Lipid Thermotropic Transitions in Human Stratum Corneum

Golden, Guia M.; Guzek, Donald B.; Harris, Richard R.; McKie, James E; Potts, Russell O.

doi:10.1111/1523-1747.ep12285373

Cited by 289 publications

(155 citation statements)

References 10 publications

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“…T 1 is assigned to lipid structure transformation from lamellar to the disordered, and T 2 is assigned to a protein-associated lipid transition from gel to liquid form [28]. Other previously reported transition peaks at 35−40 °C and 105−120 °C were not seen here, in part because the stratum corneum samples were desiccated after treatment [29,30].…”

Section: Differential Scanning Calorimetrymentioning

confidence: 78%

Transdermal delivery enhanced by magainin pore-forming peptide

Kim

Ludovice

Prausnitz

2007

Journal of Controlled Release

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In this study we tested the hypothesis that magainin, a peptide known to form pores in bacterial cell membranes, can increase skin permeability by disrupting stratum corneum lipid structure. We further hypothesized that magainin's enhancement requires co-administration with a surfactant chemical enhancer to increase magainin penetration into the skin. In support of these hypotheses, exposure to a known surfactant chemical enhancer, N-lauroyl sarcosine (NLS), in 50% ethanol solution increased in vitro skin permeability to fluorescein 15 fold and the combination of magainin and NLS-ethanol synergistically increased skin permeability 47 fold. In contrast, skin permeability was unaffected by exposure to magainin without co-enhancement by NLS-ethanol. Furthermore, confocal microscopy showed that magainin in the presence of NLS-ethanol penetrated deeply and extensively into stratum corneum, whereas magainin alone penetrated poorly into the skin. Additional analysis by Fouriertransform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry showed that NLS-ethanol disrupted stratum corneum lipid structure and that the combination of magainin and NLS-ethanol disrupted stratum corneum lipids even further. Altogether, these data suggest that NLSethanol increased magainin penetration into stratum corneum, which further increased stratum corneum lipid disruption and skin permeability. We believe this is the first study to demonstrate the use of a pore-forming peptide to increase skin permeability. This study also introduces the novel concept of using a first chemical enhancer to increase penetration of a second chemical enhancer into the skin to synergistically increase skin permeability to a model drug.

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Section: Differential Scanning Calorimetrymentioning

confidence: 78%

Transdermal delivery enhanced by magainin pore-forming peptide

Kim

Ludovice

Prausnitz

2007

Journal of Controlled Release

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“…In the band between 3600-3300 cm -1 , we can observe the presence of water in the species' SC (Golden et al, 1986;Lin et al, 1992).…”

Section: Resultsmentioning

confidence: 80%

“…The phase transition of the lipid bilayer from lamellar gel to liquid state is attributed to the temperature of 75-85 °C. The transition that occurs at 105 °C represents the dehydration and denaturation of the protein fraction of the SC and the presence of a certain amount of water in the sample is required for its detection by the technique (Baby et al, 2006b;Golden et al, 1986, Ashton et al, 1992Leopold, Lipppold, 1995).…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Baby

Lacerda

Sarruf

et al. 2009

Braz. J. Pharm. Sci.

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Recently, there has been an interest in the use of shed snake skin as alternative model biomembrane for human stratum corneum. This research work presented as objective the qualitative characterization of alternative model biomembranes from Bothrops jararaca and Spilotis pullatus by FT-Raman, PAS-FTIR and DSC. The employed biophysical techniques permitted the characterization of the biomembranes from shed snake skin of B. jararaca and S. pullatus by the identification of vibrational frequencies and endothermic transitions that are similar to those of the human stratum corneum.Uniterms: Biomembrane/alternative models. Bothrops jararaca/skin/qualitative characterization. Spilotis pullatus/skin/qualitative characterization. Human stratum corneum/similars.Existe atualmente interesse no uso da muda de pele de cobra como modelos alternativos de biomembranas da pele humana. O presente trabalho apresentou como objetivo a caracterização qualitativa de modelos alternativos de biomembranas provenientes de mudas de pele de cobra da Bothrops jararaca e Spilotis pullatus por espectroscopia Raman (FT-Raman), espectroscopia fotoacústica no infravermelho (PAS-FTIR) e calorimetria exploratória diferencial (DSC). As técnicas biofísicas FT-Raman, PAS-FTIR e DSC permitiram caracterizar qualitativamente os modelos alternativos de biomembranas provenientes das mudas de pele de cobra da B. jararaca e S. pullatus e identificar freqüências vibracionais e transições endotérmicas similares ao estrato córneo humano.Unitermos: Biomembranas/modelos alternativos. Bothrops jararaca/pele/caracterização qualitativa. Spilotis pullatus/pele/caracterização qualitativa. Estrato córneo humano/similares.

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“…Increase in enthalpy and transition T m1 by Labrafil and Capryol may due to bilayer cohesion [20], which is in contrast to lipid extraction and fluidization. The shift in Tm to lower temperatures can be interpreted as disruption of the lipid bilayer and the irreversible denaturation of proteins in the SC layer of skin, while decrease in ∆H is related to fluidization of lipid in lipid bilayer and lipid -protein complex [14][15][16][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

“…They showed that Tm 1 corresponds to lipid transformation from a lamellar to disordered state, Tm 2 is due to the melting of lipid -protein( keratin) complex [2,16], or the disruption of polar head groups of lipids [14][15][16][17], Tm3 is known to occur during the irreversible denaturation of proteins in the SC [16].…”

Section: Differential Scanning Calorimetery (Dsc)mentioning

confidence: 99%