2021
DOI: 10.3390/ijms221810085
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Lipid Self-Assemblies under the Atomic Force Microscope

Abstract: Lipid model membranes are important tools in the study of biophysical processes such as lipid self-assembly and lipid–lipid interactions in cell membranes. The use of model systems to adequate and modulate complexity helps in the understanding of many events that occur in cellular membranes, that exhibit a wide variety of components, including lipids of different subfamilies (e.g., phospholipids, sphingolipids, sterols…), in addition to proteins and sugars. The capacity of lipids to segregate by themselves int… Show more

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Cited by 5 publications
(4 citation statements)
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“…Two main hypotheses were proposed [48]: (i) an elastic compression of the bilayer until the force achieves a critical point where it is broken as a whole (i.e., in a single step), and (ii) a sequential model where, after an initial bilayer compression, the tip encounters different parts of the bilayer, which act as distinct 'layers' or barriers, and the tip advances through them step by step. The classic approach leaned towards the first hypothesis because most of the AFM bilayer breakthrough events were apparently in a single step, with some exceptions caused by bilayer uncoupling of the leaflets [24,49,50]. However, recent reports and the use of advanced force spectroscopy techniques (such as force-clamp spectroscopy) point to a sequential model even in the simplest 'coupled' models [51].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Two main hypotheses were proposed [48]: (i) an elastic compression of the bilayer until the force achieves a critical point where it is broken as a whole (i.e., in a single step), and (ii) a sequential model where, after an initial bilayer compression, the tip encounters different parts of the bilayer, which act as distinct 'layers' or barriers, and the tip advances through them step by step. The classic approach leaned towards the first hypothesis because most of the AFM bilayer breakthrough events were apparently in a single step, with some exceptions caused by bilayer uncoupling of the leaflets [24,49,50]. However, recent reports and the use of advanced force spectroscopy techniques (such as force-clamp spectroscopy) point to a sequential model even in the simplest 'coupled' models [51].…”
Section: Discussionmentioning
confidence: 99%
“…The present contribution deals with the physical properties of RBC mem-branes in normal and obese adults. The two main techniques applied are atomic force microscopy (AFM) in the force spectroscopy mode, which allows the micromechanical measurement of penetration forces [24,25], and fluorescence anisotropy, which provides information on membrane lipid order [26]. The probe used in fluorescence studies has been trimethylammonium diphenylhexatriene (TMA-DPH) that, in contrast to the more commonly used DPH, becomes located and provides information on the interfacial bilayer region between the phospholipid hydrophobic chains and polar head groups [27].…”
Section: Introductionmentioning
confidence: 99%
“…In this way, the formation of bilayers that did not cover the entire substrate surface was made possible. These lipid-depleted areas allowed for measuring bilayer thicknesses and performing proper controls for force-spectroscopy measurements [ 31 ].…”
Section: Discussionmentioning
confidence: 99%
“…This information is important to deepen our understanding of protein functions and disease mechanisms [ 218 ]. Recently, a comprehensive review summarized data on the application of AFM in membrane biophysics, especially in the study of model membranes, lipid-protein interactions, and the formation of Aβ42 fibrils ([ 219 ] and references therein). AFM can also be used to image individual proteins and analyze their structural characteristics.…”
Section: Atomic Force Microscopy: Morphometric and Nanomechanical Par...mentioning
confidence: 99%