BIOFLOCCULATION FOR CONTROL OF WASTEWATER POND MICROALGAE DANIEL THOMAS FROSTWastewater treatment pond effluents often have high concentrations of total suspended solids (TSS) consisting mainly of colloidal microalgal cells. For many pond systems, TSS concentrations approach or exceed discharge limits. Conventional algae removal methods, such as coagulation followed by dissolved air flotation, negate much of the cost and simplicity advantages of pond systems. Bioflocculation, the natural agglomeration of cells, followed by sedimentation is a low-cost method to remove pond TSS, but one that is unreliable in current practice. In the present research, bioflocculation experiments were conducted at a central California pilot-scale high rate pond (HRP) facility, consisting of four identical 580-gallon HRPs. HRPs are shallow, paddle wheel-mixed raceways that provide more rapid treatment than conventional ponds. Settleability of the HRP TSS was compared using Imhoff cones.The experiments related to two aspects of bioflocculation -organic loading effects and inoculation with flocculent biomass, such as return activated sludge (RAS). For the first, analysis of data from HRPs around the world shows that high ratios of influent soluble organic loading-to-Pond TSS (sBOD in /TSS pond , where sBOD refers to the 5-day soluble biochemical oxygen demand or BOD 5 ) correlate to lower TSS concentrations in the effluent of subsequent algae settling units (TSS su The experiments indicated that RAS inoculation provided multiple benefits: (1) improved bioflocculation and settling, (2) apparent stimulation of autotrophic productivity, (3) improved BOD removal, and in some cases (4) improved ammonium-N removal.Despite higher pond TSS concentrations in the inoculated ponds than in the controls, the inoculated pond effluents settled to lower mean TSS concentrations (21 mg/L versus 34 mg/L). These settled TSS concentrations corresponded to TSS removal efficiencies of 92% for the inoculated ponds and 74% for the control ponds. The autotrophic growth in the inoculated ponds was as much as 15 g/m 2 /day higher than in the control ponds. In one experiment, inoculation seemed responsible for higher sBOD removal (91% versus 78%). After settling, the total BOD 5 for the inoculated ponds was 16 mg/L compared to 23 mg/L for the control ponds. Finally in two experiments, higher ammonium-N removal was observed in the inoculated ponds than in the control ponds (83% versus 62%). These RAS-induced benefits, in addition to better understanding of the effects of organic loading on bioflocculation, imply potentially significant improvements to the feasibility of HRPs as a reliable wastewater treatment technology.vi ACKNOWLEDGMENTS