Lipoplexes Targeting the CD44 Hyaluronic Acid Receptor for Efficient Transfection of Breast Cancer Cells

Surace, Claudio; Berlier, Gloria; Dufaÿ-Wojcicki, Amélie; Marsaud,; Bouclier, Céline; Clay, Denis; Cattel, Luigi; Jm, Renoir; Fattal, Elias

doi:10.1021/mp800215d

Cited by 147 publications

(114 citation statements)

References 46 publications

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“…Previous studies by Surace et al [17] have shown that 10% HA-DOPE (w/w) was optimal for transfection of MDA-MB231 breast cancer cells expressing CD44. We have therefore used this amount of conjugate and formulated lipoplexes at different lipids:DNA ratios.…”

Section: Discussionmentioning

confidence: 96%

“…We have synthesized a HA-DOPE conjugate which was included in lipoplexes demonstrating an improved transfection of breast cancer cells when these cells express the CD44 receptor [17]. These first results have demonstrated that using HA was indeed relevant for increasing the expression of a reporter gene in a cancer cell line.…”

Section: Introductionmentioning

confidence: 97%

“…All this has led to consider the high molecular weight HA as a "bioinert" component [14]. Most interestingly, HA was proposed as an alternative to PEG, to target liposomes encapsulating small molecules [15] and [16] or lipoplexes [17]. HA presents two advantages.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Wojcicki

Hillaireau

Nascimento

et al. 2012

Journal of Controlled Release

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The mechanism by which hyaluronic acid (HA)-bearing lipoplexes target the A549 lung cancer cell line was evaluated. For this purpose, cationic liposomes targeting the CD44 receptor were designed thanks to the incorporation in their composition of a conjugate between high molecular weight HA and the lipid DOPE (HA-DOPE). Liposomes containing HA-DOPE were complexed at different lipids:DNA ratios with a reporter plasmid encoding the green fluorescent protein (GFP). Diameter, zeta potential, lipoplex stability and DNA protection from nucleases have been determined.Lipids:DNA ratios of 2, 4 and 6 provided a diameter around 250 nm with a zeta potential of − 30 mV.The strength of lipids:DNA interaction and the fraction of DNA protected from enzymatic degradation increased with the lipids:DNA ratio. 2D-immunoelectrophoresis demonstrated the low capacity to activate the C3 fraction of the complement system of any of these three ratios, with and without HA-DOPE. Transfection efficiency in the presence of 0, 10 and 15% of HA-DOPE or unconjugated HA, was determined on the CD44-expressing A549 cells by flow cytometry.Lipoplexes at a lipids:DNA ratio of 2 containing 10% (w/w) of HA-DOPE were the most efficient for transfection. The maximal level of GFP expression was obtained after 6 h of incubation demonstrating a slow transfection kinetics of lipoplexes. Finally, lipoplex cellular uptake, measured indirectly by the level of transfection using flow cytometry and validated by fluorescence microscopy, was shown to be mediated by the CD44 receptor and caveolae. These results demonstrate the strong specificity of DNA targeting through the CD44 receptor using HA of high molecular weight as a ligand.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

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Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Wojcicki

Hillaireau

Nascimento

et al. 2012

Journal of Controlled Release

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“…Optimization of the molecular weight of HA, and the use of a pH sensitive cationic lipid are some promising candidates. Other groups demonstrated that liposomes containing a HA-DOPE lipid conjugate had targeting ability for CD44 expressed cells in vitro, 20,21) however, these studies were only evaluated in vitro.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Evaluation of Stearylated Hyaluronic Acid for the Active Delivery of Liposomes to Liver Endothelial Cells

Toriyabe

Hayashi

Hyodo

et al. 2011

Biological & Pharmaceutical Bulletin

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Hyaluronic acid (HA) is a naturally-occurring ligand that can be useful for targeting liver endothelial cells. We describe herein the development of a new HA-lipid conjugate for the efficient delivery of liposomes to liver endothelial cells. When free HA coated cationic liposomes were injected into mice, their accumulation in the liver was significantly decreased depending on the content of free HA, while accumulation in the lung was not significantly changed. When cationic liposomes modified with HA-stearylamine (HA-SA conjugate) were injected in mice, liver accumulation was increased depending on the amount of HA-SA conjugate and accumulation in the lung was drastically reduced, compared to non-modified liposomes. Confocal imaging analyses showed that HA-SA modified liposomes were accumulated to a greater extent along with blood vessels than non-modified liposomes, suggesitng that HA-SA modified liposomes are distributed in endothelial cells in the liver. Collectively, these findings indicate that an HA-SA conjugate is a useful material that can be used to modify liposomes and for delivering bioactive liposomal cargoes to liver endothelial cells.

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“…In addition to its moisturizing properties, hyaluronic acid (HA) and its endogenous receptor cluster of differentiation CD 44 , a transmembrane glycoprotein, have been linked to tumor growth, invasion, and metastasis. 21 Drug delivery systems have been developed to combine the specificity of the HA/CD 44 interaction with their respective carrier and targeting functions. 22,23 MSN has good potential as a carrier for drug delivery due to its mesoporous structure, high specific surface area, biocompatibility, and high DL capacity.…”

Section: Introductionmentioning

confidence: 99%

Natural material-decorated mesoporous silica nanoparticle container for multifunctional membrane-controlled targeted drug delivery

Hu¹,

Ke²,

Chen³

et al. 2017

IJN

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To avoid the side effects caused by nonspecific targeting, premature release, weak selectivity, and poor therapeutic efficacy of current nanoparticle-based systems used for drug delivery, we fabricated natural material-decorated nanoparticles as a multifunctional, membrane-controlled targeted drug delivery system. The nanocomposite material coated with a membrane was biocompatible and integrated both specific tumor targeting and responsiveness to stimulation, which improved transmission efficacy and controlled drug release. Mesoporous silica nanoparticles (MSNs), which are known for their biocompatibility and high drug-loading capacity, were selected as a model drug container and carrier. The membrane was established by the polyelectrolyte composite method from chitosan (CS) which was sensitive to the acidic tumor microenvironment, folic acid-modified CS which recognizes the folate receptor expressed on the tumor cell surface, and a CD 44 receptor-targeted polysaccharide hyaluronic acid. We characterized the structure of the nanocomposite as well as the drug release behavior under the control of the pH-sensitive membrane switch and evaluated the antitumor efficacy of the system in vitro. Our results provide a basis for the design and fabrication of novel membrane-controlled nanoparticles with improved tumor-targeting therapy.

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Lipoplexes Targeting the CD44 Hyaluronic Acid Receptor for Efficient Transfection of Breast Cancer Cells

Cited by 147 publications

References 46 publications

Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Synthesis and Evaluation of Stearylated Hyaluronic Acid for the Active Delivery of Liposomes to Liver Endothelial Cells

Natural material-decorated mesoporous silica nanoparticle container for multifunctional membrane-controlled targeted drug delivery

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