Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption

Svenning, Jon Brage; Dalheim, Lars; Vasskog, Terje; Matricon, L.; Vang, Birthe; Olsen, Ragnar L.

doi:10.1038/s41598-020-79269-z

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…The lipids in the diatom biomass have earlier been found to make up approximately 8-10% of the dry weight (20% of ash free dry weight) when harvested in the exponential growth phase [159,160], which is similar to the lipid content found in the present experiment where fume and compressed air solely were injected into the culture. In their review of the literature of lipid content of diatoms, Fields and Kociolek [161] reported that species cultivated in nutrient poor media contained 6.6-39.8% lipids (DW); however, their experimental data showed an average of 5.3-21.5% lipids (DW) when cultivated in nutrient replete media.…”

Section: Discussionsupporting

confidence: 86%

“…The slightly higher omega-3 content, caused by the elevated 18:4n-3 content in samples with factory flue gas, was the main difference. The EPA content seems to be stable at approximately 30% no matter the cultivation conditions [46,159,160,163,164]. Other diatoms usually contain 5-20% EPA; however, species such as Skeletonema costatum and Phaeodactylum tricornutum have been reported to contain 30% of this fatty acid [165].…”

Section: Discussionmentioning

confidence: 99%

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Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration

et al. 2022

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From 2015 to 2021, we optimized mass cultivation of diatoms in our own developed vertical column airlift photobioreactors using natural and artificial light (LEDs). The project took place at the ferrosilicon producer Finnfjord AS in North Norway as a joint venture with UiT—The Arctic University of Norway. Small (0.1–6–14 m3) reactors were used for initial experiments and to produce inoculum cultures while upscaling experiments took place in a 300 m3 reactor. We here argue that species cultivated in reactors should be large since biovolume specific self-shadowing of light can be lower for large vs. small cells. The highest production, 1.28 cm3 L−1 biovolume (0.09–0.31 g DW day−1), was obtained with continuous culture at ca. 19% light utilization efficiency and 34% CO2 uptake. We cultivated 4–6 months without microbial contamination or biofouling, and this we argue was due to a natural antifouling (anti-biofilm) agent in the algae. In terms of protein quality all essential amino acids were present, and the composition and digestibility of the fatty acids were as required for feed ingredients. Lipid content was ca. 20% of ash-free DW with high EPA levels, and omega-3 and amino acid content increased when factory fume was added. The content of heavy metals in algae cultivated with fume was well within the accepted safety limits. Organic pollutants (e.g., dioxins and PCBs) were below the limits required by the European Union food safety regulations, and bioprospecting revealed several promising findings.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration

et al. 2022

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“…Before the analysis of samples, it is of great significance to select a suitable method for better compound extraction. There are some research studies about extraction-assistance treatments, , which indicate that the treatments may influence the extraction results. The treated samples were processed with triglyceride (TG) assay kits, and the optical density of GEs at 510 nm (OD 510 nm) was measured as the index of GE yield.…”

Section: Resultsmentioning

confidence: 99%

SWATH-MS2&1: Development and Validation of a Pseudotargeted Lipidomics Method for the Analysis of Glycerol Esters in Milk

Sun

Shi

et al. 2022

J. Agric. Food Chem.

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Glycerol ester (GE) is a kind of important lipid in milk, which varies greatly depending on many factors. In this study, a novel pseudotargeted lipidomics strategy, named SWATH-MS2&1, was developed for the detection of GEs in milk and the Folch method was selected for the sample preparation. The developed method exhibited a competitive alternative to the acknowledged pseudotargeted strategy, including wider coverage (12 more GEs detected), higher repeatability (12 more GEs, whose coefficient of variation < 0.3), better linearity (5 more GEs, whose R 2 > 0.8), and similar sensitivity (only 2 GEs less than P-MRM after dilution). SWATH-MS2&1 was applied in the investigation of GEs from different milk samples. The orthogonal partial least-squares difference analysis of 219 GEs identified from SWATH-MS2&1 showed satisfying differentiation of different milk samples, and 76 GEs were screened out as potential markers. Our findings demonstrated that SWATH-MS2&1 could offer an accurate method to measure a wide spectrum of GEs in milk.

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“…Lipids and pigments were extracted from lyophilized material as described in [39]. Briefly, 100 mg of material was added in 20 volumes (2 mL) of DCM/MeOH (2:1 v/v) and 5% NaCl, respectively, and centrifuged at 2000× g for 5 min before transferring the organic phase to pre-weighed 4 mL glass vials and evaporating the solvents under nitrogen.…”

Section: Lipid and Pigment Extractionmentioning

confidence: 99%

Lipidome Plasticity Enables Unusual Photosynthetic Flexibility in Arctic vs. Temperate Diatoms

Svenning,

Vasskog,

Campbell

et al. 2024

Marine Drugs

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The diatom lipidome actively regulates photosynthesis and displays a high degree of plasticity in response to a light environment, either directly as structural modifications of thylakoid membranes and protein–pigment complexes, or indirectly via photoprotection mechanisms that dissipate excess light energy. This acclimation is crucial to maintaining primary production in marine systems, particularly in polar environments, due to the large temporal variations in both the intensity and wavelength distributions of downwelling solar irradiance. This study investigated the hypothesis that Arctic marine diatoms uniquely modify their lipidome, including their concentration and type of pigments, in response to wavelength-specific light quality in their environment. We postulate that Arctic-adapted diatoms can adapt to regulate their lipidome to maintain growth in response to the extreme variability in photosynthetically active radiation. This was tested by comparing the untargeted lipidomic profiles, pigmentation, specific growth rates and carbon assimilation of the Arctic diatom Porosira glacialis vs. the temperate species Coscinodiscus radiatus during exponential growth under red, blue and white light. Here, we found that the chromatic wavelength influenced lipidome remodeling and growth in each strain, with P. glacialis showing effective utilization of red light coupled with increased inclusion of primary light-harvesting pigments and polar lipid classes. These results indicate a unique photoadaptation strategy that enables Arctic diatoms like P. glacialis to capitalize on a wide chromatic growth range and demonstrates the importance of active lipid regulation in the Arctic light environment.

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Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption

Cited by 9 publications

References 44 publications

Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration

Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration

SWATH-MS2&1: Development and Validation of a Pseudotargeted Lipidomics Method for the Analysis of Glycerol Esters in Milk

Lipidome Plasticity Enables Unusual Photosynthetic Flexibility in Arctic vs. Temperate Diatoms

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