Lipolytic enzymes of the human pancreas. II. Purification and properties of cholesterol ester hydrolase

Meyer, Jürgen G.

doi:10.1016/0005-2760(89)90069-6

Cited by 15 publications

(7 citation statements)

References 20 publications

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“…Cholesterol esterase is also called carboxylic ester hydrolase or sterol ester hydrolase. It has been purified from porcine (108,109), rat (110), and human pancreas (111,112). Human cholesterol esterase has a molecular weight of about 100 kDa (111), whereas rat cholesterol esterase has a molecular weight of 70 kDa (110).…”

Section: Digestion Of Cholesteryl Estermentioning

confidence: 99%

Intestinal lipid absorption and transport

Phan¹

2001

Front Biosci

156

117

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Introduction 3. Dietary lipids 4. Structured lipids 5. Digestion of dietary lipids and structured lipids 5.1. Gastric lumen 5.2. Intestinal lumen 5.3. Digestion of phospholipid 5.4. Digestion of cholesteryl ester 6. Uptake of dietary lipids by enterocytes 6.1. Importance of micellar solubilization 6.2. Importance of unilamellar vesicles 6.3. Uptake of fat digestion products by enterocytes 7. Resynthesis of triglycerides and formation of chylomicrons 7.1. Monoglycerides and fatty acid 7.2. Phospholipids 7.3. Cholesterol 8. Assembly of intestinal lipoproteins 9. Clinical disorders of intestinal lipid transport 10. Acknowledgements 11. References

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Section: Digestion Of Cholesteryl Estermentioning

confidence: 99%

Intestinal lipid absorption and transport

Phan¹

2001

Front Biosci

156

117

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“…The presence of short forms of BSDL in human pancreatic juice has been reported by Meyer [26], and Duang and Borgström [27]. Furthermore in 2000, Pasqualini et al showed that MiaPaCa-2 cells expressed an immunoreactive form of BSDL of approximately 70 kDa, which corresponds to the 1.8 kb cDNA obtained by RT-PCR using a pair of primers covering the full-length mRNA encoding the human BSDL [28].…”

Section: Introductionmentioning

confidence: 88%

Expression of truncated bile salt-dependent lipase variant in pancreatic pre-neoplastic lesions

et al. 2016

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Pancreatic adenocarcinoma (PDAC) is a dismal disease. The lack of specific symptoms still leads to a delay in diagnosis followed by death within months for most patients. Exon 11 of the bile salt-dependent lipase (BSDL) gene encoding variable number of tandem repeated (VNTR) sequences has been involved in pancreatic pathologies. We hypothesized that BSDL VNTR sequences may be mutated in PDAC. The amplification of BSDL VNTR from RNA extracted from pancreatic SOJ-6 cells allowed us to identify a BSDL amplicon in which a cytosine residue is inserted in a VNTR sequence. This insertion gives rise to a premature stop codon, resulting in a truncated protein and to a modification of the C-terminal amino-acid sequence; that is PRAAHG instead of PAVIRF. We produced antibodies directed against these sequences and examined pancreatic tissues from patients with PDAC and PanIN. Albeit all tissues were positive to anti-PAVIRF antibodies, 72.2% of patient tissues gave positive reaction with anti-PRAAHG antibodies, particularly in dysplastic areas of the tumor. Neoplastic cells with ductal differentiation were not reactive to anti-PRAAHG antibodies. Some 70% of PanIN tissues were also reactive to anti-PRAAHG antibodies, suggesting that the C insertion occurs early during pancreatic carcinogenesis. Data suggest that anti-PRAAHG antibodies were uniquely reactive with a short isoform of BSDL specifically expressed in pre-neoplastic lesions of the pancreas. The detection of truncated BSDL reactive to antibodies against the PRAAHG C-terminal sequence in pancreatic juice or in pancreatic biopsies may be a new tool in the early diagnosis of PDAC.

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“…The enzyme involved in the hydrolyzation process is cholesterol esterase, also known as carboxylic ester hydrolase or sterol ester hydrolase. It has been purified from porcine [92,93], rat [94], and human [95,96] pancreas. Human cholesterol esterase has a molecular weight of about 100 kDa [95], whereas rat cholesterol esterase has a molecular weight of 70 kDa [94].…”

Section: Digestion Of Cholesteryl Estermentioning

confidence: 99%

“…The self-association of cholesterol esterase protects the enzyme from proteolytic inactivation, so retaining its activity. Cholesterol esterase isolated from the pancreas exists mainly as dimers and tetramers [96].…”

Section: Digestion Of Cholesteryl Estermentioning

confidence: 99%