Lipids from visceral depot fat of Asian seabass (Lates calcarifer): Compositions and storage stability as affected by extraction methods

Abstract

Lipids from Asian seabass visceral depot fat (SVDF) were extracted using different methods including heating in air, heating under vacuum, autoclave, and solvent extraction. Lipid extracted by heating under vacuum had the highest yield (67.33%) (p < 0.05). All extracted lipids had triglyceride (TG) as the major component, followed by phospholipid (PL). Oleic acid (C18:1 n‐9) constituted as the most abundant fatty acid in all lipids, followed by palmitic acid (C16:0) and linoleic acid (C18:2 n‐6), regardless of extraction methods. Docosahexaenoic acid (C22:6 n‐3) and eicosapentaenoic acid (C20:5 n‐3) accounted for 6.88–7.41 and 1.76–2.51%, respectively. During storage of 30 days at 30°C, lipids extracted by heating under vacuum or by solvent had the higher oxidative stability as indicated by the lower peroxide value (PV), thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), and ρ‐anisidine value (AnV). Lower increases in free fatty acid (FFA) content were also found in the samples obtained by those two methods. Extraction of lipid by heating under vacuum or solvent could lower the formation of volatile lipid oxidation products. Thus, extraction method had the profound impact on yield and storage stability of lipids from SVDF. Practical applications: Asian seabass visceral depot fat is considered as the byproduct during butchering process. This fatty tissue can serve as the potential raw material for lipid production. Several methods have been used for extraction of lipids, in which yield and chemical compositions can be varied. Therefore, the appropriate extraction method should be implemented to increase the efficacy of lipid extraction and to obtain lipid with high quality and oxidative stability. As a consequence, Asian seabass viscera can be better exploited and fish lipid rich in n‐3 fatty acids can be produced and used as nutraceutical or supplement. Lipids from Asian seabass visceral depot fat are extracted using different methods including heating in air, heating under vacuum, autoclave, and solvent extraction. Extraction by heating under vacuum rendered the lipid with the highest yield and the oxidation occurred at the low extent. During storage of 30 days at 30°C, lipids extracted by heating under vacuum also have the high oxidative stability. Therefore, Asian seabass viscera could be the potential raw material of lipid rich in n‐3 fatty acids, which can be extracted and used as nutraceutical or supplement.

Ammonium Sulfate and Repeated Freeze‐Thawing Recover Oil from Emulsion Separated from Salmon Skin Hydrolysate

“…FFA was generated by the hydrolysis of glycerol‐fatty acid esters. [ 25 ] Free fatty acid with lower steric hindrance is susceptible to lipid oxidation than esterified counterpart. [ 19 ] High moisture and heat can induce hydrolysis of lipid.…”

Cellulose Nanocrystals Reinforced Zein/Catechin/β-Cyclodextrin Inclusion Complex Nanoparticles Nanocomposite Film for Active Food Packaging

“…The increase in the TBARS value revealed the formation of secondary oxidation products, because TBARS value is an index of decomposition of hydroperoxides into the secondary oxidation products in later stages of lipid oxidation [ 52 ]. Hydroperoxides are decomposed to malonaldehyde, resulting in the off-flavor of oxidized lipids [ 53 ]. It is worth noting that the TBARS value of oil in Zein/NPs was higher than that in films with CNCs throughout 30 days of storage.…”

Squalene from Fish Livers Extracted by Ultrasound‐Assisted Direct In Situ Saponification: Purification and Molecular Characteristics

“…Conversely, the oil yield from sea bass liver was significantly higher (14.75 ± 0.83 g [100 g] −1 ) when compared to that of gray bamboo shark and skipjack tuna. Apart from liver, the majority of the lipids in Asian sea bass were stored in the form of depot fat (Sae‐leaw and Benjakul, ). Among the traditional extraction methods known, solvent method has been widely used for total lipid extraction (Hamm et al, ).…”