Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients

Malý, Martin; Hajšl, Martin; Bechyňská, Kamila; Kučerka, Ondřej; Šrámek, Martin; Suttnar, Jiřı́; Hlaváčková, Alžběta; Hajšlová, Jana; Kosek, Vít

doi:10.3390/metabo11070412

Cited by 13 publications

(10 citation statements)

References 26 publications

(32 reference statements)

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“…In our previous study [4], multiple significant changes in lipid patterns were observed five days after ACS. With regards to limited data in the literature, we were interested in long-term trends in respective patients' plasma lipidome.…”

Section: Discussionmentioning

confidence: 80%

“…Blood samples were collected from 17 patients 3-6 days after ACS (group B) and from the same patients three years after (group AC) who were diagnosed at the Central Military Hospital, University Military Hospital in Prague, Czech Republic (Table 2). These samples represent subgroups of 61 plasma samples that were analyzed from patients undergoing percutaneous coronary intervention (PCI) due to ACS described in our previous work [4]. Blood samples were collected via venipuncture.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting data showed that lysophosphoinositol (LPI) levels were significantly higher in ACS patients' plasma, indicating platelet activation. Higher levels of fatty acyl esters of hydroxy fatty acids (FAHFA) in controls might be due to higher anti-inflammatory activity [4].…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Effects on the Lipidome of Acute Coronary Syndrome Patients

et al. 2022

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Lipids modified by oxidative stress are key players in atherosclerosis progression. Superimposed thrombosis with subsequent closure of the coronary artery leads to the clinical manifestation of acute coronary syndrome (ACS). While several studies focusing on alterations in lipid metabolism in the acute phase have been conducted, no information is available on patients’ lipidome alterations over longer time periods. In the current follow-up study, we analyzed plasma samples obtained from 17 patients three years after their ACS event (group AC). Originally, these patients were sampled 3–5 days after an index event (group B). Lipidome stability over time was studied by untargeted lipidomics using high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC–HRMS). Multi-dimensional statistics used for data processing indicated that plasmalogen lipids were the most prominent lipids separating the above patient groups and that they increased in the follow-up AC group. A similar trend was observed for lysophosphatidylethanolamine (LPE) and phosphatidylethanolamine (PE). The opposite trend was observed for two fatty acyls of hydroxy fatty acid (FAHFAs) lipids and free stearic acid. In addition, a decrease in the “classic” oxitadive stress marker, malondialdehyde (MDA), occurred during the follow-up period. Our findings present unique information about long-term lipidome changes in patients after ACS.

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Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

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Long-Term Effects on the Lipidome of Acute Coronary Syndrome Patients

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“…OFRs lead to the formation of lipid peroxides which alter cell membranes by increasing incorporation of cholesterol and oxidized free fatty acids (FFAs) and low-density lipoproteins [ 78 ]. As for atherogenesis [ 90 ], chronic oxidative stress in the placenta leads to impaired circulation and diffuse maternal endothelial cell dysfunction. Abnormal endothelial function exemplified by increased circulating levels of fibronectin and von Willebrand factor, markers of endothelial cell injury, is found in women with PE.…”

Section: Consequences For the Pregnant Womanmentioning

confidence: 99%

Long-Term Consequences of Placental Vascular Pathology on the Maternal and Offspring Cardiovascular Systems

et al. 2021

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Over the last thirty years, evidence has been accumulating that Hypertensive Disorders of Pregnancy (HDP) and, specifically, Preeclampsia (PE) produce not only long-term effects on the pregnant woman, but have also lasting consequences for the fetus. At the core of these consequences is the phenomenon known as defective deep placentation, being present in virtually every major obstetrical syndrome. The profound placental vascular lesions characteristic of this pathology can induce long-term adverse consequences for the pregnant woman’s entire arterial system. In addition, placental growth restriction and function can, in turn, cause a decreased blood supply to the fetus, with long-lasting effects. Women with a history of HDP have an increased risk of Cardiovascular Diseases (CVD) compared with women with normal pregnancies. Specifically, these subjects are at a future higher risk of: Hypertension; Coronary artery disease; Heart failure; Peripheral vascular disease; Cerebrovascular accidents (Stroke); CVD-related mortality. Vascular pathology in pregnancy and CVD may share a common etiology and may have common risk factors, which are unmasked by the “stress” of pregnancy. It is also possible that the future occurrence of a CVD may be the consequence of endothelial dysfunction generated by pregnancy-induced hypertension that persists after delivery. Although biochemical and biophysical markers of PE abound, information on markers for a comparative evaluation in the various groups is still lacking. Long-term consequences for the fetus are an integral part of the theory of a fetal origin of a number of adult diseases, known as the Barker hypothesis. Indeed, intrauterine malnutrition and fetal growth restriction represent significant risk factors for the development of chronic hypertension, diabetes, stroke and death from coronary artery disease in adults. Other factors will also influence the development later in life of hypertension, coronary and myocardial disease; they include parental genetic disposition, epigenetic modifications, endothelial dysfunction, concurrent intrauterine exposures, and the lifestyle of the affected individual.

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“…Oxylipins influence numerous physiological and pathological processes [ 20 , 21 , 22 ]. Furthermore, the function of oxylipins in acute coronary syndrome (ACS) or other cardiovascular disorders has been reported [ 23 , 24 , 25 , 26 ]. Here, we demonstrated altered oxylipins in individuals with AD and healthy individuals.…”

Section: Introductionmentioning

confidence: 99%

Serum Oxylipin Profiles Identify Potential Biomarkers in Patients with Acute Aortic Dissection

et al. 2022

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Aortic dissection (AD) is a life-threatening cardiovascular disease with a dismal prognosis. Inflammation plays an important role in AD. Oxylipins are bioactive lipids involved in the modulation of inflammation and may be involved in the pathogenesis and progression of AD. This study aims to identify possible metabolites related to AD. A total of 10 type A Aortic dissection (TAAD) patients, 10 type B Aortic dissection (TBAD) patients and 10 healthy controls were included in this study. Over 100 oxylipin species were identified and quantified by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. Our investigation demonstrated substantial alterations in 91 oxylipins between AD and healthy individuals. Patients with TAAD had 89 entries accessible compared to healthy controls. According to orthogonal partial least squares discriminant analysis (OPLS-DA), fitness (R2X = 0.362 and R2Y = 0.807, p = 0.03) and predictability (Q2 = 0.517, p = 0.005) are the validation parameters between the two groups. Using multivariate logistic regression, 13-HOTrE and 16(17)-EpDPE were the risk factors in the aortic patients group compared to healthy people (OR = 2.467, 95%CI:1.256–7.245, p = 0.035; OR = 0.015, 95%CI:0.0002–0.3240, p = 0.016, respectively). In KEGG enrichment of differential metabolites, the arachidonic acid metabolism pathway has the most metabolites involved. We established a diagnostic model in distinguishing between AD and healthy people. The AUC was 0.905. Oxylipins were significantly altered in AD patients, suggesting oxylipin profile is expected to exploit a novel, non-invasive, objective diagnosis for AD.

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Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients

Cited by 13 publications

References 26 publications

Long-Term Effects on the Lipidome of Acute Coronary Syndrome Patients

Long-Term Effects on the Lipidome of Acute Coronary Syndrome Patients

Long-Term Consequences of Placental Vascular Pathology on the Maternal and Offspring Cardiovascular Systems

Serum Oxylipin Profiles Identify Potential Biomarkers in Patients with Acute Aortic Dissection

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