Lipid trafficking in plant cells

Moreau, Patrick; Bessoule, Jean‐Jacques; Mongrand, Sébastien; Testet, Eric; Vincent, Patrick W.; Cassagne, Claude

doi:10.1016/s0163-7827(98)00016-2

Cited by 152 publications

(129 citation statements)

References 150 publications

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“…[57]. Consistently, in plant, monensin, a secretory pathway inhibitor, was shown to cause accumulation of part of the plasma membrane lipids in the Golgi [1]. This pathway is actin dependent [45].…”

Section: Transfer To the Plasma Membranementioning

confidence: 71%

“…Finally, dissection of these mechanisms of transfer will be an important challenge for the future. Table 1: Lipid composition of plant cell membranes according to [1]. Endomembrane analyses are derived from [102], [103], and [104].…”

Section: Resultsmentioning

confidence: 99%

“…By using monensin, Moreau et al [1] tried to determine which lipid classes were transferred to plant plasma membrane via the vesicular pathway. When monensin was added, these authors observed in the plasma membrane the absence of PS, whereas the PI content was not affected, and PE and PC amounts were half reduced.…”

Section: Transfer To the Plasma Membranementioning

confidence: 99%

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Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

134

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Section: Transfer To the Plasma Membranementioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

134

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“…Plant sterols are synthesized in the ER membranes (Hartmann and Benveniste, 1987) and are transported from the ER to the PM via a membrane-mediated process, which likely involves the GA (Moreau et al, 1998b). This transport is blocked by low temperatures and compounds such as monensin or brefeldin A, as also reported for different phospholipid species, especially phosphatidyl-Ser (Moreau et al, 1998a(Moreau et al, , 1998bSturboisBalcerzak et al, 1999;Vincent et al, 1999).…”

mentioning

confidence: 83%

Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells

et al. 2006

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The existence of sphingolipid-and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton X-100)-resistant membranes (DRMs) from the PM of Arabidopsis (Arabidopsis thaliana) and leek (Allium porrum) seedlings as well as from Arabidopsis cell cultures. Here, we show that all DRM preparations are enriched in sterols, sterylglucosides, and glucosylceramides (GluCer) and depleted in glycerophospholipids. The GluCer of DRMs from leek seedlings contain hydroxypalmitic acid. We investigated the role of sterols in DRM formation along the secretory pathway in leek seedlings. We present evidence for the presence of DRMs in both the PM and the Golgi apparatus but not in the endoplasmic reticulum. In leek seedlings treated with fenpropimorph, a sterol biosynthesis inhibitor, the usual D 5 -sterols are replaced by 9b,19-cyclopropylsterols. In these plants, sterols and hydroxypalmitic acid-containing GluCer do not reach the PM, and most DRMs are recovered from the Golgi apparatus, indicating that D 5 -sterols and GluCer play a crucial role in lipid microdomain formation and delivery to the PM. In addition, DRM formation in Arabidopsis cells is shown to depend on the unsaturation degree of fatty acyl chains as evidenced by the dramatic decrease in the amount of DRMs prepared from the Arabidopsis mutants, fad2 and Fad31, affected in their fatty acid desaturases.Despite the ongoing debate on the size, lifespan, and dynamics of lipid microdomains (Munro, 2003;Pike, 2004;Nichols, 2005;Hancock, 2006), the existence of sterol-and sphingolipid-enriched membrane microdomains in the plasma membrane (PM) of eukaryotic cells is now well recognized (Simons and Ikonen, 1997;Brown and London, 2000;Simons and Toomre, 2000;Bagnat and Simons, 2002;Simons and Vaz, 2004;Hancock, 2006). Evidence for microdomains comes in part from examination of membrane constituents that are resistant to solubilization by nonionic detergents at low temperature, giving rise to the concept of lipid rafts (Simons and Ikonen, 1997;Brown and London, 1998;Rö per et al., 2000;Hancock, 2006). Such steroland sphingolipid-enriched microdomains can be isolated as detergent-resistant membranes (DRMs). In the following, the terms of DRMs and microdomains will be assigned to respectively indicate the isolated entities and the corresponding membrane entities. Lipid properties of microdomains are similar to liquid-ordered domains, which are characterized by tightly packed hydrocarbon tails but with a high degree of lateral mobility (Brown and London, 2000;Simons and Vaz, 2004). Cholesterol is thought to contribute to the tight packing of lipids in liquid-ordered domains by filling interstitial spaces between lipid molecules (Brown, 1998)

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“…Unlike other organelles, chloroplast membranes are rich in galactolipids and contain low levels of phospholipids (Moreau et al, 1998). Thus, some phospholipid species like phosphatidyl ethanolamine (PE) , which accounts for 20 to 40% of the total species present in extraplastidial membranes, are absent in chloroplast ones.…”

Section: Lipid Analysis Of Sunflower Leavesmentioning

confidence: 99%

Cloning, heterologous expression and biochemical characterization of plastidial sn-glycerol-3-phosphate acyltransferase from Helianthus annuus

et al. 2015

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The acyl-[acyl carrier protein]:sn-1-glycerol-3-phosphate acyltransferase (GPAT; E.C. 2.3. 1.15) catalyzes the first step of glycerolipid assembly within the stroma of the chloroplast. In the present study, the sunflower (Helianthus annuus, L.) stromal GPAT was cloned, sequenced and characterized. We identified a single ORF of 1344 base pairs that encoded a GPAT sharing strong sequence homology with the plastidial GPAT from Arabidopsis thaliana (ATS1, At1g32200). Gene expression studies showed that the highest transcript levels occurred in green tissues in which chloroplasts are abundant. The corresponding mature protein was heterologously overexpressed in Escherichia coli for purification and biochemical characterization. In vitro assays using radiolabelled acyl-ACPs and glycerol-3-phosphate as substrates revealed a strong preference for oleic versus palmitic acid, and weak activity towards stearic acid. The positional fatty acid composition of relevant chloroplast phospholipids from sunflower leaves did not reflect the in vitro GPAT specificity, suggesting a more complex scenario with mixed substrates at different concentrations, competition with other acyl-ACP consuming enzymatic reactions, etc. In summary, this study has confirmed the affinity of this enzyme affinity which would partly explain the resistance to cold temperatures observed in sunflower plants.

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Lipid trafficking in plant cells

Cited by 152 publications

References 150 publications

Glycerolipid transfer for the building of membranes in plant cells

Glycerolipid transfer for the building of membranes in plant cells

Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells

Cloning, heterologous expression and biochemical characterization of plastidial sn-glycerol-3-phosphate acyltransferase from Helianthus annuus

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