Lipids and Lysosomes

Hamer, Isabelle; Beersel, Guillaume Van; Arnould, Thierry; Jadot, Michel

doi:10.2174/138920012803762684

Cited by 29 publications

(24 citation statements)

References 354 publications

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“…EE and LE exhibit differences in their protein and lipid composition, which reflect their different functions (Falguières et al ., ). The inner leaflet of the limiting membranes from early/recycling endosomes, are rich in SM, Cho, glycospingolipids and glycerophospholipids whereas those from LE/Lys contain a higher proportion of PC, lysobiphosphatidic acid (LBPA) and ceramide (Cer), as well as less phosphatidylserine (PS) and SM than those from EE (Hammer et al ., ). There are detergent‐resistant membrane domains on the cytosolic face of all endosomal compartments (Scita and Di Fiore, ; Pálfy et al ., ), and these domains serve as platforms for docking and assembly of signalling complexes, as occurs in the cytosolic leaflet of the PM (Scita and Di Fiore, ; Pálfy et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Internalization ofClostridium perfringensα-toxin leads to ERK activation and is involved on its cytotoxic effect

et al. 2013

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Clostridium perfringens phospholipase C (CpPLC), also called α-toxin, plays a key role in the pathogenesis of gas gangrene. CpPLC may lead to cell lysis at concentrations that cause extensive degradation of plasma membrane phospholipids. However, at sublytic concentrations it induces cytotoxicity without inducing evident membrane damage. The results of this work demonstrate that CpPLC becomes internalized in cells by a dynamin-dependent mechanism and in a time progressive process: first, CpPLC colocalizes with caveolin both at the plasma membrane and in vesicles, and later it colocalizes with early and late endosomes and lysosomes. Lysosomal damage in the target cells is evident 9 h after CpPLC exposure. Our previous work demonstrated that CpPLCinduces ERK1/2 activation, which is involved in its cytotoxic effect. In this work we found that cholesterol sequestration, dynamin inhibition, as well as inhibition of actin polymerization, prevent CpPLC internalization and ERK1/2 activation, involving endocytosis in the signalling events required for CpPLC cytotoxic effect at sublytic concentrations. These results provide new insights about the mode of action of this bacterial phospholipase C, previously considered to act only locally on cell membrane.

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Section: Discussionmentioning

confidence: 97%

Internalization ofClostridium perfringensα-toxin leads to ERK activation and is involved on its cytotoxic effect

et al. 2013

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“…[74][75][76] during which they are exposed to varying conditions, such as pH changes from 7.4 (in the extracellular medium), to 5.5 (in late endosomes), and finally to 4.5 (in lysosomes). 77 This altered environment can have several consequences, for example it was shown that bound or adsorbed proteins can be rapidly degraded. 78 Ag NPs, some of the most widely studied engineered NPs, were shown to dissolve in endosomes and lysosomes.…”

Section: Colloidal Interactions In Cell Culture Mediummentioning

confidence: 99%

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

et al. 2015

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Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to complex biological media containing electrolytes, proteins, lipids, etc., nanoparticles (NPs) are subjected to a range of forces which determine their behavior in this environment. One aspect of NP behavior in biological systems that is often understated or overlooked is aggregation. NP aggregation will significantly alter in vitro behavior (dosimetry, NP uptake, cytotoxicity), as well as in vivo fate (pharmacokinetics, toxicity, biodistribution). Thus, understanding the factors driving NP colloidal stability and aggregation is paramount. Furthermore, studying biological interactions with NPs at the nanoscale level requires an interdisciplinary effort with a robust understanding of multiple characterization techniques. This review examines the factors that determine NP colloidal stability, the various efforts to stabilize NP in biological media, the methods to characterize NP colloidal stability in situ, and provides a discussion regarding NP interactions with cells.

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“…Since lysosomes are the final destination for autophagy and other endocytotic pathways , endo/lysosomal dysfunction not only affects macroautophagy but also impairs CMA, recycling of membrane lipids, sorting of lipids and lipoproteins into appropriate intracellular compartments, and promotes lipotoxic cell death (23,154,155).…”

Section: Regulation Of Lysosomal Function By Lipidsmentioning

confidence: 99%

Lipids, lysosomes, and autophagy

Jaishy

Abel

2016

Journal of Lipid Research

178

139

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Lipids are essential components of a cell providing energy substrates for cellular processes, signaling intermediates, and building blocks for biological membranes. Lipids are constantly recycled and redistributed within a cell. Lysosomes play an important role in this recycling process that involves the recruitment of lipids to lysosomes via autophagy or endocytosis for their degradation by lysosomal hydrolases. The catabolites produced are redistributed to various cellular compartments to support basic cellular function. Several studies demonstrated a bidirectional relationship between lipids and lysosomes that regulate autophagy. While lysosomal degradation pathways regulate cellular lipid metabolism, lipids also regulate lysosome function and autophagy. In this review, we focus on this bidirectional relationship in the context of dietary lipids and provide an overview of recent evidence of how lipid-overload lipotoxicity, as observed in obesity and metabolic syndrome, impairs lysosomal function and autophagy that may eventually lead to cellular dysfunction or cell death.

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Lipids and Lysosomes

Cited by 29 publications

References 354 publications

Internalization ofClostridium perfringensα-toxin leads to ERK activation and is involved on its cytotoxic effect

Internalization ofClostridium perfringensα-toxin leads to ERK activation and is involved on its cytotoxic effect

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

Lipids, lysosomes, and autophagy

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