Bioresour. Bioprocess.

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“…The microalga Porphyridium sp. (Rhodophyta) is a potential source for several products, such as fatty acids and lipids [1,2], pigments [3], and cell-wall polysaccharides [4]. These polysaccharides are sulphated and their structure gives rise to some unique properties that could lead to a broad range of industrial and pharmaceutical applications [5].…”

Section: Introductionmentioning

confidence: 99%

Effects of nitrogen on growth and carbohydrate formation in Porphyridium cruentum

2013

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The microalga Porphyridium cruentum (Rhodophyta) has several industrial and pharmaceutical uses, especially for its polysaccharide production. This study aimed to investigate the influence of nitrogen levels as reflected by altered N:P ratios on the production and content of biomass and carbohydrate. N:P molar ratios were altered in batch cultures to range from 1.6 to 50 using the Redfield ratio of 1:16 as reference. Algal growth (estimated as final cell number, biomass concentration and maximum specific growth rate) was negatively affected at low N:P ratios. The optimal N:P ratio for growth was identified at 35–50, with specific growth rates of 0.19 day−1 and maximum cell concentrations of 59·108 cells L−1 and 1.2 g dry weight of biomass L−1. In addition, variation in cell size was seen. Cells with larger diameters were at higher N:P ratios and smaller cells at lower ratios. The cellular carbohydrate content increased under reduced nitrogen availability. However, because accumulation was moderate at the lowest N:P ratio, 0.4 g per g dry weight biomass compared to 0.24 at the Redfield ratio of 16:1, conditions for increased total carbohydrate formation were identified at the N:P ratios optimal for growth. Additionally, carbohydrates were largely accumulated in late exponential to stationary phase.

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“…Downstream processing studies have been conducted to evaluate the efficiency of processing algae or "algae oil," without including relevant information about the algal species, lipid content, or oil composition [93,94]. Since growth rate, lipid content, and lipid composition can vary widely depending on species or growth conditions [1,21,41,[97][98][99][100][101][102][103][104][105], the processing efficiencies determined for one alga or one composition of "algae oil" may differ from those associated with different algae or oil compositions. The resources required for production may also vary depending on species [58].…”

Section: Inclusive Resultsmentioning

confidence: 99%

A Framework to Report the Production of Renewable Diesel from Algae

et al. 2010

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Recently, algae have received significant interest as a potential feedstock for renewable diesel (such as biodiesel), and many researchers have attempted to quantify this potential. Some of these attempts are less useful because they have not incorporated specific values of algal lipid content, have not included processing inefficiencies, or omitted processing steps required for renewable diesel production. Furthermore, the associated energy, materials, and costs requirements are sometimes omitted. The accuracy and applicability of these estimates can be improved by using data that are more specific, including all relevant information for renewable diesel production, and by presenting information with more relevant metrics. To determine whether algae are a viable source for renewable diesel, three questions that must be answered are (1) how much renewable diesel can be produced from algae, (2) what is the financial cost of production, and (3) what is the energy ratio of production? To help accurately answer these questions, we propose an analytical framework and associated nomenclature system for characterizing renewable diesel production from algae. The three production pathways discussed in this study are the transesterification of extracted algal lipids, thermochemical conversion of algal biomass, and conversion of secreted algal oils. The nomenclature system is initially presented from a top-level perspective that is applicable to all production pathways for renewable diesel from algae. Then, the nomenclature is expanded to characterize the production of renewable diesel (specifically, biodiesel) from extracted algal lipids in detail (cf. Appendix 2). The analytical framework uses the presented nomenclature system and includes three main principles: using appropriate reporting metrics, using symbolic notation to represent unknown values, and presenting results that are specific to algal species, growth conditions, and product composition.

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Lipid production in Porphyridium cruentum grown under different culture conditions

Cited by 82 publications

References 10 publications

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

Effects of nitrogen on growth and carbohydrate formation in Porphyridium cruentum

A Framework to Report the Production of Renewable Diesel from Algae

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