Lipopolysaccharide From Bacteroides Melaninogenicus Isolated From the Supernatant Fluid After Ultracentrifugation of the Water Phase Following Phenol‐water Extraction

Hofstad, Tor; Kristoffersen, T.

doi:10.1111/j.1699-0463.1971.tb00027.x

Acta Pathologica Microbiologica Scandinavica Section B Microbio

1971

DOI: 10.1111/j.1699-0463.1971.tb00027.x

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Lipopolysaccharide From Bacteroides Melaninogenicus Isolated From the Supernatant Fluid After Ultracentrifugation of the Water Phase Following Phenol‐water Extraction

Tor Hofstad

T. Kristoffersen

Abstract: Lipopolysaccharide (LPS) has been isolated from the supernatant fluid aftcr ultracentrifugation of the water phase following phenol-water extraction of Bncteroides meloninogenicus, strain B10. The purification procedure included digestion of the supernatant fluid with ribonuclease and deoxyribonuclease, gel filtration on Bio-Gel A-1.5 m and ion exchange chromatography on DEAE-cellulose. The prepared LPS did not contain heptose or 2-keto-3-deoxyoctulosonic acid. The sugar components identified were galactose, g… Show more

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Cited by 8 publications

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Endotoxins of gram-negative anaerobic bacteria

1980

Infection

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Background: Periodontal disease is an infectious disease that attacks gingiva and other dental supporting tissue which caused by microbial bacteria in subgingival plaque. The majority bacteria are an anaerobic gram-negative. Propolis is one of natural products as an antimicrobial mouthwash. One type of bee that able to produce propolis in large quantities is Trigona sp. which is common in South Sulawesi. Objective: The aim of this research is to know the effectiveness of propolis Trigona sp. as a mouthwash against some colonies of anaerobic gram-negative bacteria. Method: The type of this research is used pretest-posttest with control group design. A sample size of 28 people was selected with the research criteria who visited the Periodontology Department of Dental Hospital of Hasanuddin University. The treatment group rinse using extract propolis 5% and 10% and aquades as a control. Negative anarobic bacterial carried out from sulcus gingival fluid, then cultivated in medium MacConkey using spread method. Statistical analyzes used were T paired, and Anova test (p<0,05). Result: Paired T-test results showed that there was a difference of the number of anaerobic gram-negative bacteria colonies between before and after 14 days of treatment in the propolis group of 5% and 10% concentration (p = 0,000), when in the control group (aquades) there was no difference (p = 0.057). Anova test results showed that there was a decrease in the number of anaerobic gram-negative bacteria colonies between 5%, 10% propolis and control group (p = 0,000). Conclusion: Extract propolis Trigona sp. 5% and 10% which is contained in mouthwash is effective in lowering the number of anaerobic gram-negative bacteria colonies. The use of mouthwash with extractives of propolis may be recommended as an alternative therapy for periodontal disease.

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Endotoxins of gram-negative anaerobic bacteria

1980

Infection

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Antibodies Reacting with Lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis, and Fusobacterium nucleatum in Serum from Normal Human Subjects

Hofstad

1974

Journal of Infectious Diseases

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Cell Envelope Components of Strains Belonging to the Genus Thermus

Pask-Hughes¹,

Williams

1978

Journal of General Microbiology

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Envelopes of Thermus strains had a multilayered Gram-negative appearance. Peptidoglycan contained significant amounts of glycine, and ornithine was the dibasic amino acid. Lipopolysaccharide (LPS) did not contain heptose and 2-keto-3-deoxyoctonate was not positively identified. The polysaccharide from LPS contained glucose, glucosamine, galactose, galactosamine and, in two strains, mannose. The fatty acids identified in lipopolysaccharide were C14, C16 and CIS, both saturated and unsaturated.

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Lipopolysaccharide From Bacteroides Melaninogenicus Isolated From the Supernatant Fluid After Ultracentrifugation of the Water Phase Following Phenol‐water Extraction

Cited by 8 publications

References 20 publications

Endotoxins of gram-negative anaerobic bacteria

Endotoxins of gram-negative anaerobic bacteria

Antibodies Reacting with Lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis, and Fusobacterium nucleatum in Serum from Normal Human Subjects

Cell Envelope Components of Strains Belonging to the Genus Thermus

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