Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues

Liaw, Lucy; Prudovsky, Igor; Koza, Robert A.; Anunciado-Koza, Rea Victoria P.; Siviski, Matthew E.; Lindner, Volkhard; Friesel, Robert; Rosen, Clifford J.; Baker, Paul R. S.; Simons, Brigitte; Vary, Calvin P.H.

doi:10.1002/jcb.25522

Cited by 41 publications

(48 citation statements)

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“…For example, the lipidomes of cultured undifferentiated cells are strikingly different than the lipidomes of the respective differentiated cells, and include characteristic bio-markers for each stage of the lineage. 24 Despite general differences between cell lines and primary cells, PCA plots indicate a similar trajectory in how lipid profiles change during differentiation. 24 Interestingly, comparison of the lipidomes of in vitro differentiated adipocytes to dissected mouse adipose tissues show similarities together with dissimilarities.…”

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 98%

“…24 Despite general differences between cell lines and primary cells, PCA plots indicate a similar trajectory in how lipid profiles change during differentiation. 24 Interestingly, comparison of the lipidomes of in vitro differentiated adipocytes to dissected mouse adipose tissues show similarities together with dissimilarities. For example, TG composition of in vitro differentiated white cells is similar to that of WAT, whereas their PL composition shows no resemblance.…”

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 98%

“…For example, TG composition of in vitro differentiated white cells is similar to that of WAT, whereas their PL composition shows no resemblance. 24 Another example is that in vitro differentiation of brown-like cells is relatively similar to BAT in terms of SM composition. 24 However, the lipidomes of adipocytes differentiated from pre-adipocyte cell lines are quite different in comparison to the lipidomes of adipocytes differentiated from mesenchymal stem cells.…”

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

“…24 Another example is that in vitro differentiation of brown-like cells is relatively similar to BAT in terms of SM composition. 24 However, the lipidomes of adipocytes differentiated from pre-adipocyte cell lines are quite different in comparison to the lipidomes of adipocytes differentiated from mesenchymal stem cells. 24 Collectively, cultured adipocytes do not exactly resemble a freshly obtained adipose tissue.…”

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

“…24 However, the lipidomes of adipocytes differentiated from pre-adipocyte cell lines are quite different in comparison to the lipidomes of adipocytes differentiated from mesenchymal stem cells. 24 Collectively, cultured adipocytes do not exactly resemble a freshly obtained adipose tissue. Those differences in lipid profiles might be a result of diverse signals driving lipogenesis in vivo, the presence of other cell types in the tissue apart from adipocytes, and the different fatty acids supplemented in vitro as compared with the fatty acids obtained by food intake.…”

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

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Form(ul)ation of adipocytes by lipids

Lapid

Graff

2017

Adipocyte

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Lipids have the potential to serve as bio-markers, which allow us to analyze and to identify cells under various experimental settings, and to serve as a clinical diagnostic tool. For example, diagnosis according to specific lipids that are associated with diabetes and obesity. The rapid development of mass-spectrometry techniques enables identification and profiling of multiple types of lipid species. Together, lipid profiling and data interpretation forge the new field of lipidomics. Lipidomics can be used to characterize physiologic and pathophysiological processes in adipocytes, since lipid metabolism is at the core of adipocyte physiology and energy homeostasis. A significant bulk of lipids are stored in adipocytes, which can be released and used to produce energy, used to build membranes, or used as signaling molecules that regulate metabolism. In this review, we discuss how exhaust of lipidomes can be used to study adipocyte differentiation, physiology and pathophysiology.

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Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 98%

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 98%

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

Section: Lipidomics Of Adipocyte Differentiationmentioning

confidence: 99%

See 3 more Smart Citations

Form(ul)ation of adipocytes by lipids

Lapid

Graff

2017

Adipocyte

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Spatial Recruitment of Cardiolipins in Inguinal White Adipose Tissue after Cold Stimulation is Independent of UCP1

Dieckmann

Maurer

Kleigrewe

2021

Euro J Lipid Sci & Tech

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Brown and brite adipocytes are unique for uncoupling protein 1 (UCP1) dependent non‐shivering thermogenesis (NST) induced by cold exposure. Several lipid species are associated with NST in brown and white adipose tissues (WAT). Studies investigating this association rely on the analysis of whole organ homogenates, or pre‐adipocytes differentiated in vitro. These approaches do not account for the regional heterogeneity of WAT. The authors aimed to characterize the spatial lipid composition of WAT in an in vivo context to identify and validate lipids colocalized with UCP1. Therefore, matrix‐assisted laser desorption ionization mass spectrometry imaging (MALDI‐MSI), high‐resolution mass spectrometry, and immunohistochemistry are applied on sections of inguinal WAT from UCP1 knockout and wildtype mice acclimatized to cold. The authors identified spatial overlap of cardiolipins and diacylglycerols in UCP1 positive regions, and triacylglycerols as the main lipid class characteristic for UCP1 negative regions within inguinal WAT. Investigation of UCP1 knockout and wildtype mice housed at room temperature or acclimatized to cold demonstrated that cardiolipins content in WAT is increased upon cold stimulation, independent of UCP1. In summary, a MALDI‐MSI‐based approach is introduced to identify lipids associated with thermogenic adipocytes in adipose tissues and demonstrate a regional cold‐dependent upregulation of cardiolipins independent of UCP1. Practical application: It is demonstrated that MALDI‐MSI is a valuable tool to investigate the spatial distribution of lipids in WAT. Consequently, it is an important complement to LC‐MS/MS analysis of whole tissue homogenates. In combination with immunohistochemistry, it allows the straightforward examination of potential interactions between structural and metabolic traits, as demonstrated by spatial colocalization of UCP1 expression with specific lipid species in the current study. As a major advantage, this enables analyses on subsets of the same sample combining different analytic approaches, thereby potentially reducing the number of experimental animals.

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The skeletal cell‐derived molecule sclerostin drives bone marrow adipogenesis

Fairfield

Falank

Harris

et al. 2017

Journal Cellular Physiology

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125

121

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The bone marrow niche is a dynamic and complex microenvironment that can both regulate, and be regulated by the bone matrix. Within the bone marrow (BM), mesenchymal stromal cell (MSC) precursors reside in a multi-potent state and retain the capacity to differentiate down osteoblastic, adipogenic, or chondrogenic lineages in response to numerous biochemical cues. These signals can be altered in various pathological states including, but not limited to, osteoporotic-induced fracture, systemic adiposity, and the presence of bone-homing cancers. Herein we provide evidence that signals from the bone matrix (osteocytes) determine marrow adiposity by regulating adipogenesis in the bone marrow. Specifically, we found that physiologically relevant levels of Sclerostin (SOST), which is a Wnt-inhibitory molecule secreted from bone matrix-embedded osteocytes, can induce adipogenesis in 3T3-L1 cells, mouse ear- and BM-derived MSCs, and human BM-derived MSCs. We demonstrate that the mechanism of SOST induction of adipogenesis is through inhibition of Wnt signaling in pre-adipocytes. We also demonstrate that a decrease of sclerostin in vivo, via both genetic and pharmaceutical methods, significantly decreases bone marrow adipose tissue (BMAT) formation. Overall, this work demonstrates a direct role for SOST in regulating fate determination of BM-adipocyte progenitors. This provides a novel mechanism for which BMAT is governed by the local bone microenvironment, which may prove relevant in the pathogenesis of certain diseases involving marrow adipose. Importantly, with anti-sclerostin therapy at the forefront of osteoporosis treatment and a greater recognition of the role of BMAT in disease, these data are likely to have important clinical implications.

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Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues

Cited by 41 publications

References 50 publications

Form(ul)ation of adipocytes by lipids

Form(ul)ation of adipocytes by lipids

Spatial Recruitment of Cardiolipins in Inguinal White Adipose Tissue after Cold Stimulation is Independent of UCP1

The skeletal cell‐derived molecule sclerostin drives bone marrow adipogenesis

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