Lipid profiling of barley root in interaction with Fusarium macroconidia

Reyna, Matias; Margutti, Micaela Peppino; Villasuso, Ana Laura

doi:10.1016/j.envexpbot.2019.06.001

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…The contribution of HvLPCAT and HvPLA to the acyl-editing of PC was assessed on barley roots exposed to Fusarium macroconidia for 30 or 60 min, and their respective controls. 4…”

Section: Course Designmentioning

confidence: 99%

“…To make the analysis less overwhelming for the students, the dataset was condensed by selecting phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) lipids and their molecular species, a total of 360 inputs. The contribution of HvLPCAT and HvPLA to the acyl‐editing of PC was assessed on barley roots exposed to Fusarium macroconidia for 30 or 60 min, and their respective controls 4 …”

Section: Course Designmentioning

confidence: 99%

“…The relative expression of related genes was also included. Our research group had prior experience using the platform and following the pipeline to analyze lipidomics data from barley tissues 3–5 . For this reason, we considered it a useful tool on which to base the lesson.…”

Section: Introductionmentioning

confidence: 99%

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Using MetaboAnalyst to introduce undergraduates to lipidomic analysis and lipid remodeling in barley roots

Reyna

Margutti

Vilchez

et al. 2023

Biochem Molecular Bio Educ

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Lipidomics is a discipline that focuses on the identification and quantification of lipids. Although a part of the larger omics field, lipidomics requires specific approaches for the analysis and biological interpretation of datasets. This article presents a series of activities for introducing undergraduate microbiology students to lipidomic analysis through tools from the web‐based platform MetaboAnalyst. The students perform a complete lipidomic workflow, which includes experiment design, data processing, data normalization, and statistical analysis of molecular phospholipid species obtained from barley roots exposed to Fusarium macroconidia. The input data are provided by the teacher, but students also learn about the methods through which they were originally obtained (untargeted liquid chromatography coupled with mass spectrometry). The ultimate aim is for students to understand the biological significance of phosphatidylcholine acyl editing. The chosen methodology allows users who are not proficient in statistics to make a comprehensive analysis of quantitative lipidomic datasets. We strongly believe that virtual activities based on the analysis of such datasets should be incorporated more often into undergraduate courses, in order to improve students' data‐handling skills for omics sciences.

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“…The contribution of HvLPCAT and HvPLA to the acyl-editing of PC was assessed on barley roots exposed to Fusarium macroconidia for 30 or 60 min, and their respective controls. 4…”

Section: Course Designmentioning

confidence: 99%

Section: Course Designmentioning

confidence: 99%