Lipopeptides from Bacillus amyloliquefaciens strain 32a as promising biocontrol compounds against the plant pathogen Agrobacterium tumefaciens

Abdallah, Dorra Ben; Tounsi, Slim; Gharsallah, Houda; Hammami, Adnane; Frikha‐Gargouri, Olfa

doi:10.1007/s11356-018-3570-1

Cited by 35 publications

(30 citation statements)

References 53 publications

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“…As an excellent biocontrol agent, Bacillus amyloliquefaciens exhibits a broad spectrum of antifungal activities with the ability to produce different types of cyclic lipopeptides. Cyclic lipopeptides produced by B. amyloliquefaciens are catalyzed by non-ribosomal peptide synthase 3 , which inhibit the growth of various plant pathogenic bacteria and fungi 4 – 7 . Cyclic lipopeptides include lipopeptide surfactin, iturins, and fengycins 8 .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens

Yang

Zhang

Xiang

et al. 2020

Sci Rep

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Biological control mechanisms of plant diseases have been intensively studied. However, how plant pathogens respond to and resist or alleviate biocontrol agents remains largely unknown. in this study, a comparative transcriptome analysis was performed to elucidate how the pathogen of sclerotinia stem rot, Sclerotinia sclerotiorum, responds and resists to the biocontrol agent, Bacillus amyloliquefaciens. Results revealed that a total of 2,373 genes were differentially expressed in S. sclerotiorum samples treated with B. amyloliquefaciens fermentation broth (tS) when compared to control samples (CS). Among these genes, 2,017 were upregulated and 356 were downregulated. further analyses indicated that various genes related to fungal cell wall and cell membrane synthesis, antioxidants, and the autophagy pathway were significantly upregulated, including glucan synthesis, ergosterol biosynthesis pathway, fatty acid synthase, heme-binding peroxidase related to oxidative stress, glutathione S-transferase, ABc transporter, and autophagy-related genes. these results suggest that S. sclerotiorum recruits numerous genes to respond to or resist the biocontrol of B. amyloliquefaciens. thus, this study serves as a valuable resource regarding the mechanisms of fungal pathogen resistance to biocontrol agents.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens

Yang

Zhang

Xiang

et al. 2020

Sci Rep

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“…Two Pseudomonas fluorescens lipopeptides putisovin I and II are reported to suppress the biofilm formation and breakdown of existing biofilm [170]. Abdallah et al [171] reported the suppression of Agrobacterium tumefaciens biofilm by a mixture of lipopeptides produced by Bacillus amyloliquifaciens. The mixture maybe able to inhibit tumor formation upon pathogen adhesion on a tomato stem.…”

Section: Lipopeptides As Biosurfactants Distressing Membrane Integritmentioning

confidence: 99%

Lesson from Ecotoxicity: Revisiting the Microbial Lipopeptides for the Management of Emerging Diseases for Crop Protection

Malviya

Sahu

Singh

et al. 2020

IJERPH

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Microorganisms area treasure in terms of theproduction of various bioactive compounds which are being explored in different arenas of applied sciences. In agriculture, microbes and their bioactive compounds are being utilized in growth promotion and health promotion withnutrient fortification and its acquisition. Exhaustive explorations are unraveling the vast diversity of microbialcompounds with their potential usage in solving multiferous problems incrop production. Lipopeptides are one of such microbial compounds which havestrong antimicrobial properties against different plant pathogens. These compounds are reported to be produced by bacteria, cyanobacteria, fungi, and few other microorganisms; however, genus Bacillus alone produces a majority of diverse lipopeptides. Lipopeptides are low molecular weight compounds which havemultiple industrial roles apart from being usedas biosurfactants and antimicrobials. In plant protection, lipopeptides have wide prospects owing totheirpore-forming ability in pathogens, siderophore activity, biofilm inhibition, and dislodging activity, preventing colonization bypathogens, antiviral activity, etc. Microbes with lipopeptides that haveall these actions are good biocontrol agents. Exploring these antimicrobial compounds could widen the vistasof biological pest control for existing and emerging plant pathogens. The broader diversity and strong antimicrobial behavior of lipopeptides could be a boon for dealing withcomplex pathosystems and controlling diseases of greater economic importance. Understanding which and how these compounds modulate the synthesis and production of defense-related biomolecules in the plants is a key question—the answer of whichneeds in-depth investigation. The present reviewprovides a comprehensive picture of important lipopeptides produced by plant microbiome, their isolation, characterization, mechanisms of disease control, behavior against phytopathogens to understand different aspects of antagonism, and potential prospects for future explorations as antimicrobial agents. Understanding and exploring the antimicrobial lipopeptides from bacteria and fungi could also open upan entire new arena of biopesticides for effective control of devastating plant diseases.

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“…The methods for prevention and control of banana fusarium include chemical control (Bauer et al 2016), breeding of resistant varieties (Ambrico and Trupo 2017), agricultural control measures (Ben Abdallah et al 2018) and biological control (Alijani et al 2019). The first three measures have disadvantages, such as environmental pollution, high costs and unstable control effects (Jeong et al 2016;Hennessy et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…New highefficiency biocontrol strains need to be constantly added. The biocontrol mechanisms of biocontrol bacteria include competition (Zouari et al 2016), antagonism (Nandi et al 2017), and induction of plant resistance (Ben Abdallah et al 2018). Among them, antagonism is the main mechanism of biocontrol.…”

Section: Introductionmentioning

confidence: 99%

Antifungal mechanism of Streptomyces ma. FS‐4 on fusarium wilt of banana

Duan

Chen

Pang

et al. 2020

J. Appl. Microbiol.

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Aim: Research on prevention and cure of banana wilt is important to ensure the healthy development of the banana industry. In this study, antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana was investigated. Methods and Results: The physiological strain of banana fusarium pathogen Fusarium oxysporum f. sp. cubense Race 4 (FOC.4) was used as the target fungus, and the antifungal mechanism of the crude extract of Streptomyces ma. FS-4 was investigated. Eighteen different compounds identified by gas chromatography-mass spectrometry were composed of aldehydes, methyl, hydrocarbons, amides, esters and acids. FS-4 significantly inhibited the spore germination of the target fungi, with an EC 50 of 22Á78 μg ml −1. After treatment with 100 μg ml −1 FS-4 crude extract, the N-acetylglucosamine content in the mycelium increased 1Á95-fold. However, the extract had no significant effect on β-1,3-glucanase. At the FS-4 crude extract dose of 100 μg ml −1 , the total sugar and protein contents decreased by 28Á6 and 29Á1% respectively, and the fat content was 41Á3%. FS-4 significantly inhibited the activity of the mitochondrial complex III of Foc4, which was reduced by 52Á45%. Moreover FS-4 reduced the activity of succinate dehydrogenase, a key enzyme in the Krebs cycle, by 60Á2%. However, FS-4 had no significant effect on malate dehydrogenase. The membrane potential on the mitochondrial inner membrane was significantly reduced at the test concentration of 100 μg ml −1. ROS gradually accumulated in the Foc4 hypha, and the burst was 3Á97 times higher than the control. Conclusions: This study demonstrated that the antifungal mechanism of Streptomyces ma. FS-4 against Foc4 includes the destruction of the plasma membrane and mitochondrial dysfunction and finally induction of cell apoptosis. Significance and Impact of the Study: These results may indicate the prevention and control of banana wilt, which is of great significance to the healthy development of banana industry system.

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Lipopeptides from Bacillus amyloliquefaciens strain 32a as promising biocontrol compounds against the plant pathogen Agrobacterium tumefaciens

Cited by 35 publications

References 53 publications

Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens

Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens

Lesson from Ecotoxicity: Revisiting the Microbial Lipopeptides for the Management of Emerging Diseases for Crop Protection

Antifungal mechanism of Streptomyces ma. FS‐4 on fusarium wilt of banana

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