Lipogenic capacity of liver from perinatal swine (Sus domesticus)

Mersmann, Harry J.; Phinney, G.; Sanguinetti, Michael C.; Houk, J.M.

doi:10.1016/0305-0491(73)90089-8

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…In addition, lipogenic activity is low in neonatal piglets [19] and is not affected by nutritional state [5], Consequently, con centrations of malonyl-CoA should have had minimal influence on mitochondrial P-oxidation in piglets.…”

Section: Discussionmentioning

confidence: 99%

Response of Hepatic Mitochondrial and Peroxisomal β-Oxidation to Increasing Palmitate Concentrations in Piglets

Yu¹,

Drackley²,

Odle³

et al. 1997

Neonatology

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Responses of total, mitochondrial, and peroxisomal β-oxidation to increasing [1-¹⁴C]-palmitate concentrations (0.02–1.0 mM) were measured in liver homogenates from neonatal pigs. Incubations were conducted in the absence (total β-oxidation) or presence (peroxisomal β-oxidation) of antimycin A and rotenone; mitochondrial β-oxidation was calculated as total minus peroxisomal oxidation. Total and mitochondrial β-oxidations were maximized at a palmitate concentration of 0.05 mM, whereas peroxisomal β-oxidation was maximized at 0.50 mM palmitate. Across concentrations, peroxisomal β-oxidation contributed 40–47% of total β-oxidation. An increased rate of CO₂ production and a greater ratio of CO₂ production to total mitochondrial β-oxidation as palmitate concentration increased suggested that the limited capacity for mitochondrial β-oxidation was attributable primarily to limited ketogenic capacity. Comparative observations in liver from adult rats showed that peroxisomal β-oxidation was maximized at 0.1 mM palmitate, but total and mitochondrial β-oxidation rates were not maximized even at 1 mM palmitate. At 1 mM palmitate, peroxisomal β-oxidation was 20% of total β-oxidation in adult rats and 37% in adult pigs. Therefore, the contribution of peroxisomal β-oxidation to total β-oxidation is highly dependent on substrate concentration and appears to be greater in adult pigs than in adult rats. The greater proportional contribution of peroxisomal β-oxidation in piglet liver might act as a compensatory mechanism for piglets to oxidize milk fatty acids.

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Section: Discussionmentioning

confidence: 99%

Response of Hepatic Mitochondrial and Peroxisomal β-Oxidation to Increasing Palmitate Concentrations in Piglets

Yu¹,

Drackley²,

Odle³

et al. 1997

Neonatology

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Hepatic ketogenesis in newborn pigs is limited by low mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase activity

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et al. 1994

Biochemical Journal

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In newborn-pig hepatocytes, the rate of oleate oxidation is extremely low, despite a very low malonyl-CoA concentration. By contrast, the sensitivity of carnitine palmitoyltransferase (CPT) I to malonyl-CoA inhibition is high, as suggested by the very low concentration of malonyl-CoA required for 50% inhibition of CPT I (IC50). The rates of oleate oxidation and ketogenesis are respectively 70 and 80% lower in mitochondria isolated from newborn-pig liver than from starved-adult-rat liver mitochondria. Using polarographic measurements, we showed that the oxidation of oleoyl-CoA and palmitoyl-L-carnitine is very low when the acetyl-CoA produced is channelled into the hydroxymethylglutaryl-CoA (HMG-CoA) pathway by addition of malonate. In contrast, the oxidation of the same substrates is high when the acetyl-CoA produced is directed towards the citric acid cycle by addition of malate. We demonstrate that the limitation of ketogenesis in newborn-pig liver is due to a very low amount and activity of mitochondrial HMG-CoA synthase as compared with rat liver mitochondria, and suggest that this could promote the accumulation of acetyl-CoA and/or beta-oxidation products that in turn would decrease the overall rate of fatty acid oxidation in newborn- and adult-pig livers.

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Metabolic Patterns in the Neonatal Swine

Mersmann

1974

Journal of Animal Science

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Lipogenic capacity of liver from perinatal swine (Sus domesticus)

Cited by 4 publications

References 9 publications

Response of Hepatic Mitochondrial and Peroxisomal β-Oxidation to Increasing Palmitate Concentrations in Piglets

Response of Hepatic Mitochondrial and Peroxisomal β-Oxidation to Increasing Palmitate Concentrations in Piglets

Hepatic ketogenesis in newborn pigs is limited by low mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase activity

Metabolic Patterns in the Neonatal Swine

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