Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics

Ekroos, Kim

doi:10.1002/9783527655946.ch1

Cited by 16 publications

(20 citation statements)

References 40 publications

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“…Based on the high resolution performance and accurate mass values obtained using the Orbitrap, the sum compositions 25 of the glycerolipid, sphingolipid, cholesterol, cholesterol ester and PI lipid ions, including (i) assignment of the head group identities, (ii) the nature of their hydrophobic chain linkages (i.e., diacyl versus plasmanyl- or plasmenyl-ether) and (iii) the total number of carbons and double bonds that were present within their hydrophobic chains, could be automatically assigned by in silico comparison with the calculated masses of a database of hypothetical lipid compounds, using the LIMSA software (Table 1 and Supplemental Tables 1–15). For example, m/z 850.7857 in Figure 1A was assigned as the ammonium ion adduct of a TG (50:1) lipid (calculated m/z 850.7858), with the experimental and calculated m/z values differing by only 0.0001 Da (0.08 ppm).…”

Section: Resultsmentioning

confidence: 99%

“…Mass spectra were acquired in positive and negative ionization modes using the FT analyzer operating at 100,000 resolving power. Assignment of the molecular lipid compositions 25 of selected ions from the underivatized sample was achieved using Higher-Energy Collision Induced Dissociation (HCD-MS/MS) and/or ion trap CID-MS/MS and -MS n . Further details are available in the attached Supplemental Information.…”

Section: Methodsmentioning

confidence: 99%

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Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

et al. 2012

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A ‘shotgun’ lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution / accurate mass spectrometry and ‘targeted’ tandem mass spectrometry (MS/MS) analysis has been developed and applied toward the comprehensive identification, characterization and quantitative analysis of changes in relative abundances of >600 individual glycerophospholipid, glycerolipid, sphingolipid and sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, and its isogenic lymph node metastasized derivative, SW620. Selective chemical derivatization of glycerophosphoethanolamine and glycerophosphoserine lipids using a ‘fixed charge’ sulfonium ion containing, d6-S,S′-dimethylthiobutanoylhydroxysuccinimide ester (d6-DMBNHS) reagent was used to eliminate the possibility of isobaric mass overlap of these species with the precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assignment, while subsequent selective mild acid hydrolysis of plasmenyl (vinyl-ether) containing lipids using formic acid enabled these species to be readily differentiated from isobaric mass plasmanyl (alkyl-ether) containing lipids. Using this approach, statistically significant differences in the abundances of numerous lipid species previously identified as being associated with cancer progression, or that play known roles as mediators in a range of physiological and pathological processes, were observed between the SW480 and SW620 cells. Most notably, these included increased plasmanylcholine and triglyceride lipid levels, decreased plasmenylethanolamine lipids, decreased C-16 containing sphingomyelin and ceramide lipid levels, and a dramatic increase in the abundances of total cholesterol ester and triglyceride lipids in the SW620 cells compared to those in the SW480 cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

et al. 2012

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“…407 (17), 2015. Recently edited books and monographs on the areas of lipid analysis and lipidomics [6, 33, 34] should be the useful resources.…”

Section: Recommended Lipidomics Resourcesmentioning

confidence: 99%

Lipidomics for precision medicine and metabolism: A personal view

Han

2017

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…4(c) and 4(d)) and, thus, Eqns. (5) and (6) can be used for a rough estimation of parameters of calibration curves for polyunsaturated PC as well.…”

Section: Response Dependences On Fatty Acyl Length and Db Numbermentioning

confidence: 99%

“…[2] These functions of PC are associated with several diseases connected with deviations from the normal lipid expression. [3][4][5][6] Quantitative lipidomic analysis can be used for disease biomarker discoveries. Electrospray ionization mass spectrometry (ESI-MS) is commonly used in lipidomic studies.…”

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confidence: 99%

Effects of fatty acyl chain length, double‐bond number and matrix on phosphatidylcholine responses in matrix‐assisted laser desorption/ionization on an Orbitrap mass spectrometer

Chagovets

Lísa

Holčapek

2015

Rapid Comm Mass Spectrometry

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Differences in relative responses for PC in MALDI-MS measurements must be taken into account for accurate quantitation. Parameters of calibration curves can be used for the determination of PC concentrations using a single internal standard (IS). This method gives good results for the 9AA matrix, but the reproducibility of measurements for the DHB and DAN matrices is lower and the method can be used for a rough estimation only. These matrices are less convenient for the quantitation of PC.

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Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics

Cited by 16 publications

References 40 publications

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

Lipidomics for precision medicine and metabolism: A personal view

Effects of fatty acyl chain length, double‐bond number and matrix on phosphatidylcholine responses in matrix‐assisted laser desorption/ionization on an Orbitrap mass spectrometer

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