Lipoperoxides as an index of free radical activity in bone marrow transplant recipients

Abstract

It has been previously demonstrated that the conditioning therapy given to bone marrow transplant (BMT) recipients creates a high oxidant stress, resulting in a measured reduction in antioxidants, such as glutathione peroxidase (GSH-Px), vitamin E, and cell peroxide fragilities. As part of a current intervention trial of antioxidant therapy in BMT recipients, plasma thiobarbituric acid reactive substances (TBARS) were measured to assess peroxidation and free radical activity. Measurements were performed before and after conditioning therapy, and then at weekly intervals for a period of 6 wk after transplantation in 20 patients (10 controls and 10 antioxidant therapy [AOT] recipients). The TBARS results were compared with concurrent measurements of more specific elements of the antioxidant pathways, such as red blood cell glutathione peroxidase (RBC-GSH-Px), plasma vitamin C, and serum vitamin E. In all cases, TBARS concentration was significantly increased after conditioning compared with baseline levels (p < 0.001), an increase that correlated inversely with RBC-GSH-Px (r = -0.81; p < 0.01). The TBARS concentration fell gradually after conditioning in all patients. The fall in the AOT group was more rapid than in the control group, and it paralleled the gradual return toward normal levels of the other antioxidants. The change in TBARS concentration occurred faster than changes in other indices, suggesting that TBARS might be a better index of overall free radical activity. Although the patient numbers are small, there is some evidence to suggest that MDA may act as a prognostic marker.(ABSTRACT TRUNCATED AT 250 WORDS)

Evaluation of oxidative and antioxidative parameters in pediatric hematopoietic SCT patients

“…However, several studies showed that antioxidants do not interfere with and can actually enhance the killing capabilities of cancer therapeutic modalities and decrease their side effects. [3][4][5][6][7] Conditioning therapy preceding HSCT usually consists of high-dose chemotherapy. A limited number of studies has shown that high-dose chemotherapy decreased antioxidant enzyme and vitamin levels in patients undergoing HSCT.…”

“…They have severe acute and delayed toxic effects on several tissues, such as mucous membranes, gastrointestinal tract, liver, lung, bladder, central nervous system, and, rarely, other tissues possibly related to peroxidation processes and exhaustion of antioxidants. [3][4][5][6][7] It is well known that chemotherapy and radiation therapy are associated with the formation of reactive oxygen species and depletion of antioxidants, such as glutathione (GSH), antioxidant enzymes, and antioxidant vitamins such as a-tocopherol, b-carotene and vitamin C. 4,[8][9][10][11] A limited number of studies demonstrated that the conditioning therapy given to HSCT patients creates a high oxidative stress, resulting in a measured reduction in antioxidants. In summary, these studies revealed a disturbance of the pro-oxidative/antioxidative balance in the plasma of patients undergoing HSCT.…”

“…Therefore, it has been suggested that high-dose chemotherapy increased the oxidative stress and decreased the antioxidant defence system. [3][4][5][6][7][8][9][10][11] The objective of this study was to evaluate the oxidative and antioxidative status in pediatric allo-HSCT patients.…”

Increases in 4-hydroxynonenal and hexanal in bone marrow of rats subjected to total body X-ray irradiation: association with antioxidant vitamins

“…It has been reported that BMT was not successful in patients with high plasma TBA-RS concentrations following irradiation. 12 The findings suggest that the prevention of lipid peroxidation at sites other than the BM is important for successful transplantation. Przybyszewski et al 30 reported that the TBA-RS concentration in serum and heart increased about 4 days after fractionated gammairradiation, and the increase was diminished by vitamin E treatment.…”

“…In their study, they evaluated lipid peroxidation by the thiobarbituric acid (TBA) method which has been criticized for its lack of specificity. 9,10 Although the concentrations of antioxidants in plasma have been reported to decrease following TBI in humans, 5,11,12 changes in the concentrations of antioxidants and the association of these changes with lipid peroxidation have not been clarified, particularly in the BM. Accordingly, it is of interest to determine how lipid peroxidation occurs in the BM following irradiation, and whether lipid peroxidation is influenced by antioxidant treatment.…”