2015
DOI: 10.1002/rcm.7404
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Effects of fatty acyl chain length, double‐bond number and matrix on phosphatidylcholine responses in matrix‐assisted laser desorption/ionization on an Orbitrap mass spectrometer

Abstract: Differences in relative responses for PC in MALDI-MS measurements must be taken into account for accurate quantitation. Parameters of calibration curves can be used for the determination of PC concentrations using a single internal standard (IS). This method gives good results for the 9AA matrix, but the reproducibility of measurements for the DHB and DAN matrices is lower and the method can be used for a rough estimation only. These matrices are less convenient for the quantitation of PC.

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Cited by 3 publications
(3 citation statements)
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“…The latter was then used to calculate the percent abundance of each isomer among all detected L‐NAPEs (see the sixth column in Table 2). Considering that the ionization yield of intact lipids can be affected by chain length and degree of unsaturation and that the yield of the fragmentation pathway leading to FA carboxylates depends on the sn ‐position in the glycerol backbone, 50 it has to be emphasized that data reported in Table 2 are only a rough estimate of the relative content of L‐NAPEs. Nevertheless, helpful insights into the relative occurrence of L‐NAPE isomers in the investigated sample were obtained.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The latter was then used to calculate the percent abundance of each isomer among all detected L‐NAPEs (see the sixth column in Table 2). Considering that the ionization yield of intact lipids can be affected by chain length and degree of unsaturation and that the yield of the fragmentation pathway leading to FA carboxylates depends on the sn ‐position in the glycerol backbone, 50 it has to be emphasized that data reported in Table 2 are only a rough estimate of the relative content of L‐NAPEs. Nevertheless, helpful insights into the relative occurrence of L‐NAPE isomers in the investigated sample were obtained.…”
Section: Resultsmentioning
confidence: 99%
“…Considering that the ionization yield of intact lipids can be affected by chain length and degree of unsaturation and that the yield of the fragmentation pathway leading to FA carboxylates depends on the snposition in the glycerol backbone, 50 it has to be emphasized that data reported in…”
Section: Analysis Of L-napes In Tomato Waste Lipid Extractsmentioning
confidence: 99%
“…The second reason was that 9-AA resulted in low background in the negative mode (as compared with DHB), offering a more reproducible quantitative analysis among the sample spots in the negative mode (Sun et al, 2008;Wang and Giese, 2017). The advantages of 9-AA over DHB were also reported in the PC quantitation in the positive mode (Chagovets et al, 2015), in the generation of abundant H + adducts, and in the moderate production of Na + adducts, which enabled simplified peak assignment during the analysis of complex mixtures (Pyttel et al, 2012). Therefore, 9-AA has been identified as one of the most suitable and effective matrices for the analysis of urinary lipids.…”
Section: Optimization Of Maldi Matrix In Positive and Negative Ionization Modesmentioning
confidence: 99%