Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli

Kušećek, Barica; Wloch, H; Mercer, Andrew A.; Väisänen, Vuokko; Pluschke, Gerd; Korhonen, Timo; Achtman, Mark

doi:10.1128/iai.43.1.368-379.1984

Cited by 92 publications

(61 citation statements)

References 31 publications

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“…SDS-PAGE, in combination with Western blotting, has been revealed as a useful technique for LPS analysis [11,20,21]. Most of the samples recognized the O-repetitive chains (upper side of the NC membranes) and also the region corresponding to core and lipid A (down side).…”

Section: Discussionmentioning

confidence: 99%

“…Based on the fact only a limited number of serotypes, such as O4, O6, O7 and O16, is found in invasive infections [7][8][9][10][11], and that enteropathogenic E. coli presenting serotype O111:His one of the most frequent aetiological agents of infantile acute diarrhoea in developing countries [12], we proposed to investigate the presence of IgG and IgG subclass antibodies against purified E. coli LPS O6, O16 and O111 in paired maternal and cord sera.…”

Section: Introductionmentioning

confidence: 99%

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Placental Transfer of IgG and IgG Subclass Antibodies Anti‐Purified Escherichia coli LPS O16, O6 and O111

et al. 1998

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We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity-blotting for spectrotypic analysis, and the Western-blotting technique for recognition of the various bands in lipopolysaccharide (LPS). Levels of anti-LPS IgG antibodies in cord sera were equivalent to their respective maternal sera, showing a significant correlation (P < 0.0001). IgG1 antibody levels were higher in cord sera than in maternal sera (P < 0.005 for anti-O111, P < 0.05 for anti-O16 and P < 0.02 for anti-O6). Cord IgG2 antibody levels were not different from the maternal levels (P > 0.1). The levels of IgG3 and IgG4 were undetectable. The avidity of anti-O6 and anti-O111 IgG in 10 cord sera showed an extremely significant correlation with maternal antibody avidity (P < 0.0001). Identical patterns of recognition were found in the paired samples analysed by Western blotting. Most of the serum samples recognized the O-repetitive chains and also the region corresponding to core and lipid A. Although the antibody spectrotypes varied among individuals, paired cord and maternal serum samples showed identical patterns. Our findings suggest the occurrence of placental transfer of IgG antibodies against LPS O6, O16 and O111, mainly involving the IgG1 or IgG2 subclasses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Placental Transfer of IgG and IgG Subclass Antibodies Anti‐Purified Escherichia coli LPS O16, O6 and O111

et al. 1998

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“…Most of the Kl isolates belong to a limited spectrum of O-lipopolysaccharide (LPS) antigen types. Data have been presented elsewhere showing that pyelonephritis and meningitis in newborns can be differentially associated with Kl bacteria of different 0 serotypes (14,24). Here we concentrate on their virulence with regard to newborn meningitis.…”

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confidence: 99%

“…Thus, many babies carry Kl bacteria, but only a small percentage of the babies colonized with potentially virulent strains develop bacteremia and meningitis. Because of the low frequency of disease after gastrointestinal colonization and because disease isolates do not seem to differ from fecal isolates of the same serotype (1,14,18,22,24,27), it appears that host rather than parasite factors largely determine the outcome. Transplacentally transferred immunoglobulin G (IgG) specific for bacterial antigens may play a crucial role.…”

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confidence: 99%

Antibodies to O-antigen of lipopolysaccharide are protective against neonatal infection with Escherichia coli K1

Pluschke¹,

Achtman²

1985

Infect Immun

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Monoclonal IgM specific for the 018 antigen conferred passive protection to 1-week-old rats against bacteremia and killing after oral challenge with 018:K1 Escherichia coli. Specific protection of the pups was also achieved by immunizing the pregnant rats with purified 018 lipopolysaccharide. We suppose that most human newborns that are colonized by potentially invasive Kl E. coli are protected by the transplacental transfer of anti-lipopolysaccharide immunoglobulin G, and we suggest that treatment with such antibodies might in the future be considered a therapeutic option. Rat serum from 1-week-old animals had only about one-third of the complement hemolytic activity of adult rat serum. This low level of hemolytic activity correlated with a relatively poor bactericidal activity in antibody-dependent and antibody-independent bactericidal in vitro assays. Monoclonal anti-018 immunoglobulin M, although protective in vivo and bactericidal when added to adult rat serum, only poorly inhibited the multiplication of 018:K1 cells in serum from 1-week-old rats. This suggests that other elements of host defense besides complement participate in antibody-mediated in vivo protection.on July 15, 2020 by guest http://iai.asm.org/ Downloaded from

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“…P fimbriae from a number of E. coli strains have been purified and characterized immunologically (20), and a number of P fimbriae gene clusters have been cloned into E. coli K-12 and characterized in detail (2, 16,22,31). The E. coli strains carrying P fimbriae have been further analyzed for a number of properties, including serotypes, outer membrane proteins, and possession of other putative virulence factors and adhesin types (14,30).…”

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Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney

Korhonen¹,

Virkola²,

Holthöfer³

1986

Infect Immun

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Binding sites in the human kidney for purified P fimbriae of pyelonephritogenic Eschenichia coli were determined. The purified KS71A (F71) fimbriae bound only to epithelial elements of the kidney, i.e., to the apical aspect of proximal and distal tubular cells, as well as to the apical and cytoplasmic sites of collecting ducts. In addition, binding was seen at the vascular endothelium throughout the kidney and at the parietal epithelium of the glomeruli. The binding was specifically inhibited by the receptor analog of E. coli P fimbriae, globotriose. The binding sites identified suggested a possible pathogenetic mechanism for the invasion of P-fimbriated bacteria into the renal parenchyma, as well as for their subsequent spread into the circulatory system. G (Dakopatts a/s, Glostrup, Denmark) diluted 1:40 in PBS. 328 on July 4, 2020 by guest http://iai.asm.org/ Downloaded from LITERATURE CITED 1. Brinton, C. C. 1965. The structure, function, synthesis and genetic control of bacterial pili and a molecular mechanism for DNA and RNA transport in Gram-negative bacteria.

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Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli

Cited by 92 publications

References 31 publications

Placental Transfer of IgG and IgG Subclass Antibodies Anti‐Purified Escherichia coli LPS O16, O6 and O111

Placental Transfer of IgG and IgG Subclass Antibodies Anti‐Purified Escherichia coli LPS O16, O6 and O111

Antibodies to O-antigen of lipopolysaccharide are protective against neonatal infection with Escherichia coli K1

Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney

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