Metabolic Patterns in the Neonatal Swine

Mersmann, H. J.

doi:10.2527/jas1974.3851022x

Cited by 66 publications

(53 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, changes in gene expression of FABP3 and FABP4 were not observed until day 14 in skeletal muscle when reduced expression was seen in all offspring that were not within the normal ranges of birth weight. The emergence of differences in FABP3 and 4 in skeletal muscle at an older age compared to adipose tissue may reflect the fact that carbohydrate, rather than lipid, metabolism in skeletal muscle (and liver) is crucial during the first week of life in the pig due to the lack of lipid reserves at birth (Mersmann 1974, Mitchell et al 2001. In the first 14 days of life, adipose tissue in the pig becomes unilocular with a substantial accumulation of triglycerides (Mersmann 1974), changes requiring cooperation with FABPs as well as PPARG.…”

Section: Fabp3 and Fabp4mentioning

confidence: 99%

“…The emergence of differences in FABP3 and 4 in skeletal muscle at an older age compared to adipose tissue may reflect the fact that carbohydrate, rather than lipid, metabolism in skeletal muscle (and liver) is crucial during the first week of life in the pig due to the lack of lipid reserves at birth (Mersmann 1974, Mitchell et al 2001. In the first 14 days of life, adipose tissue in the pig becomes unilocular with a substantial accumulation of triglycerides (Mersmann 1974), changes requiring cooperation with FABPs as well as PPARG. It is very unlikely that the presence of FABP4 in skeletal muscle could be due to the accumulation of intramuscular adipocytes at this early stage of development.…”

Section: Fabp3 and Fabp4mentioning

confidence: 99%

See 1 more Smart Citation

Influence of birth weight on gene regulators of lipid metabolism and utilization in subcutaneous adipose tissue and skeletal muscle of neonatal pigs

Williams¹,

Marten²,

Wilson³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Epidemiological studies suggest that low-birth weight infants show poor neonatal growth and increased susceptibility to metabolic syndrome, in particular, obesity and diabetes. Adipose tissue development is regulated by many genes, including members of the peroxisome proliferator-activated receptor (PPAR) and the fatty acid-binding protein (FABP) families. The aim of this study was to determine the influence of birth weight on key adipose and skeletal muscle tissue regulating genes. Piglets from 11 litters were ranked according to birth weight and 3 from each litter assigned to small, normal, or large-birth weight groups. Tissue samples were collected on day 7 or 14. Plasma metabolite concentrations and the expression of PPARG2, PPARA, FABP3, and FABP4 genes were determined in subcutaneous adipose tissue and skeletal muscle. Adipocyte number and area were determined histologically. Expression of FABP3 and 4 was significantly reduced in small and large, compared with normal, piglets in adipose tissue on day 7 and in skeletal muscle on day 14. On day 7, PPARA and PPARG2 were significantly reduced in adipose tissue from small and large piglets. Adipose tissue from small piglets contained more adipocytes than normal or large piglets. Birth weight had no effect on adipose tissue and skeletal muscle lipid content. Low-birth weight is associated with tissue-specific and time-dependent effects on lipid-regulating genes as well as morphological changes in adipose tissue. It remains to be seen whether these developmental changes alter an individual's susceptibility to metabolic syndrome.

show abstract

Section: Fabp3 and Fabp4mentioning

confidence: 99%

Section: Fabp3 and Fabp4mentioning

confidence: 99%

Influence of birth weight on gene regulators of lipid metabolism and utilization in subcutaneous adipose tissue and skeletal muscle of neonatal pigs

Williams¹,

Marten²,

Wilson³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…As previously mentioned, serum AGP concentrations increase during late gestation in the pig and are highest at birth (Lampreave and Piñeiro, 1992), then rapidly decline postnatally Heegaard et al, 2013). The interaction of AGP with insulin to reduce insulin-mediated lipogenesis may be especially important in the fetal pig during late gestation when lipid accumulation within adipose tissue is restricted due to the relative unavailability of exogenous lipids and the consequent reliance on glucose for de novo lipogenesis in the fetus (Mersmann, 1974). Lipid accretion in the fetal pig has been demonstrated to respond to exogenous insulin (Garssen et al, 1983); however, under physiological conditions there is minimal lipid accretion (Mersmann et al, 1975).…”

Section: Agp and Lipogenesis In Neonatal Pigsmentioning

confidence: 97%

α 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue

Ramsay

Blomberg

Caperna

2016

Animal

View full text Add to dashboard Cite

Serum α1-acid glycoprotein (AGP) is elevated during late gestation and at birth in the pig and rapidly declines postnatally. In contrast, the pig is born with minimal lipid stores in the adipose tissue, but rapidly accumulates lipid during the first week. The present study examined if AGP can affect adipose tissue metabolism in the neonatal pig. Isolated cell cultures or tissue explants were prepared from dorsal subcutaneous adipose tissue of preweaning piglets. Porcine AGP was used at concentrations of 0, 100, 1000 and 5000 ng/ml medium in 24 h incubations. AGP reduced the messenger RNA (mRNA) abundance of the lipogenic enzymes, malic enzyme (ME), fatty acid synthase and acetyl coA carboxylase by at least 40% ( P < 0.001). The activity of ME and citrate lyase were also reduced by AGP ( P < 0.05). Glucose oxidation was reduced by treatment with 5000 ng AGP/ml medium ( P < 0.05). The 14 C-glucose incorporation into fatty acids was reduced by~25% by AGP treatment for 24 h with 1000 ng AGP/ml medium ( P < 0.05). The decrease in glucose metabolism by AGP appears to function through an inhibition in insulin-mediated glucose oxidation and incorporation into fatty acids. This was supported by the analysis of the mRNA abundance for sterol regulatory element-binding protein (SREBP), carbohydrate regulatory element-binding protein (ChREBP) and insulin receptor substrate 1 (IRS1), which all demonstrated reductions of at least 23% in response to AGP treatment ( P < 0.05). These data demonstrate an overall suppression of lipogenesis due to AGP inhibition of lipogenic gene expression in vitro, which the metabolic data and SREBP, ChREBP and IRS1 gene expression analysis suggest is through an inhibition in insulin-mediated events. Second, these data suggest that AGP may contribute to limiting lipogenesis within adipose tissue during the perinatal period, as AGP levels are highest for any serum protein at birth.

show abstract

“…Thus, although these authors quote the values as a % of those in adults [adult values : --120 mg/100 ml and -180 mg/100 ml of serum for LDL and HDL, respectively ; Chapman (1980)], calculation showed LDL and HDL levels to be about 80 and 50 mg/100 ml, respectively, in early fetal life (-70 days). Subsequently and at the end of the fetal period, HDL predominated slightly (-70 Mersmann (1974) who reported that the capacity to produce endogenous fatty acids for energy generation is limited in newborn piglets.…”

mentioning

confidence: 99%